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Antibodies > Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 > LS-C829092
 
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Catalog Number Size Price
LS-C829092-40 40 µg (0.5 mg/ml) $295 
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Western Blot of HEK293 transfected with PX458 (pSpCas9(BB)-2A-GFP) or untransfected cell lysates with two independent antibodies: SpCas9 Antibody (4A1) and SpCas9 Antibody (14B6). The correlated pattern indicates the high specificity of these two antibodies. Lane 1: 50 µg HEK293 transfected with PX458 cell Lysate. Lane 2: 50 µg Untransfected HEK293 cell Lysate. Lane 3: 40 ng SpCas9 recombinant protein. Lane 4: 50 µg HEK293 transfected with PX458 cell Lysate. Lane 5: 50 µg Untransfected HEK293 cell Lysate. Lane 6: 40 ng SpCas9 recombinant protein. Primary Antibody: Lane 1~3: SpCas9 Antibody (4A1) 1 µg/ml. Lane 4~6: SpCas9 Antibody (14B6) 1 µg/ml. Secondary Antibody: Goat anti-Mouse IgG (H&L) [IRDye800] 0.125 µg/ml.
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Western Blot of recombinant Streptococcus pyogenes CRISPR/Cas9 protein with two independent antibodies: SpCas9 Antibody (4A1) and SpCas9 Antibody (14B6). The correlated pattern indicates the high specificity of these two antibodies. Lane 1: 50 ng SpCas9 recombinant protein. Lane 2: 25 ng SpCas9 recombinant protein. Lane 3: 10 ng SpCas9 recombinant protein. Lane 4: 5 ng SpCas9 recombinant protein. Lane 5: 50 ng SpCas9 recombinant protein. Lane 6: 25 ng SpCas9 recombinant protein. Lane 7: 10 ng SpCas9 recombinant protein. Lane 8: 5 ng SpCas9 recombinant protein. Primary Antibody: Lane 1~4: SpCas9 Antibody (4A1) 1 µg/ml. Lane 5~8: SpCas9 Antibody (14B6) 1 µg/ml. Secondary Antibody: Goat anti-Mouse IgG (H&L) [IRDye800] 0.125 µg/ml.
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Western Blot analysis of HEK293 transfected with various plasmids with SpCas9 Antibody (14B6). The different HEK293 with transfected with various plasmids indicate the minimum cross reaction of the antibody. Loading: Lane 1: 50µg HEK293 cell lysate transfected with SaCas9(BB)-2A-GFP (J7RUA5, Staphylococcus aureus). Lane 2: 50µg HEK293 cell lysate transfected with StCas9(BB)-2A-GFP (G3ECR1, Streptococcus thermophiles). Lane 3: 50µg HEK293 cell lysate transfected with FnCpf1(BB)-2A-GFP (Francisella tularensis subsp. novicida (strain U112)). Lane 4: 50µg HEK293 cell lysate transfected with LbCpf1(BB)-2A-GFP (Lachnospiraceae bacterium ND2006). Lane 5: 50µg HEK293 cell lysate transfected with AsCpf1(BB)-2A-GFP (Acidaminococcus sp. (strain BV3L6)). Lane 6: 50µg HEK293 cell lysate transfected with pSpCas9(BB)-2A-GFP (PX458, Q99ZW2, Streptococcus pyogenes serotype M1). Lane 7: 50µg HEK293 cell lysate (Non-transfected). Primary Antibody: Lane 1~7: SpCas9 Antibody (14B6) 1 µg/ml. Lane 1~7: Anti-a-tubulin mAb (mouse)(1F4) 0.5µg/ml. Secondary antibody: Goat anti-Mouse IgG (H&L) [IRDye800] 0.125 µg/ml.
