Human TSPAN7 / CD231
(tested or 100% immunogen sequence identity)
- Immunofluorescence (1:50 - 1:200)
- Western blot (1:500 - 1:2000)
TSPAN7 / CD231 antibody was raised against recombinant full length protein of human CD231
Recognizes endogenous levels of CD231 protein.
Western blot analysis of CD231 expression in rat liver (A) whole cell lysates. Immunofluorescent analysis of CD231 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
For research use only.