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ZAK / MLTK

sterile alpha motif and leucine zipper containing kinase AZK

ZAK is a MLK(MAP3K) type protein kinase that mediates the cell cycle signals leading to G2 arrest, possibly by regulating cyclin E expression. ZAK is activated in response to osmotic shock with hyperosmolar media through autophosphorylation. The expression of ZAK is increased by activation of the ERK/MAPK pathway and activates the ERK, JNK/SAPK, p38, and ERK5 pathways. There are two alternatively spliced forms of ZAK, and only the alpha form causes the disruption of actin stress fibers and dramatic morphological changes.

Gene Name: sterile alpha motif and leucine zipper containing kinase AZK
Family/Subfamily: Protein Kinase , MLK(MAP3K)
Synonyms: ZAK, HCCS-4, MLTK, MLK-related kinase, MLK7, MLT, MRK, Mlklak, AZK, Mixed lineage kinase 7, PK
Target Sequences: AB049733 NP_057737.2 Q9NYL2

☰ Filters
Antibodies (28)
Proteins (10)
ELISA Kits (2)
Competitive EIA (2)
Over-Expression Lysate (6)
Primary (28)
Recombinant (4)
ZAK / MLTK (40)
Human (28)
Mouse (13)
Rat (4)
IHC (14)
IHC-P (3)
WB (25)
ELISA (13)
ICC (1)
IF (8)
IP (2)
Peptide-ELISA (3)
rabbit (27)
mouse (1)
Product Group
IHCPlus (1)
IgG (21)
IgG3,l (1)
monoclonal mc (1)
polyclonal pc (27)
3G5 (1)
96-Well Strip Plate (2)
APC Conjugated (1)
Azide-free (1)
Biotin Conjugated (1)
FITC Conjugated (1)
HRP Conjugated (1)
PE Conjugated (1)
Unconjugated (23)
aa271-300 (7)
aa750-800 (2)
670-750 aa, C-terminal (1)
C-Terminus (1)
aa131-180 (1)
aa700-750 (1)
aa701-750 (1)
p165 (1)
No (40)
Plasma (2)
Serum (2)
GST (1)
GST, N-terminus (1)
Myc-DDK (Flag) (8)
Human (7)
293T Cells (3)
HEK 293 Cells (5)
Wheat Germ Extract (2)
Purified (1)
ZAK / MLTK Antibody - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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ZAK / MLTK Rabbit anti-Human Polyclonal (aa271-300) Antibody
Human
IHC, IHC-P, WB
Unconjugated
400 µl/$393
ZAK / MLTK Antibody - Immunofluorescent analysis of ZAK staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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ZAK / MLTK Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Mouse, Human
ICC, IF, WB
Unconjugated
100 µl/$299
ZAK / MLTK Antibody - ZAK monoclonal antibody (M03), clone 3G5. Western blot of ZAK expression in human stomach.
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ZAK / MLTK Mouse anti-Human Monoclonal (3G5) Antibody
Human
ELISA, WB
Unconjugated
100 µg/$423
ZAK / MLTK Antibody - Immunohistochemistry of paraffin-embedded human colon carcinoma tissue.
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ZAK / MLTK Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, IHC, WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
ZAK / MLTK Antibody - Immunofluorescence analysis of HepG2 cells, using MLTK Antibody. The picture on the right is blocked with the synthesized peptide.
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ZAK / MLTK Rabbit anti-Human Polyclonal (aa701-750) Antibody
Mouse, Human
IF, Peptide-ELISA, WB
Unconjugated
50 µl/$334; 100 µl/$397
ZAK / MLTK Antibody - Anti-ZAK antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
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ZAK / MLTK Rabbit anti-Human Polyclonal Antibody
Human
IHC, IHC-P, WB
Unconjugated
50 µl/$460
ZAK / MLTK Antibody - Western blot analysis of extracts of various cell lines, using ZAK antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
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ZAK / MLTK Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
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ZAK / MLTK Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF, IHC, WB
Unconjugated
100 µg/$341
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ZAK / MLTK Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, IHC, WB
Unconjugated
