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| Catalog Number | Size | Price |
|---|---|---|
| LS-C759832-7 | 7 ml | $274 |
Monoclonal Mouse anti‑Human Vimentin Antibody (Ready‑to‑Use, IHC) LS‑C759832
Monoclonal Mouse anti‑Human Vimentin Antibody (Ready‑to‑Use, IHC) LS‑C759832
Antibody:
Vimentin Mouse anti-Human Monoclonal (Ready-to-Use) Antibody
Application:
IHC, IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Ready-to-Use
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
Vimentin Mouse anti-Human Monoclonal (Ready-to-Use) Antibody
Application:
IHC, IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Ready-to-Use
Specifications
Description
Vimentin antibody LS-C759832 is an unconjugated mouse monoclonal antibody to human Vimentin. Validated for ELISA and IHC.
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Ready-to-Use
Immunogen
Recombinant human vimentin
Applications
- IHC (1:100 - 1:200)
- IHC - Paraffin (1:50)
- ELISA (1:100 - 1:200)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Manual Use: 1. Pretreatment: Perform heat-induced epitope retrieval (HIER) at pH 9 for 10 to 30 minutes. 2. Peroxide Block: Block in peroxidase blocking solution for 5 minutes at room temperature. (Not required if using Alkaline Phosphatase System.) 3. Primary Antibody: Apply antibody directly. Incubate antibody for 10 to 30 minutes at room temperature. 4. Secondary Antibody: Incubate for 20 to 30 minutes at room temperature. 5. Substrate Development: Incubate DAB or Fast Red for 5 to 10 minutes at room temperature. 6. Counterstain: Counterstain with hematoxylin for 0.5 to 5 minutes, depending on the hematoxylin used. Rinse with distilled water and blueing solution for 30 seconds. 7. Dehydrate and apply coverslip.
Presentation
Tris, pH 7.3-7.7, 1% BSA, <0.1% Sodium Azide
Storage
Store at 2°C to 8°C. Do not freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
About Vimentin
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Mouse, Rat, Chicken, Porcine
Applications:
IHC, IHC - Paraffin, ICC, Western blot, Immunoprecipitation
Species:
Dog, Human, Monkey, Mouse, Rat, Goat, Pig, Chicken, Zebrafish
Applications:
IHC, IHC - Frozen, ICC, Flow Cytometry
Species:
Human, Mouse, Rat, Dog
Applications:
IHC, IHC - Paraffin
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Rat
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 5/10/2024
Date Requested: 5/10/2024
