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Catalog Number Size Price
LS-C407677-10 10 µg $318 
LS-C407677-100 100 µg $470 
XBP1 Antibody - XBP antibody IHC-paraffin. IHC(P): Human Tonsil Tissue.
XBP1 Antibody - XBP antibody IHC-paraffin. IHC(P): Rat Lung Tissue.
XBP1 Antibody - XBP antibody IHC-paraffin. IHC(P): Mouse Lung Tissue.
XBP1 Antibody - XBP antibody Western blot. All lanes: Anti XBP1 at 0.5 ug/ml. Lane 1: MCF-7 Whole Cell Lysate at 40 ug. Lane 2: MM231 Whole Cell Lysate at 40 ug. Lane 3: MM453 Whole Cell Lysate at 40 ug. Lane 4: SKOV Whole Cell Lysate at 40 ug. Lane 5: HELA Whole Cell Lysate at 40 ug. Predicted band size: 29 kD. Observed band size: 29 kD.
XBP1 Antibody - Flow Cytometry analysis of HepG2 cells using anti-XBP1 antibody. Overlay histogram showing HepG2 cells stained with anti-XBP1 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-XBP1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
XBP1 Antibody - XBP antibody IHC-paraffin. IHC(P): Human Tonsil Tissue.
XBP1 Antibody - XBP antibody IHC-paraffin. IHC(P): Rat Lung Tissue.
XBP1 Antibody - XBP antibody IHC-paraffin. IHC(P): Mouse Lung Tissue.
XBP1 Antibody - XBP antibody Western blot. All lanes: Anti XBP1 at 0.5 ug/ml. Lane 1: MCF-7 Whole Cell Lysate at 40 ug. Lane 2: MM231 Whole Cell Lysate at 40 ug. Lane 3: MM453 Whole Cell Lysate at 40 ug. Lane 4: SKOV Whole Cell Lysate at 40 ug. Lane 5: HELA Whole Cell Lysate at 40 ug. Predicted band size: 29 kD. Observed band size: 29 kD.
XBP1 Antibody - Flow Cytometry analysis of HepG2 cells using anti-XBP1 antibody. Overlay histogram showing HepG2 cells stained with anti-XBP1 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-XBP1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human XBP1 Antibody (aa59‑85, IHC, WB) LS‑C407677

Polyclonal Rabbit anti‑Human XBP1 Antibody (aa59‑85, IHC, WB) LS‑C407677

Antibody:
XBP1 Rabbit anti-Human Polyclonal (aa59-85) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat, Hamster
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C407677-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
XBP1 Rabbit anti-Human Polyclonal (aa59-85) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat, Hamster
Format:
Unconjugated, Unmodified

Specifications

Description
XBP1 antibody LS-C407677 is an unconjugated rabbit polyclonal antibody to XBP1 (aa59-85) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB.
Target
Human XBP1
Synonyms
XBP1 | X-box binding protein 1 | X-box-binding protein 1 | XBP2 | TREB5 | XBP-1
Host
Rabbit
Reactivity
Human, Mouse, Rat, Hamster (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the N-Terminus of human XBP1 (59-85 aa RKRQRLTHLSPEEKALRRKLKNRVAAQ), identical to the related mouse and rat sequences.
Epitope
aa59-85
Specificity
Expressed in plasma cells in rheumatoid synovium (PubMed:11460154). Over-expressed in primary breast cancer and metastatic breast cancer cells (PubMed:25280941). Isoform 1 and isoform 2 are expressed at higher level in proliferating as compared to confluent quiescent endothelial cells (PubMed:19416856). .
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
WB: The detection limit for XBP1 is approximately 0.1 ng/lane under reducing conditions. IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About XBP1
P17861 NM_005080 NP_005071.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 4/26/2024