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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TNFSF11 / RANKL / TRANCE

tumor necrosis factor (ligand) superfamily, member 11

TNFSF11 / RANKL / TRANCE is a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found.

Gene Name: tumor necrosis factor (ligand) superfamily, member 11
Family/Subfamily: TNF , not assigned-TNF
Synonyms: TNFSF11, CD254, CD254 antigen, HRANKL2, OPTB2, TRANCE, ODF, OPGL, SOdf, Osteoprotegerin ligand, RANKL
Target Sequences: AF053712 AAC39731.1 O14788

Publications (3)

1
Progestin-regulated luminal cell and myoepithelial cell-specific responses in mammary organoid culture. Haslam SZ, Drolet A, Smith K, Tan M, Aupperlee M. Endocrinology. 2008 149:2098-107. [PubMed:18218689] [PMC:PMC2329279] Related Antibodies: LS-C41357.
2
Early dissemination seeds metastasis in breast cancer. Hosseini H, Obradovic MM, Hoffmann M, Harper KL, Sosa MS, Werner-Klein M, Nanduri LK, Werno C, Ehrl C, Maneck M, Patwary N, Haunschild G,Gužvic M, Reimelt C, Grauvogl M, Eichner N, Weber F, Hartkopf AD, Taran FA, Brucker SY, Fehm T, Rack B, Buchholz S, Spang R, Meister G, Aguirre-Ghiso JA, Klein CA. Nature. 2016 (WB; Mouse) [Full Text Article] [PubMed:27974799] [PMC:PMC5390864]
3
Transient gamma-secretase inhibition accelerates and enhances fracture repair likely via Notch signaling modulation. Wang C, Shen J, Yukata K, Inzana JA, O'Keefe RJ, Awad HA, Hilton MJ. Bone. Apr;73:77-89. (IHC; Mouse) [Full Text Article] [PubMed:25527421] [PMC:PMC4336841] Related Antibodies: LS-B1425.

Filtered by: None
Target
TNFSF11 / RANKL / TRANCE (158)
Reactivity
Human (100)
Mouse (64)
Rat (43)
Bovine (2)
Application
IHC (77)
IHC-Fr (4)
IHC-P (9)
WB (140)
Flo (20)
ELISA (60)
ICC (8)
IF (11)
IP (9)
Peptide-ELISA (2)
Host
rabbit (91)
mouse (45)
goat (9)
rat (13)
Isotype
IgG (51)
IgG1 (8)
IgG1,k (13)
IgG2a (8)
IgG2a,k (27)
IgG3 (1)
Clonality
monoclonal mc (58)
polyclonal pc (100)
Clone
12A380 (3)
12A668 (2)
3H8A7 (1)
4H9 (1)
7G3D7 (1)
8A7B9 (1)
IK22/5 (5)
TS106 (1)
Format
AF488 Conjugated (1)
AF647 Conjugated (1)
AP Conjugated (5)
APC (7)
APC Conjugated (11)
Azide-free (3)
Biotin Conjugated (21)
Cy3 Conjugated (7)
Cy7 Conjugated (7)
FITC Conjugated (18)
HRP Conjugated (15)
PE Conjugated (13)
Unconjugated (59)
Epitope
aa106-240 (10)
aa79-247 (10)
aa92-263 (10)
aa1-30 (8)
aa1-317 (8)
10-90 aa, Internal (1)
261-310 aa, C-terminal (1)
Ala72 (1)
Internal (1)
aa1-45 (1)
aa143-316 (1)
aa151-316 (1)
aa157-316 (1)
aa161-177 (1)
aa253-317 (1)
aa41-90 (1)
aa64-245 (1)
Publications
No (156)
Yes (2)
Showing 1-25 of 158 results

TNFSF11 / RANKL / TRANCE Antibody IHC-plus™ LS-B1425
Publications (1)
Publications (1)
Rabbit Polyclonal (IgG) to Human TNFSF11 / RANKL / TRANCE
Mouse, Rat, Human
ELISA IHC IHC-P WB
Unconjugated
0.05mg  $425.00
Anti-RANKL antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-RANKL antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