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Western Blot of HEK293 transfected with PX458 (pSpCas9(BB)-2A-GFP) or untransfected cell lysates with SpCas9 Antibody (14B6). The different concentration of antibodies indicates the high affinity and sensitivity of the antibody. Lane 1: 50 µg HEK293 transfected with PX458 cell Lysate. Lane 2: 50 µg Untransfected HEK293 cell Lysate. Lane 3: 40 ng SpCas9 recombinant protein. Lane 4: 50 µg HEK293 transfected with PX458 cell Lysate. Lane 5: 50 µg Untransfected HEK293 cell Lysate. Lane 6: 40 ng SpCas9 recombinant protein. Lane 7: 50 µg HEK293 transfected with PX458 cell Lysate. Lane 8: 50 µg Untransfected HEK293 cell Lysate. Lane 9: 40 ng SpCas9 recombinant protein. Primary Antibody: Lane 1~3: SpCas9 Antibody (14B6) 2 µg/ml. Lane 4~6: SpCas9 Antibody (14B6) 1 µg/ml. Lane 7~9: SpCas9 Antibody (14B6) 0.5 µg/ml. Secondary Antibody: Goat anti-Mouse IgG (H&L) [IRDye800] 0.125 µg/ml.
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Standard curve of SpCas9 Sandwich ELISA. The SpCas9 Sandwich ELISA assay is developed by using SpCas9 Antibody (14B6) and SpCas9 Antibody (4A1) as capture and detection antibody, respectively. These two antibodies recognize different epitopes. In this ELISA assay, SpCas9 Antibody (4A1) was labeled with Biotin. The sensitivity is <1 ng/ml and the detection range is 0-10 ng/ml.
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - ELISA binding of SpCas9 Antibody (14B6) with recombinant Streptococcus pyogenes CRISPR/Cas9 protein. Coating antigen: SpCas9, 1 ug/ml. SpCas9 antibody dilution start from 3000 ng/ml, EC50= 24.51 ng/ml.
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Western Blot of HEK293 transfected with PX458 (pSpCas9(BB)-2A-GFP) or untransfected cell lysates with two independent antibodies: SpCas9 Antibody (4A1) and SpCas9 Antibody (14B6). The correlated pattern indicates the high specificity of these two antibodies. Lane 1: 50 µg HEK293 transfected with PX458 cell Lysate. Lane 2: 50 µg Untransfected HEK293 cell Lysate. Lane 3: 40 ng SpCas9 recombinant protein. Lane 4: 50 µg HEK293 transfected with PX458 cell Lysate. Lane 5: 50 µg Untransfected HEK293 cell Lysate. Lane 6: 40 ng SpCas9 recombinant protein. Primary Antibody: Lane 1~3: SpCas9 Antibody (4A1) 1 µg/ml. Lane 4~6: SpCas9 Antibody (14B6) 1 µg/ml. Secondary Antibody: Goat anti-Mouse IgG (H&L) [IRDye800] 0.125 µg/ml.
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Western Blot of recombinant Streptococcus pyogenes CRISPR/Cas9 protein with two independent antibodies: SpCas9 Antibody (4A1) and SpCas9 Antibody (14B6). The correlated pattern indicates the high specificity of these two antibodies. Lane 1: 50 ng SpCas9 recombinant protein. Lane 2: 25 ng SpCas9 recombinant protein. Lane 3: 10 ng SpCas9 recombinant protein. Lane 4: 5 ng SpCas9 recombinant protein. Lane 5: 50 ng SpCas9 recombinant protein. Lane 6: 25 ng SpCas9 recombinant protein. Lane 7: 10 ng SpCas9 recombinant protein. Lane 8: 5 ng SpCas9 recombinant protein. Primary Antibody: Lane 1~4: SpCas9 Antibody (4A1) 1 µg/ml. Lane 5~8: SpCas9 Antibody (14B6) 1 µg/ml. Secondary Antibody: Goat anti-Mouse IgG (H&L) [IRDye800] 0.125 µg/ml.
