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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C157408-400 400 µl $325 
ZHX2 / RAF Antibody - Fluorescent confocal image of A549 cell stained with ZHX2 Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ZHX2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). ZHX2 immunoreactivity is localized to Nucleus significantly and Cytoplasm weakly.
ZHX2 / RAF Antibody - ZHX2 Antibody western blot of ZR-75-1 cell line lysates (35 ug/lane). The ZHX2 antibody detected the ZHX2 protein (arrow).
ZHX2 / RAF Antibody - Fluorescent confocal image of A549 cell stained with ZHX2 Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ZHX2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). ZHX2 immunoreactivity is localized to Nucleus significantly and Cytoplasm weakly.
ZHX2 / RAF Antibody - ZHX2 Antibody western blot of ZR-75-1 cell line lysates (35 ug/lane). The ZHX2 antibody detected the ZHX2 protein (arrow).
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Polyclonal ZHX2 / RAF Antibody (aa105‑131, IF, WB) LS‑C157408

Polyclonal ZHX2 / RAF Antibody (aa105‑131, IF, WB) LS‑C157408

Antibody:
Rabbit Polyclonal to Human ZHX2 / RAF
Application:
IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$325 
LS-C157408-400
400 µl
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human ZHX2 / RAF
Application:
IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
RAF antibody LS-C157408 is an unconjugated rabbit polyclonal antibody to human RAF (ZHX2) (aa105-131). Validated for IF and WB.
Target
Human ZHX2 / RAF
Synonyms
ZHX2 | AFR1 | Alpha-fetoprotein regulator 1 | AFP regulator 1 | KIAA0854 | Regulator of AFP | Transcription factor ZHX2 | Zinc fingers and homeoboxes 2 | Zinc-fingers and homeoboxes 2 | RAF
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
Blocking Peptide
LS-E6132 - Lyophilized - 500 µg - $360.00
Immunizing peptide used to generate LS-C157408. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa105-131
Specificity
This ZHX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 105-131 amino acids from the N-terminal region of human ZHX2.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ZHX2 / RAF
Q9Y6X8 NM_014943 NP_055758.1

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Requested From: United States
Date Requested: 3/28/2020