Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
The antibody pair provided in this kit recognizes human EGFR.
LS-F334 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Semi-Quantitative detection of Human EGFR in samples of Cell Lysates and Tissue Homogenates. It is based upon a Sandwich Phosphorylation assay principle.
All LSBio Custom kits have been predesigned and all necessary components, including antibodies and standards have been identified. Upon receiving a custom kit order, the kit will be assembled and quality control tested before being shipped out. Kit assembly and testing typically takes 4 to 6 weeks. In most cases the final kit is based on the Sandwich assay principle, with a few being Competitive EIA based. Specifications such as Range, Sensitivity, and Precision are defined upon completion. In the event that the custom kit cannot be successfully developed with 6 weeks of the order date, the customer will be notified and the order canceled at no cost.
Cell Lysates, Tissue Homogenates
Colorimetric - 450nm (TMB)
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
EGFR Microplate - Item A
Wash Buffer Concentrate (20x) - Item B
Anti-phospho-EGFR (Tyr 1045) - Item C
HRP-conjugated Anti-rabbit IgG - Item D-1
Assay Diluent (Item E)
Stop Solution - Item I
Cell Lysate Buffer - Item J
Positive Control A431S002-1 - Item K
Adhesive Plate Sealers
Upon receipt, the kit should be stored at –20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), HRP-conjugated Anti-rabbit IgG (Item D-1), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Anti-phospho-EGFR (Tyr 1045) (Item C) should be stored at –20 °C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at –20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues.