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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TRAF3 Antibody (N‑Terminus) IHC‑plus™ LS‑B1428 Best Seller
Note: This antibody replaces LS-C19531
TRAF3 antibody LS-B1428 is an unconjugated rabbit polyclonal antibody to TRAF3 from human, mouse and rat. Validated for ICC, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B1428-50
50 µg (1 mg/ml)
$425

Popular TRAF3 Products

Anti-TRAF3 antibody IHC of human brain. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B609 concentration 5 ug/ml.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
Anti-TRAF3 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6511 concentration 10 ug/ml.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
Western blot of TRAF3 in various tumor cell lines using LS-C148300 at 1:2000. TRAF3 is observed at ~67 kD.
Species: Human, Mouse, Rat, Gerbil
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Immunofluorescent analysis of TRAF3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Species: Human, Mouse, Rat, Pig, Sheep
Applications: ICC, Immunofluorescence, Western blot

Product Description

TRAF3 antibody LS-B1428 is an unconjugated rabbit polyclonal antibody to TRAF3 from human, mouse and rat. Validated for ICC, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About TRAF3
Regulates pathways leading to the activation of NF-kappa-B and MAP kinases, and plays a central role in the regulation of B-cell survival. Part of signaling pathways leading to the production of cytokines and interferon. Required for normal antibody isotype switching from IgM to IgG. Plays a role T-cell dependent immune responses. Plays a role in the regulation of antiviral responses. Is an essential constituent of several E3 ubiquitin-protein ligase complexes. Q13114 NM_145725 NP_663777.1

TRAF3 Antibody, CAP-1 Antibody, CD40 associated protein 1 Antibody, CD40 binding protein Antibody, CRAF1 Antibody, LMP1 Antibody, LMP1-associated protein 1 Antibody, IIAE5 Antibody, CD40-binding protein Antibody, CD40bp Antibody, LAP1 Antibody


Specifications

Target
Human TRAF3
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • ICC
  • Western blot (2 - 4 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E30172 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-B1428. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
N-Terminus
Usage
Immunohistochemistry: LS-B1428 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1428 was determined to be 5 ug/ml.
Presentation
PBS, 0.02% Sodium Azide
Storage
Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Immunohistochemistry

Anti-TRAF3 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1428 concentration 5 ug/ml.
Anti-TRAF3 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1428 concentration 5 ug/ml.

Immunohistochemistry

Anti-TRAF3 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1428 concentration 5 ug/ml.
Anti-TRAF3 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1428 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of TRAF3 in HeLa cells with TRAF3 antibody at 10 µg/mL.
Immunocytochemistry of TRAF3 in HeLa cells with TRAF3 antibody at 10 µg/mL.

Western blot

Western blot of TRAF3 in HeLa cell lysate with TRAF3 antibody at (A) 2 and (B) 4 ug/ml.
Western blot of TRAF3 in HeLa cell lysate with TRAF3 antibody at (A) 2 and (B) 4 ug/ml.

Immunocytochemistry

Immunocytochemistry of TRAF3 in HeLa cells with TRAF3 antibody at 10 ug/ml.
Immunocytochemistry of TRAF3 in HeLa cells with TRAF3 antibody at 10 ug/ml.

Immunohistochemistry

Anti-TRAF3 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1428 concentration 5 ug/ml.
Anti-TRAF3 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1428 concentration 5 ug/ml.

Immunohistochemistry

Anti-TRAF3 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1428 concentration 5 ug/ml.
Anti-TRAF3 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1428 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of TRAF3 in HeLa cells with TRAF3 antibody at 10 µg/mL.
Immunocytochemistry of TRAF3 in HeLa cells with TRAF3 antibody at 10 µg/mL.

Western blot

Western blot of TRAF3 in HeLa cell lysate with TRAF3 antibody at (A) 2 and (B) 4 ug/ml.
Western blot of TRAF3 in HeLa cell lysate with TRAF3 antibody at (A) 2 and (B) 4 ug/ml.

Immunocytochemistry

Immunocytochemistry of TRAF3 in HeLa cells with TRAF3 antibody at 10 ug/ml.
Immunocytochemistry of TRAF3 in HeLa cells with TRAF3 antibody at 10 ug/ml.

Immunohistochemistry

Anti-TRAF3 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1428 concentration 5 ug/ml.
Anti-TRAF3 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1428 concentration 5 ug/ml.

Immunohistochemistry

Anti-TRAF3 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1428 concentration 5 ug/ml.
Anti-TRAF3 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1428 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of TRAF3 in HeLa cells with TRAF3 antibody at 10 µg/mL.
Immunocytochemistry of TRAF3 in HeLa cells with TRAF3 antibody at 10 µg/mL.

Western blot

Western blot of TRAF3 in HeLa cell lysate with TRAF3 antibody at (A) 2 and (B) 4 ug/ml.
Western blot of TRAF3 in HeLa cell lysate with TRAF3 antibody at (A) 2 and (B) 4 ug/ml.

Immunocytochemistry

Immunocytochemistry of TRAF3 in HeLa cells with TRAF3 antibody at 10 ug/ml.
Immunocytochemistry of TRAF3 in HeLa cells with TRAF3 antibody at 10 ug/ml.

Immunohistochemistry

Anti-TRAF3 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1428 concentration 5 ug/ml.
Anti-TRAF3 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1428 concentration 5 ug/ml.

Immunohistochemistry

Anti-TRAF3 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1428 concentration 5 ug/ml.
Anti-TRAF3 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1428 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of TRAF3 in HeLa cells with TRAF3 antibody at 10 µg/mL.
Immunocytochemistry of TRAF3 in HeLa cells with TRAF3 antibody at 10 µg/mL.

Western blot

Western blot of TRAF3 in HeLa cell lysate with TRAF3 antibody at (A) 2 and (B) 4 ug/ml.
Western blot of TRAF3 in HeLa cell lysate with TRAF3 antibody at (A) 2 and (B) 4 ug/ml.

Immunocytochemistry

Immunocytochemistry of TRAF3 in HeLa cells with TRAF3 antibody at 10 ug/ml.
Immunocytochemistry of TRAF3 in HeLa cells with TRAF3 antibody at 10 ug/ml.

Requested From: United States
Date Requested: 8/20/2018

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