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Antibodies > ZAK / MLTK > LS-C662967
 
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Catalog Number Size Price
LS-C662967-10 10 µg $318 
LS-C662967-100 100 µg $470 
ZAK / MLTK Antibody - Western blot analysis of MAP3K20 using anti-MAP3K20 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human PANC-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP3K20 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MAP3K20 at approximately 91KD. The expected band size for MAP3K20 is at 91KD.
ZAK / MLTK Antibody - Western blot analysis of MAP3K20 using anti-MAP3K20 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP3K20 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MAP3K20 at approximately 91KD. The expected band size for MAP3K20 is at 91KD.
ZAK / MLTK Antibody - Western blot analysis of MAP3K20 using anti-MAP3K20 antibody
ZAK / MLTK Antibody - Western blot analysis of MAP3K20 using anti-MAP3K20 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human PANC-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP3K20 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MAP3K20 at approximately 91KD. The expected band size for MAP3K20 is at 91KD.
ZAK / MLTK Antibody - Western blot analysis of MAP3K20 using anti-MAP3K20 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP3K20 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MAP3K20 at approximately 91KD. The expected band size for MAP3K20 is at 91KD.
ZAK / MLTK Antibody - Western blot analysis of MAP3K20 using anti-MAP3K20 antibody
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Polyclonal Rabbit anti‑Human ZAK / MLTK Antibody (WB) LS‑C662967

Polyclonal Rabbit anti‑Human ZAK / MLTK Antibody (WB) LS‑C662967

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Antibody:
ZAK / MLTK Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662967-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
ZAK / MLTK Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
MLTK antibody LS-C662967 is an unconjugated rabbit polyclonal antibody to human MLTK (ZAK). Validated for ELISA and WB.
Target
Human ZAK / MLTK
Synonyms
ZAK | HCCS-4 | MLTK | MLK-related kinase | MLK7 | MLT | MRK | Mlklak | AZK | Mixed lineage kinase 7 | PK
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Modifications
Unmodified
Immunogen
E. coli-derived human MAP3K20 recombinant protein (Position: F276-E511).
Specificity
Ubiquitously expressed. Isoform 2 is the predominant form in all tissues examined, except for liver, in which isoform 1 is more highly expressed.
Applications
  • Western blot
  • ELISA
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ZAK / MLTK
Q9NYL2 AB049733 NP_057737.2

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ZAK / MLTK Antibody - Immunofluorescence analysis of HepG2 cells, using MLTK Antibody. The picture on the right is blocked with the synthesized peptide.
ZAK / MLTK Antibody (aa701‑750) LS‑C119288
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Applications: Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
ZAK / MLTK Antibody - Immunohistochemistry of paraffin-embedded mouse esophageal cancer tissue.
ZAK / MLTK Antibody LS‑C349093
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot
ZAK / MLTK Antibody - Immunofluorescent analysis of ZAK staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
ZAK / MLTK Antibody (C‑Terminus) LS‑C354181
Species: Human, Mouse
Applications: ICC, Immunofluorescence, Western blot
ZAK / MLTK Antibody - Western blot analysis of extracts of various cell lines, using ZAK antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
ZAK / MLTK Antibody LS‑C749515
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Applications: Western blot
See all ZAK / MLTK Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 5/8/2024
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