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Western Blot analysis of HEK293 transfected with various plasmids with SpCas9 Antibody (14B6). The different HEK293 with transfected with various plasmids indicate the minimum cross reaction of the antibody. Loading: Lane 1: 50µg HEK293 cell lysate transfected with SaCas9(BB)-2A-GFP (J7RUA5, Staphylococcus aureus). Lane 2: 50µg HEK293 cell lysate transfected with StCas9(BB)-2A-GFP (G3ECR1, Streptococcus thermophiles). Lane 3: 50µg HEK293 cell lysate transfected with FnCpf1(BB)-2A-GFP (Francisella tularensis subsp. novicida (strain U112)). Lane 4: 50µg HEK293 cell lysate transfected with LbCpf1(BB)-2A-GFP (Lachnospiraceae bacterium ND2006). Lane 5: 50µg HEK293 cell lysate transfected with AsCpf1(BB)-2A-GFP (Acidaminococcus sp. (strain BV3L6)). Lane 6: 50µg HEK293 cell lysate transfected with pSpCas9(BB)-2A-GFP (PX458, Q99ZW2, Streptococcus pyogenes serotype M1). Lane 7: 50µg HEK293 cell lysate (Non-transfected). Primary Antibody: Lane 1~7: SpCas9 Antibody (14B6) 1 µg/ml. Lane 1~7: Anti-a-tubulin mAb (mouse)(1F4) 0.5µg/ml. Secondary antibody: Goat anti-Mouse IgG (H&L) [IRDye800] 0.125 µg/ml.
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Western Blot of HEK293 transfected with PX458 (pSpCas9(BB)-2A-GFP) or untransfected cell lysates with SpCas9 Antibody (14B6). The different concentration of antibodies indicates the high affinity and sensitivity of the antibody. Lane 1: 50 µg HEK293 transfected with PX458 cell Lysate. Lane 2: 50 µg Untransfected HEK293 cell Lysate. Lane 3: 40 ng SpCas9 recombinant protein. Lane 4: 50 µg HEK293 transfected with PX458 cell Lysate. Lane 5: 50 µg Untransfected HEK293 cell Lysate. Lane 6: 40 ng SpCas9 recombinant protein. Lane 7: 50 µg HEK293 transfected with PX458 cell Lysate. Lane 8: 50 µg Untransfected HEK293 cell Lysate. Lane 9: 40 ng SpCas9 recombinant protein. Primary Antibody: Lane 1~3: SpCas9 Antibody (14B6) 2 µg/ml. Lane 4~6: SpCas9 Antibody (14B6) 1 µg/ml. Lane 7~9: SpCas9 Antibody (14B6) 0.5 µg/ml. Secondary Antibody: Goat anti-Mouse IgG (H&L) [IRDye800] 0.125 µg/ml.
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Standard curve of SpCas9 Sandwich ELISA. The SpCas9 Sandwich ELISA assay is developed by using SpCas9 Antibody (14B6) and SpCas9 Antibody (4A1) as capture and detection antibody, respectively. These two antibodies recognize different epitopes. In this ELISA assay, SpCas9 Antibody (4A1) was labeled with Biotin. The sensitivity is <1 ng/ml and the detection range is 0-10 ng/ml.
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - ELISA binding of SpCas9 Antibody (14B6) with recombinant Streptococcus pyogenes CRISPR/Cas9 protein. Coating antigen: SpCas9, 1 ug/ml. SpCas9 antibody dilution start from 3000 ng/ml, EC50= 24.51 ng/ml.
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Monoclonal Mouse anti‑Streptococcus pyogenes Streptococcus pyogenes CRISPR‑associated endonuclease Cas9/Csn1 Antibody (clone 14B6, IF, WB) LS‑C829092

Monoclonal Mouse anti‑Streptococcus pyogenes Streptococcus pyogenes CRISPR‑associated endonuclease Cas9/Csn1 Antibody (clone 14B6, IF, WB) LS‑C829092

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Antibody:
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Mouse anti-Streptococcus pyogenes Monoclonal (14B6) Antibody
Application:
IF, WB, ELISA
Reactivity:
Streptococcus pyogenes
Format:
Unconjugated, Unmodified
Price
Catalog Number
$295
LS-C829092-40
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Mouse anti-Streptococcus pyogenes Monoclonal (14B6) Antibody
Application:
IF, WB, ELISA
Reactivity:
Streptococcus pyogenes
Format:
Unconjugated, Unmodified

Specifications

Description
Csn1 antibody LS-C829092 is an unconjugated mouse monoclonal antibody to streptococcus pyogenes Csn1 (Streptococcus pyogenes CRISPR-associated endonuclease Cas9). Validated for ELISA, IF and WB.