60 µl/$331; 120 µl/$403; 200 µl/$529
ZAK / MLTK Antibody - Western blot of MLTK antibody
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ZAK / MLTK Rabbit anti-Human Polyclonal (670-750 aa, C-terminal) Antibody
Mouse, Human
ELISA, IF, WB
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
Select
ZAK / MLTK Rabbit anti-Human Polyclonal (aa271-300) Antibody
Human
ELISA, IHC, WB
Unconjugated
200 µl/$734
Select
ZAK / MLTK Rabbit anti-Human Polyclonal (aa271-300) (Biotin) Antibody
Human
ELISA, IHC, WB
Biotin Conjugated
200 µl/$952
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ZAK / MLTK Rabbit anti-Human Polyclonal (aa271-300) (FITC) Antibody
Human
ELISA, IHC, WB
FITC Conjugated
200 µl/$952
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ZAK / MLTK Rabbit anti-Human Polyclonal (aa271-300) (Azide-free) (HRP) Antibody
Human
ELISA, IHC, WB
HRP Conjugated, Azide-free
200 µl/$952
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ZAK / MLTK Rabbit anti-Human Polyclonal (aa271-300) (APC) Antibody
Human
ELISA, IHC, WB
APC Conjugated
200 µl/$952
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ZAK / MLTK Rabbit anti-Human Polyclonal (aa271-300) (PE) Antibody
Human
ELISA, IHC, WB
PE Conjugated
200 µl/$952
ZAK / MLTK Antibody - Peptide - + Immunofluorescence analysis of HepG2 cells, using MLTK antibody.
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ZAK / MLTK Rabbit anti-Human Polyclonal Antibody
Mouse, Human
ELISA, IF, WB
Unconjugated
100 µg/$357
ZAK / MLTK Antibody - Staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
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ZAK / MLTK Rabbit anti-Human Polyclonal Antibody
Mouse, Human
IF, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
ZAK / MLTK Antibody - Western blot analysis of MAP3K20 using anti-MAP3K20 antibody
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ZAK / MLTK Rabbit anti-Human Polyclonal Antibody
Human
ELISA, WB
Unconjugated
10 µg/$318; 100 µg/$470
ZAK / MLTK Antibody - Immunohistochemistry of paraffin-embedded Human liver cancer tissue  using ZAK  Polyclonal Antibody at dilution of 1:100(×200)
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ZAK / MLTK Rabbit anti-Human Polyclonal Antibody
Mouse, Human
ELISA, IHC
Unconjugated
20 µl/$254; 60 µl/$296; 120 µl/$355; 200 µl/$450
ZAK / MLTK Antibody - Detection of Human ZAK by Immunohistochemistry. Sample: FFPE section of human skin carcinoma. Antibody: Affinity purified rabbit anti-ZAK used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.
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ZAK / MLTK Rabbit anti-Human Polyclonal (aa750-800) Antibody
Human
IHC, IP
Unconjugated
10 µg/$295; 100 µl/$514
ZAK / MLTK Antibody - Detection of Human ZAK by Immunohistochemistry. Sample: FFPE section of human cervical carcinoma. Antibody: Affinity purified rabbit anti-ZAK used at a dilution of 1:250.
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ZAK / MLTK Rabbit anti-Human Polyclonal (aa750-800) Antibody
Human
IHC, IHC-P
Unconjugated
2.5 µg/$295; 100 µl/$532
ZAK / MLTK Antibody - Western blot analysis of extracts from Jurkat cells, using ZAK (Ab-165) antibody.
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ZAK / MLTK Rabbit anti-Human Polyclonal (p165) Antibody
Mouse, Human
ELISA, WB
Unconjugated
100 µg/$357
Select
ZAK / MLTK Rabbit anti-Human Polyclonal (aa700-750) Antibody
Human
IP, WB
Unconjugated
10 µl/$295; 100 µl/$514
ZAK / MLTK Antibody - Western blot analysis of lysates from Jurkat cells, using ZAK Antibody. The lane on the right is blocked with the synthesized peptide.
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ZAK / MLTK Rabbit anti-Human Polyclonal (aa131-180) Antibody
Mouse, Human
Peptide-ELISA, WB
Unconjugated
50 µl/$334; 100 µl/$397
Viewing 1-25 of 40 product results



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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).