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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Human NF-L / NEFL ELISA Kit (Competitive EIA) - LS-F38902
Catalog
Size
Price
LS-F38902-1
1 plate
$630
Available Discounts
10% off 5+ units

Popular NF-L / NEFL Elisa Kits

Reactivity: Human
Range: 15.6-1000 pg/ml
Reactivity: Mouse
Range: 15.6-1000 pg/ml
Reactivity: Human
Range: 31.2-2000 pg/ml
Reactivity: Rat
Range: 78-5000 pg/ml
Reactivity: Rat
Range: 15.63-1000 pg/ml

Product Description

LS-F38902 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human NF-L / NEFL in samples of Cell Culture Supernatants, Cell Lysates, Cerebrospinal Fluid, Plasma, Serum and Tissue Homogenates. It is based upon a Competitive EIA assay principle and can be used to detect levels of NF-L / NEFL as low as 46.4 picograms per milliliter.

Specifications

Type
Competitive EIA (enzyme immunoassay) kit
Target
NF-L / NEFL
Synonyms
NEFL, 68 kDa neurofilament protein, CMT1F, CMT2E, NF68, NF-L, NFL, Neurofilament subunit NF-L
Reactivity
Human
Manual
Intended Sample Types
Cell Culture Supernatants, Cell Lysates, Cerebrospinal Fluid, Plasma, Serum, Tissue Homogenates
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
123.5 - 10000 pg/ml
Sensitivity
46.4
Assay Time
2.5 h
Precision
Intra-Assay: CV<10% Inter-Assay: CV<12%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • Coated 96-well Strip Plate
  • Adhesive Plate Sealers
  • Sample Diluent
  • Assay Diluent A
  • Assay Diluent B
  • Wash Buffer (30x)
  • TMB Substrate
  • Stop Solution
  • Standard (Lyophilized)
  • Detection Reagent A (Green)
  • Detection Reagent B (Red)
Restrictions
For research use only.
About NF-L / NEFL
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. P07196 NM_006158 NP_006149.2


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Requested From: United States
Date Requested: 10/20/2018

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