TNFSF11 / RANKL / TRANCE Antibody (clone IK22/5) LS-C41357
Publications (1)
Publications (1)
Rat Monoclonal [clone IK22/5] (IgG2a) to Mouse TNFSF11 / RANKL / TRANCE
Mouse
Flo IP WB
Unconjugated
50µg  $245.00
Mouse TRANCE transfected cells stained with purified IK22-5, followed by biotinylated anti-rat IgG and Sav-PE

TNFSF11 / RANKL / TRANCE Antibody (Biotin) LS-C86684
Goat Polyclonal to Human TNFSF11 / RANKL / TRANCE
Human
ELISA WB
Biotin Conjugated
50µg  $435.00

TNFSF11 / RANKL / TRANCE Antibody (aa1-317, Azide-free) LS-C19335
Mouse Monoclonal (IgG1,k) to Mouse TNFSF11 / RANKL / TRANCE
Mouse, Human
IHC IHC-P WB
Unconjugated, Azide-free
100µg 1mg/ml  $335.00
Western blot of TRANCE/RANKL in transfected cells. A single protein band of approximate mol. wt. of 35 kD was detected. This image was taken for the unmodified form of this product. Other forms have not been tested.

TNFSF11 / RANKL / TRANCE Antibody LS-C104835
Mouse Monoclonal (IgG1) to Human TNFSF11 / RANKL / TRANCE
Human
ELISA
Unconjugated
500µg  $255.00
Sandwich ELISA of RANKL antibody

TNFSF11 / RANKL / TRANCE Antibody (aa1-30) LS-C160070
Rabbit Polyclonal to Human TNFSF11 / RANKL / TRANCE
Human
Flo IHC IHC-P WB
Unconjugated
400µl  $325.00
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with TNFSF11 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of TNFSF11 Antibody in HL-60 cell line lysates (35 ug/lane). TNFSF11 (arrow) was detected using the purified antibody.

TNFSF11 / RANKL / TRANCE Antibody LS-C332159
Rabbit Polyclonal (IgG) to Human TNFSF11 / RANKL / TRANCE
Mouse, Human
IF IHC WB
Unconjugated
50µl  $235.00; 100µl  $315.00; 200µl  $425.00
Immunohistochemistry of paraffin-embedded rat Intestine.
Immunohistochemistry of paraffin-embedded rat kidney tissue.

TNFSF11 / RANKL / TRANCE Antibody LS-C104767
Rabbit Polyclonal to Mouse TNFSF11 / RANKL / TRANCE
Mouse
ELISA WB
Unconjugated
50µg  $285.00; 100µg  $325.00
Western Blot (non-reducing) of RANKL antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (reducing) of RANKL antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

TNFSF11 / RANKL / TRANCE Antibody (aa41-90) LS-C118662
Rabbit Polyclonal (IgG) to Human TNFSF11 / RANKL / TRANCE
Mouse, Human
IF Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunofluorescence analysis of HepG2 cells, using TNFSF11 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from HUVEC cells, using TNFSF11 Antibody. The lane on the right is blocked with the synthesized peptide.

TNFSF11 / RANKL / TRANCE Antibody IHC-plus™ LS-B7418
Rabbit Polyclonal (IgG) to Human TNFSF11 / RANKL / TRANCE
Human
ELISA IHC IHC-P WB
Unconjugated
50µg  $425.00
Anti-RANKL antibody IHC of human tonsil, germinal center. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Western Blot (non-reducing) of RANKL antibody

TNFSF11 / RANKL / TRANCE Antibody (Biotin) LS-C104393
Rabbit Polyclonal to Human TNFSF11 / RANKL / TRANCE
Human
ELISA WB
Biotin Conjugated
25µg  $285.00; 50µg  $325.00
Biotinylated Anti-Human sRANK Ligand (Polyclonal Rabbit) Western Blot Unreduced
Biotinylated Anti-Human sRANK Ligand (Polyclonal Rabbit) Western Blot Reduced

TNFSF11 / RANKL / TRANCE Antibody (clone 8A7B9) LS-C682548
Mouse Monoclonal [clone 8A7B9] (IgG1) to Human TNFSF11 / RANKL / TRANCE
Human
ELISA Flo ICC IHC WB
Unconjugated
100µl 1mg/ml  $365.00

TNFSF11 / RANKL / TRANCE Antibody (aa161-177) LS-C829
Rabbit Polyclonal (IgG) to Mouse TNFSF11 / RANKL / TRANCE
Mouse
WB
Unconjugated
100µg 200µl 0.5mg/ml  $335.00
Western blot of Trance in a partial His-tagged recombinant protein using antibody at0.5 ug/ml.

