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Catalog Number Size Price
LS-C661946-10 10 µg $318 
LS-C661946-100 100 µg $470 
TNFRSF1A / TNFR1 Antibody - Western blot analysis of TNFRSF1A using anti-TNFRSF1A antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat gaster tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNFRSF1A antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TNFRSF1A at approximately 60KD. The expected band size for TNFRSF1A is at 50KD.
TNFRSF1A / TNFR1 Antibody - Western blot - Anti-TNFRSF1A/Tnf Ri Picoband Antibody
TNFRSF1A / TNFR1 Antibody - Western blot analysis of TNFRSF1A using anti-TNFRSF1A antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat gaster tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNFRSF1A antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TNFRSF1A at approximately 60KD. The expected band size for TNFRSF1A is at 50KD.
TNFRSF1A / TNFR1 Antibody - Western blot - Anti-TNFRSF1A/Tnf Ri Picoband Antibody
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Polyclonal Rabbit anti‑Human TNFRSF1A / TNFR1 Antibody (WB) LS‑C661946

Polyclonal Rabbit anti‑Human TNFRSF1A / TNFR1 Antibody (WB) LS‑C661946

Antibody:
TNFRSF1A / TNFR1 Rabbit anti-Human Polyclonal Antibody
Application:
WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C661946-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
TNFRSF1A / TNFR1 Rabbit anti-Human Polyclonal Antibody
Application:
WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
TNFR1 antibody LS-C661946 is an unconjugated rabbit polyclonal antibody to human TNFR1 (TNFRSF1A). Validated for WB.
Target
Human TNFRSF1A / TNFR1
Synonyms
TNFRSF1A | CD120a | CD120a antigen | MS5 | p60 | TBP1 | TNF-R55 | TNFAR | TNFR1 | p55-R | TNF-R | TNF-R-I | TNF-R1 | TNF-RI | Tnfr | TNFR-I | TNFR1-d2 | TNFR60 | FPF | p55 | Tbpi | TNFR55
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human TNFRSF1A recombinant protein (Position: F89-N145). Human TNFRSF1A shares 67.3% and 69.1% amino acid (aa) sequence identity with mouse and rat TNFRSF1A, respectively.
Applications
  • Western blot
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TNFRSF1A / TNFR1
P19438 NM_001065 NP_001056.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/27/2024