TRPC6 antibody LS-C109996 is an unconjugated rabbit polyclonal antibody to TRPC6 from human, mouse, rat and other species. Validated for WB.
Human, Mouse, Rat, Pig
(tested or 100% immunogen sequence identity)
Also available conjugated with Biotin
- Western blot (0.2 - 1 µg/ml)
- (applications tested for the base form of this product only)
TRPC6 antibody was raised against synthetic peptide located between aa792-841 of human TRPC6 (Q9Y210, NP_004612). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Elephant, Pig (100%); Horse, Guinea pig (92%); Marmoset (91%); Mouse (84%); Rat (76%).
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
For research use only.