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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TRAF3 Antibody (Internal) LS‑C352959
TRAF3 antibody LS-C352959 is an unconjugated rabbit polyclonal antibody to TRAF3 from human, mouse, rat and other species. Validated for ICC, IF and WB.
Catalog
Size
Price
LS-C352959-100
100 µl (1 mg/ml)
$245

Popular TRAF3 Products

Anti-TRAF3 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1428 concentration 5 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Western blot
Anti-TRAF3 antibody IHC of human brain. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B609 concentration 5 ug/ml.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
Anti-TRAF3 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6511 concentration 10 ug/ml.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
Western blot of TRAF3 in various tumor cell lines using LS-C148300 at 1:2000. TRAF3 is observed at ~67 kD.
Species: Human, Mouse, Rat, Gerbil
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation

Product Description

TRAF3 antibody LS-C352959 is an unconjugated rabbit polyclonal antibody to TRAF3 from human, mouse, rat and other species. Validated for ICC, IF and WB.

Specifications

Target
Human TRAF3
Synonyms
TRAF3, CAP-1, CD40 associated protein 1, CD40 binding protein, CRAF1, LMP1, LMP1-associated protein 1, IIAE5, CD40-binding protein, CD40bp, LAP1
Host
Rabbit
Reactivity
Human, Mouse, Rat, Pig, Sheep (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Immunogen
TRAF3 antibody was raised against kLH-conjugated synthetic peptide encompassing a sequence within the central region of human TRAF3.
Blocking Peptide
LS-E40925 - Lyophilized - 1 mg - $145.00
Immunizing peptide used to generate LS-C352959. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
Internal
Specificity
Recognizes endogenous levels of TRAF3 protein.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only.
About TRAF3
Regulates pathways leading to the activation of NF-kappa-B and MAP kinases, and plays a central role in the regulation of B-cell survival. Part of signaling pathways leading to the production of cytokines and interferon. Required for normal antibody isotype switching from IgM to IgG. Plays a role T-cell dependent immune responses. Plays a role in the regulation of antiviral responses. Is an essential constituent of several E3 ubiquitin-protein ligase complexes. Q13114 NM_145725 NP_663777.1


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Images

Immunofluorescence

Immunofluorescent analysis of TRAF3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Immunofluorescent analysis of TRAF3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot

Western blot analysis of TRAF3 expression in MCF7 (A); HeLa (B); Raji (C); NIH3T3 (D); mouse lung (E); rat colon (F); PC12 (G) whole cell lysates.
Western blot analysis of TRAF3 expression in MCF7 (A); HeLa (B); Raji (C); NIH3T3 (D); mouse lung (E); rat colon (F); PC12 (G) whole cell lysates.

Immunofluorescence

Immunofluorescent analysis of TRAF3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Immunofluorescent analysis of TRAF3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot

Western blot analysis of TRAF3 expression in MCF7 (A); HeLa (B); Raji (C); NIH3T3 (D); mouse lung (E); rat colon (F); PC12 (G) whole cell lysates.
Western blot analysis of TRAF3 expression in MCF7 (A); HeLa (B); Raji (C); NIH3T3 (D); mouse lung (E); rat colon (F); PC12 (G) whole cell lysates.

Immunofluorescence

Immunofluorescent analysis of TRAF3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Immunofluorescent analysis of TRAF3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot

Western blot analysis of TRAF3 expression in MCF7 (A); HeLa (B); Raji (C); NIH3T3 (D); mouse lung (E); rat colon (F); PC12 (G) whole cell lysates.
Western blot analysis of TRAF3 expression in MCF7 (A); HeLa (B); Raji (C); NIH3T3 (D); mouse lung (E); rat colon (F); PC12 (G) whole cell lysates.

Immunofluorescence

Immunofluorescent analysis of TRAF3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Immunofluorescent analysis of TRAF3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot

Western blot analysis of TRAF3 expression in MCF7 (A); HeLa (B); Raji (C); NIH3T3 (D); mouse lung (E); rat colon (F); PC12 (G) whole cell lysates.
Western blot analysis of TRAF3 expression in MCF7 (A); HeLa (B); Raji (C); NIH3T3 (D); mouse lung (E); rat colon (F); PC12 (G) whole cell lysates.

Requested From: United States
Date Requested: 10/23/2018

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