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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ZHX2 / RAF

zinc fingers and homeoboxes 2

The members of the zinc fingers and homeoboxes gene family are nuclear homodimeric transcriptional repressors that interact with the A subunit of nuclear factor-Y (NF-YA) and contain two C2H2-type zinc fingers and five homeobox DNA-binding domains. This gene encodes member 2 of this gene family. In addition to forming homodimers, this protein heterodimerizes with member 1 of the zinc fingers and homeoboxes family.

Gene Name: zinc fingers and homeoboxes 2
Synonyms: ZHX2, AFR1, Alpha-fetoprotein regulator 1, AFP regulator 1, KIAA0854, Regulator of AFP, Transcription factor ZHX2, Zinc fingers and homeoboxes 2, Zinc-fingers and homeoboxes 2, RAF
Target Sequences: NM_014943 NP_055758.1 Q9Y6X8

Filtered by: None
Target
ZHX2 / RAF (36)
Reactivity
Human (36)
Mouse (15)
Rat (10)
Bovine (4)
Chimpanzee (3)
Dog (8)
Gibbon (3)
Horse (4)
Monkey (5)
Orangutan (3)
Rabbit (8)
Application
IHC (7)
IHC-P (4)
WB (32)
ELISA (11)
ICC (1)
IF (2)
IP (3)
Peptide-ELISA (1)
RNAi (1)
Host
rabbit (29)
mouse (7)
Isotype
IgG (22)
IgG1 (4)
IgG2b (2)
IgG2b,k (1)
Clonality
monoclonal mc (7)
polyclonal pc (29)
Clone
5E2 (1)
OTI2E11 (2)
OTI2E2 (2)
OTI9A5 (2)
Format
AP Conjugated (1)
APC Conjugated (1)
Azide-free (1)
Biotin Conjugated (4)
Carrier-free (3)
FITC Conjugated (4)
HRP Conjugated (4)
PE Conjugated (1)
Unconjugated (21)
Epitope
aa105-131 (8)
aa595-837 (6)
C-Terminus (5)
N-Terminus (4)
aa703-752 (4)
720-800 aa, C-terminal (1)
aa533-826 (1)
aa737-787 (1)
aa751-800 (1)
aa787-837 (1)
Publications
No (36)
Showing 1-25 of 36 results

IHC-plus™ ZHX2 / RAF Antibody (aa751-800) LS-B11377
Rabbit Polyclonal (IgG) to Human ZHX2 / RAF
Mouse, Human
IHC, IHC-P, Peptide-ELISA
Unconjugated
100µl 1mg/ml  $505.00
Anti-ZHX2 / RAF antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.

ZHX2 / RAF Antibody (aa533-826) LS-C186253
Rabbit Polyclonal (IgG) to Human ZHX2 / RAF
Human
ICC, IHC, IHC-P, WB
Unconjugated
100µl 0.86mg/ml  $355.00
IHC of paraffin-embedded SAS Xenograft using ZHX2 antibody at 1:100 dilution.
Immunofluorescence of paraformaldehyde-fixed HeLa using ZHX2 antibody at 1:200 dilution.

ZHX2 / RAF Antibody (clone 5E2) LS-C133692
Mouse Monoclonal [clone 5E2] (IgG2b,k) to Human ZHX2 / RAF
Human
ELISA, IF, IHC, IHC-P, RNAi, WB
Unconjugated
100µg  $365.00
Immunoperoxidase of monoclonal antibody to ZHX2 on formalin-fixed paraffin-embedded human smooth muscle. [antibody concentration 3 ug/ml]
Immunofluorescence of monoclonal antibody to ZHX2 on HeLa cell. [antibody concentration 10 ug/ml]

IHC-plus™ ZHX2 / RAF Antibody (C-Terminus) LS-B13956
Rabbit Polyclonal to Human ZHX2 / RAF
Mouse, Rat, Human, Monkey
IHC, IHC-P, WB
Unconjugated
50µl 1mg/ml  $425.00
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot analysis of ZHX2 expression in HepG2 (A); K562 (B) whole cell lysates.

ZHX2 / RAF Antibody (aa595-837, clone OTI2E11) LS-C795554
Mouse Monoclonal [clone OTI2E11] (IgG2b) to Human ZHX2 / RAF
Mouse, Human
IHC, WB
Unconjugated
100µl 1mg/ml  $345.00
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-ZHX2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.5) at 120°C for 3 min. (1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ZHX2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.5) at 120°C for 3 min. (1:150)

ZHX2 / RAF Antibody (aa595-837, clone OTI2E11, Carrier-free) LS-C792530
Mouse Monoclonal [clone OTI2E11] (IgG2b) to Human ZHX2 / RAF
Mouse, Human
IHC, WB
Unconjugated, Carrier-free
100µg  $395.00
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-ZHX2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.5) at 120°C for 3 min. (1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ZHX2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.5) at 120°C for 3 min. (1:150)

ZHX2 / RAF Antibody (720-800 aa, C-terminal) LS-C386713
Rabbit Polyclonal (IgG) to Human ZHX2 / RAF
Mouse, Human
ELISA, IHC
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $335.00

ZHX2 / RAF Antibody LS-C660722
Rabbit Polyclonal (IgG) to Human ZHX2 / RAF
Human
ELISA, IP, WB
Unconjugated
100µg  $435.00

ZHX2 / RAF Antibody (aa105-131) LS-C157408
Rabbit Polyclonal to Human ZHX2 / RAF
Human
IF, WB
Unconjugated
400µl  $325.00
Fluorescent confocal image of A549 cell stained with ZHX2 Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ZHX2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). ZHX2 immunoreactivity is localized to Nucleus significantly and Cytoplasm weakly.
ZHX2 Antibody western blot of ZR-75-1 cell line lysates (35 ug/lane). The ZHX2 antibody detected the ZHX2 protein (arrow).

