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Catalog Number Size Price
LS-C758300-100 100 µg (0.5 mg/ml) $503 
TNFSF11 / RANKL / TRANCE Antibody - Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and secreted staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
TNFSF11 / RANKL / TRANCE Antibody - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and secreted staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
TNFSF11 / RANKL / TRANCE Antibody - Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
TNFSF11 / RANKL / TRANCE Antibody - Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and secreted staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
TNFSF11 / RANKL / TRANCE Antibody - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and secreted staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
TNFSF11 / RANKL / TRANCE Antibody - Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Polyclonal Goat anti‑Human TNFSF11 / RANKL / TRANCE Antibody (IF) LS‑C758300

Polyclonal Goat anti‑Human TNFSF11 / RANKL / TRANCE Antibody (IF) LS‑C758300

Antibody:
TNFSF11 / RANKL / TRANCE Goat anti-Human Polyclonal Antibody
Application:
IF, Peptide-ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$503
LS-C758300-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
TNFSF11 / RANKL / TRANCE Goat anti-Human Polyclonal Antibody
Application:
IF, Peptide-ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
TRANCE antibody LS-C758300 is an unconjugated goat polyclonal antibody to TRANCE (TNFSF11 / RANKL) from human. It is reactive with human and mouse. Validated for IF and Peptide-ELISA.
Target
Human TNFSF11 / RANKL / TRANCE
Synonyms
TNFSF11 | CD254 | CD254 antigen | HRANKL2 | OPTB2 | TRANCE | ODF | OPGL | SOdf | Osteoprotegerin ligand | RANKL
Host
Goat
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-DLAKRSKLEAQP, from the internal region of the protein sequence according to NP_003692.1NP_143026.1.
Specificity
This antibody is expected to recognise both reported isoforms (NP_003692.1 and NP_143026.1).
Applications
  • Immunofluorescence (10 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Usage
Peptide ELISA: antibody detection limit dilution 1:16000. Immunofluorescence: Strong expression of the protein seen in the membranes of MCF7 and NIH3T3 cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TNFSF11 / RANKL / TRANCE
O14788 AF053712 AAC39731.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/2/2024