TNFSF11 / RANKL / TRANCE Antibody (aa64-245, clone TS106) LS-C191486
Mouse Monoclonal [clone TS106] (IgG3) to Human TNFSF11 / RANKL / TRANCE
Human
WB
Unconjugated
100µg  $650.00

TNFSF11 / RANKL / TRANCE Antibody IHC-plus™ LS-B7419
Goat Polyclonal (IgG) to Human TNFSF11 / RANKL / TRANCE
Human
ELISA IHC IHC-P WB
Unconjugated
50µg  $425.00
Anti-RANKL antibody IHC of human tonsil, germinal center. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Western Blot (non-reducing) of RANKL antibody

TNFSF11 / RANKL / TRANCE Antibody (Biotin) LS-C104397
Goat Polyclonal to Human TNFSF11 / RANKL / TRANCE
Human
ELISA WB
Biotin Conjugated
25µg  $285.00; 50µg  $325.00

TNFSF11 / RANKL / TRANCE Antibody (aa1-317, clone 12A668, Biotin) LS-C147977
Mouse Monoclonal [clone 12A668] (IgG1,k) to Mouse TNFSF11 / RANKL / TRANCE
Mouse, Human
ELISA
Biotin Conjugated
100µg 0.5mg/ml  $365.00

TNFSF11 / RANKL / TRANCE Antibody (Internal) LS-C358364
Rabbit Polyclonal to Human TNFSF11 / RANKL / TRANCE
Human
ICC IF IHC IHC-P WB
Unconjugated
100µl 1mg/ml  $245.00
Immunohistochemical analysis of CD254 staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of CD254 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

TNFSF11 / RANKL / TRANCE Antibody (FITC) LS-C211871
Rabbit Polyclonal (IgG) to Mouse TNFSF11 / RANKL / TRANCE
ELISA
FITC Conjugated
50µg  $285.00; 100µg 0.25mg/ml  $385.00

TNFSF11 / RANKL / TRANCE Antibody (clone 3H8A7) LS-C682549
Mouse Monoclonal [clone 3H8A7] (IgG1) to Human TNFSF11 / RANKL / TRANCE
Human
ELISA Flo IHC WB
Unconjugated
100µl 1mg/ml  $365.00

TNFSF11 / RANKL / TRANCE Antibody (aa1-317) LS-C17589
Mouse Monoclonal (IgG1) to Mouse TNFSF11 / RANKL / TRANCE
Mouse, Human
Flo IHC IHC-Fr WB
Unconjugated
100µg  $665.00

TNFSF11 / RANKL / TRANCE Antibody LS-C755262
Rabbit Polyclonal (IgG) to Human TNFSF11 / RANKL / TRANCE
Human
ELISA IF IHC WB
Unconjugated
100µg 1mg/ml  $375.00

TNFSF11 / RANKL / TRANCE Antibody (aa1-317, clone 12A380) LS-C605
Mouse Monoclonal [clone 12A380] (IgG1) to Mouse TNFSF11 / RANKL / TRANCE
Mouse, Human
Flo IHC IHC-Fr WB
Unconjugated
100µg 200µl 0.5mg/ml  $335.00
Western blot of TRANCE in transfected cells using antibody at 5 ug/ml.

TNFSF11 / RANKL / TRANCE Antibody LS-C393179
Rabbit Polyclonal to Human TNFSF11 / RANKL / TRANCE
Mouse, Rat, Human
IF IHC IHC-P WB
Unconjugated
100µl  $305.00

TNFSF11 / RANKL / TRANCE Antibody LS-C499950
Rabbit Polyclonal (IgG) to Human TNFSF11 / RANKL / TRANCE
Human
ELISA IF IHC
Unconjugated
50µg 1mg/ml  $285.00; 100µg 1mg/ml  $385.00
Immunohistochemistry of paraffin-embedded human gastric cancer using TNFSF11 Antibody at dilution of 1:100
Immunohistochemistry of paraffin-embedded human colon cancer using TNFSF11 Antibody at dilution of 1:100
Showing 1-25 of 158 results

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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