Target
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1
Host
Mouse
Reactivity
Streptococcus pyogenes (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
14B6
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Recombinant Streptococcus pyogenes CRISPR/Cas9
Specificity
The product is specific for Streptococcus pyogenes CRISPR/Cas9. This antibody binds with recombinant Streptococcus pyogenes CRISPR/Cas9 protein in ELISA and Endogenous overexpressed Streptococcus pyogenes CRISPR/Cas9 in immunofluorescence, western blot.
Applications
  • Immunofluorescence (1 - 2 µg/ml)
  • Western blot (0.5 - 2 µg/ml)
  • ELISA (0.05 - 1 µg/ml)
Usage
Applications should be user optimized.
Presentation
Lyophilized from PBS, pH 7.4, 0.02% Sodium Azide
Reconstitution
Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Storage
Store lyophilized at -20°C for up to 1 year. Store reconstituted at 2°C to 8°C for up to 3 weeks. Aliquot and store at -20°C or below for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Featured Products

Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCAS-Guide vector (GE100002, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CAS9.
Streptococcus pyogenes CRISPR‑associated endonuclease Cas9/Csn1 Antibody (aa1150‑1200) LS‑C340585
Species: Streptococcus pyogenes
Applications: Western blot
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Immunofluorescence of rabbit Anti-Cas9 Antibody. Cells: HeLa cells transfected with GFP+Cas9 or GFP+CD-3. Fixation: Formaldehyde fixed, permeabilized Triton X-100. Primary: Anti-Cas9 used at 1µg/mL. Secondary: Donkey anti-Rabbit at 1µg/mL, staining seen in red. Bottom images show GFP stain overlay.
Streptococcus pyogenes CRISPR‑associated endonuclease Cas9/Csn1 Antibody (C‑Terminus) LS‑C745371
Species: Streptococcus pyogenes
Applications: Immunofluorescence, Western blot, ELISA
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Immunofluorescence of rabbit Anti-Cas9 Antibody. Cells: HeLa cells transfected with GFP+Cas9 or GFP+CD-3. Fixation: Formaldehyde fixed, permeabilized Triton X-100. Primary: Anti-Cas9 used at 1µg/mL. Secondary: Donkey anti-Rabbit at 1µg/mL, staining seen in red. Bottom images show GFP stain overlay.
Streptococcus pyogenes CRISPR‑associated endonuclease Cas9/Csn1 Antibody (C‑Terminus, Lyophilized, Biotin) LS‑C745548
Species: Streptococcus pyogenes
Applications: Immunofluorescence, Western blot, ELISA
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Western blot analysis of extracts of wild HeLa cells and Hela transfected with cas9, using CAS9 antibody at 1:5000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 1s.
Streptococcus pyogenes CRISPR‑associated endonuclease Cas9/Csn1 Antibody LS‑C749974
Species: Streptococcus pyogenes
Applications: Western blot
TNFSF11 / RANKL / TRANCE ELISA Kit
Mouse TNFSF11 / RANKL / TRANCE (Sandwich ELISA) ELISA Kit - LS-F5674
Reactivity: Mouse
Range: 15.6-1000 pg/ml
DKK1 ELISA Kit
Mouse DKK1 (Sandwich ELISA) ELISA Kit - LS-F10347
Reactivity: Mouse
Range: 78.125-5000 pg/ml
See all Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/28/2024
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