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Human TBP / GTF2D ELISA Kit (Sandwich ELISA) - LS-F36668

Catalog Size Price
LS-F36668-1 1 plate Unavailable

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Human TBP / GTF2D ELISA Kit (Sandwich ELISA) - LS-F36668
Quantitative Sandwich ELISA
96-Well Strip Plate
Human
0.313-20 ng/ml
Colorimetric - 450nm (TMB)
Serum, Tissue Homogenates, Plasma

100% Guaranteed
LS-F36668
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
TBP / GTF2D
Human
LS-F36668 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human TBP / GTF2D in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of TBP / GTF2D as low as 0.188 nanograms per millilter.
Plasma, Serum, Tissue Homogenates
96-Well Strip Plate
Colorimetric - 450nm (TMB)
Quantitative
0.313 - 20 ng/ml
0.188
Intra-Assay: CV<8% Inter-Assay: CV<10%
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
  • Adhesive Plate Sealers
  • Coated 96-well Strip Plate
  • Detection Antibody Diluent
  • Biotinylated Detection Antibody (100x)
  • HRP-Streptavidin Conjugate (100x)
  • HRP-Streptavidin Conjugate Diluent
  • Sample Diluent
  • TMB Substrate
  • Stop Solution
  • Wash Buffer (25x)
  • Standard (Lyophilized)
    This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- TAF antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- TAF antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TAF amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TAF can be calculated.
Short term: 4°C; Long term: see manual.
For research use only.

About TBP / GTF2D

P20226 NM_003194 NP_003185.1

TBP ElisaKit, GTF2D1 ElisaKit, HDL4 ElisaKit, TATA box binding protein ElisaKit, TATA-binding factor ElisaKit, TATA-box factor ElisaKit, TFIID ElisaKit, TATA sequence-binding protein ElisaKit, SCA17 ElisaKit, TATA-box-binding protein ElisaKit, GTF2D ElisaKit, TF2D ElisaKit

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals.

 

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Requested From: 
Date Requested: 5/23/2018

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