ZHX2 / RAF Antibody (aa105-131) LS-C330310
Rabbit Polyclonal (IgG) to Human ZHX2 / RAF
Human
ELISA, WB
Unconjugated
200µl  $590.00

ZHX2 / RAF Antibody (aa105-131, Biotin) LS-C224730
Rabbit Polyclonal (IgG) to Human ZHX2 / RAF
Human
ELISA, WB
Biotin Conjugated
200µl  $740.00

ZHX2 / RAF Antibody (aa105-131, FITC) LS-C224729
Rabbit Polyclonal (IgG) to Human ZHX2 / RAF
Human
ELISA, WB
FITC Conjugated
200µl  $740.00

ZHX2 / RAF Antibody (aa105-131, AP) LS-C224726
Rabbit Polyclonal (IgG) to Human ZHX2 / RAF
Human
ELISA, WB
AP Conjugated
200µl  $740.00

ZHX2 / RAF Antibody (aa105-131, APC) LS-C224727
Rabbit Polyclonal (IgG) to Human ZHX2 / RAF
Human
ELISA, WB
APC Conjugated
200µl  $740.00

ZHX2 / RAF Antibody (aa105-131, Azide-free, HRP) LS-C224728
Rabbit Polyclonal (IgG) to Human ZHX2 / RAF
Human
ELISA, WB
HRP Conjugated, Azide-free
200µl  $740.00

ZHX2 / RAF Antibody (aa105-131, PE) LS-C224732
Rabbit Polyclonal (IgG) to Human ZHX2 / RAF
Human
ELISA, WB
PE Conjugated
200µl  $740.00

ZHX2 / RAF Antibody LS-C807448
Rabbit Polyclonal (IgG) to Human ZHX2 / RAF
Mouse, Human
ELISA, WB
Unconjugated
100µl 1mg/ml  $325.00; 200µl 1mg/ml  $375.00
Western blot Image

ZHX2 / RAF Antibody (C-Terminus) LS-C31433
Rabbit Polyclonal (IgG) to Mouse ZHX2 / RAF
Rabbit, Mouse, Dog, Bovine, Rat, Horse, Human
WB
Unconjugated
100µl 0.5mg/ml  $325.00
SP2/0 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

ZHX2 / RAF Antibody (aa703-752) LS-C170180
Rabbit Polyclonal to Mouse ZHX2 / RAF
Mouse, Human
WB
Unconjugated
100µl 0.5mg/ml  $365.00
 This image was taken for the unconjugated form of this product. Other forms have not been tested.

ZHX2 / RAF Antibody (aa595-837, clone OTI9A5) LS-C799891
Mouse Monoclonal [clone OTI9A5] (IgG1) to Human ZHX2 / RAF
Human
WB
Unconjugated
100µl 1mg/ml  $345.00
HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY ZHX2. (Right lane) cDNA for 48 hrs and lysed

ZHX2 / RAF Antibody (aa595-837, clone OTI9A5, Carrier-free) LS-C792554
Mouse Monoclonal [clone OTI9A5] (IgG1) to Human ZHX2 / RAF
Human
WB
Unconjugated, Carrier-free
100µg  $395.00
HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY ZHX2. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZHX2. (1:2000)

ZHX2 / RAF Antibody (aa595-837, clone OTI2E2) LS-C799892
Mouse Monoclonal [clone OTI2E2] (IgG1) to Human ZHX2 / RAF
Human
WB
Unconjugated
100µl 1mg/ml  $345.00
HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY ZHX2. (Right lane) cDNA for 48 hrs and lysed

ZHX2 / RAF Antibody (aa595-837, clone OTI2E2, Carrier-free) LS-C798835
Mouse Monoclonal [clone OTI2E2] (IgG1) to Human ZHX2 / RAF
Human
WB
Unconjugated, Carrier-free
100µg  $395.00
HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY ZHX2. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZHX2. (1:2000)

ZHX2 / RAF Antibody LS-C411881
Rabbit Polyclonal to Human ZHX2 / RAF
Mouse, Rat, Human
WB
Unconjugated
100µl 1mg/ml  $385.00
Western blot of ZHX2 antibody at 1:500 dilution. Lane 1: HEK293T whole cell lysate. Lane 2: Raw264.7 whole cell lysate. Lane 3: H9C2 whole cell lysate.

ZHX2 / RAF Antibody (N-Terminus) LS-C31434
Rabbit Polyclonal (IgG) to Human ZHX2 / RAF
Rabbit, Dog, Rat, Human, Monkey
WB
Unconjugated
100µl 0.5mg/ml  $325.00
A: Marker, B: Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Showing 1-25 of 36 results

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PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).