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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1

S. pyogenes Cas9

Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Target Details

Target Name: Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1

Filtered By: None
Target
streptococcus-pyogenes-crispr-associated-endonuclease-cas9-csn1 (10)
Reactivity
All species (1)
Streptococcus pyogenes (8)
Application
WB (10)
ELISA (6)
IF (7)
IP (1)
Host
rabbit (9)
mouse (1)
Isotype
IgG (7)
IgG1,k (1)
Clonality
monoclonal mc (1)
polyclonal pc (9)
Format
Biotin Conjugated (2)
HRP Conjugated (2)
Unconjugated (6)
Epitope
aa1150-1200 (1)
Publications
No (10)
Showing 1-10 of 10 results

Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody (aa1150-1200) LS-C340585
Rabbit Polyclonal to Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1
Streptococcus pyogenes
WB
Unconjugated
100µl 1mg/ml  $325.00
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCAS-Guide vector (GE100002, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CAS9.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCAS-Guide vector (GE100002, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CAS9.

Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody LS-C745371
Rabbit Polyclonal (IgG)
Streptococcus pyogenes
ELISA IF WB
Unconjugated
100µg  $425.00
Immunofluorescence of rabbit Anti-Cas9 Antibody. Cells: HeLa cells transfected with GFP+Cas9 or GFP+CD-3. Fixation: Formaldehyde fixed, permeabilized Triton X-100. Primary: Anti-Cas9 used at 1µg/mL. Secondary: Donkey anti-Rabbit at 1µg/mL, staining seen in red. Bottom images show GFP stain overlay.
Western Blot of rabbit Anti-Cas9 Antibody. Lane 1: 293T non transfected cell lysate. Lane 2: 293T Cas9 over expressed cell lysate. Load: 15µg per lane. Primary Antibody cas9 used at 1µg/mL using MB-070 overnight at 4°C. Secondary Antibody: goat anti-rabbit HRP at 1:40,000 for 30 min at room temp. Expect: 158kDa.
Immunofluorescence of rabbit Anti-Cas9 Antibody. Cells: HeLa cells transfected with GFP+Cas9 or GFP+CD-3. Fixation: Formaldehyde fixed, permeabilized Triton X-100. Primary: Anti-Cas9 used at 1µg/mL. Secondary: Donkey anti-Rabbit at 1µg/mL, staining seen in red. Bottom images show GFP stain overlay.
Western Blot of rabbit Anti-Cas9 Antibody. Lane 1: 293T non transfected cell lysate. Lane 2: 293T Cas9 over expressed cell lysate. Load: 15µg per lane. Primary Antibody cas9 used at 1µg/mL using MB-070 overnight at 4°C. Secondary Antibody: goat anti-rabbit HRP at 1:40,000 for 30 min at room temp. Expect: 158kDa.

Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody LS-C745374
Rabbit Polyclonal (IgG)
Streptococcus pyogenes
ELISA IF WB
Unconjugated
25µl  $235.00
Immunofluorescence of rabbit Anti-Cas9 Antibody. Cells: HeLa cells transfected with GFP+Cas9 or GFP+CD-3. Fixation: Formaldehyde fixed, permeabilized Triton X-100. Primary: Anti-Cas9 used at 1µg/mL. Secondary: Donkey anti-Rabbit at 1µg/mL, staining seen in red. Bottom images show GFP stain overlay.
Western Blot of rabbit Anti-Cas9 Antibody. Lane 1: 293T non transfected cell lysate. Lane 2: 293T Cas9 over expressed cell lysate. Load: 15µg per lane. Primary Antibody cas9 used at 1µg/mL using MB-070 overnight at 4°C. Secondary Antibody: goat anti-rabbit HRP at 1:40,000 for 30 min at room temp. Expect: 158kDa.
Immunofluorescence of rabbit Anti-Cas9 Antibody. Cells: HeLa cells transfected with GFP+Cas9 or GFP+CD-3. Fixation: Formaldehyde fixed, permeabilized Triton X-100. Primary: Anti-Cas9 used at 1µg/mL. Secondary: Donkey anti-Rabbit at 1µg/mL, staining seen in red. Bottom images show GFP stain overlay.
Western Blot of rabbit Anti-Cas9 Antibody. Lane 1: 293T non transfected cell lysate. Lane 2: 293T Cas9 over expressed cell lysate. Load: 15µg per lane. Primary Antibody cas9 used at 1µg/mL using MB-070 overnight at 4°C. Secondary Antibody: goat anti-rabbit HRP at 1:40,000 for 30 min at room temp. Expect: 158kDa.

Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody LS-C658635
Mouse Monoclonal (IgG1,k)
IF IP WB
Unconjugated
50µg  $710.00

Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody (HRP) LS-C745530
Rabbit Polyclonal (IgG)
Streptococcus pyogenes
ELISA IF WB
HRP Conjugated
25µl  $235.00
Immunofluorescence of rabbit Anti-Cas9 Antibody. Cells: HeLa cells transfected with GFP+Cas9 or GFP+CD-3. Fixation: Formaldehyde fixed, permeabilized Triton X-100. Primary: Anti-Cas9 used at 1µg/mL. Secondary: Donkey anti-Rabbit at 1µg/mL, staining seen in red. Bottom images show GFP stain overlay.
Western Blot of rabbit Anti-Cas9 Antibody. Lane 1: 293T non transfected cell lysate. Lane 2: 293T Cas9 over expressed cell lysate. Load: 15µg per lane. Primary Antibody cas9 used at 1µg/mL using MB-070 overnight at 4°C. Secondary Antibody: goat anti-rabbit HRP at 1:40,000 for 30 min at room temp. Expect: 158kDa.
Immunofluorescence of rabbit Anti-Cas9 Antibody. Cells: HeLa cells transfected with GFP+Cas9 or GFP+CD-3. Fixation: Formaldehyde fixed, permeabilized Triton X-100. Primary: Anti-Cas9 used at 1µg/mL. Secondary: Donkey anti-Rabbit at 1µg/mL, staining seen in red. Bottom images show GFP stain overlay.
Western Blot of rabbit Anti-Cas9 Antibody. Lane 1: 293T non transfected cell lysate. Lane 2: 293T Cas9 over expressed cell lysate. Load: 15µg per lane. Primary Antibody cas9 used at 1µg/mL using MB-070 overnight at 4°C. Secondary Antibody: goat anti-rabbit HRP at 1:40,000 for 30 min at room temp. Expect: 158kDa.

Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody (Biotin) LS-C745547
Rabbit Polyclonal (IgG)
Streptococcus pyogenes
ELISA IF WB
Biotin Conjugated
25µl  $235.00
Immunofluorescence of rabbit Anti-Cas9 Antibody. Cells: HeLa cells transfected with GFP+Cas9 or GFP+CD-3. Fixation: Formaldehyde fixed, permeabilized Triton X-100. Primary: Anti-Cas9 used at 1µg/mL. Secondary: Donkey anti-Rabbit at 1µg/mL, staining seen in red. Bottom images show GFP stain overlay.
Western Blot of rabbit Anti-Cas9 Antibody. Lane 1: 293T non transfected cell lysate. Lane 2: 293T Cas9 over expressed cell lysate. Load: 15µg per lane. Primary Antibody cas9 used at 1µg/mL using MB-070 overnight at 4°C. Secondary Antibody: goat anti-rabbit HRP at 1:40,000 for 30 min at room temp. Expect: 158kDa.
Immunofluorescence of rabbit Anti-Cas9 Antibody. Cells: HeLa cells transfected with GFP+Cas9 or GFP+CD-3. Fixation: Formaldehyde fixed, permeabilized Triton X-100. Primary: Anti-Cas9 used at 1µg/mL. Secondary: Donkey anti-Rabbit at 1µg/mL, staining seen in red. Bottom images show GFP stain overlay.
Western Blot of rabbit Anti-Cas9 Antibody. Lane 1: 293T non transfected cell lysate. Lane 2: 293T Cas9 over expressed cell lysate. Load: 15µg per lane. Primary Antibody cas9 used at 1µg/mL using MB-070 overnight at 4°C. Secondary Antibody: goat anti-rabbit HRP at 1:40,000 for 30 min at room temp. Expect: 158kDa.

Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody (Biotin) LS-C745548
Rabbit Polyclonal (IgG)
Streptococcus pyogenes
ELISA IF WB
Biotin Conjugated
100µg  $425.00
Immunofluorescence of rabbit Anti-Cas9 Antibody. Cells: HeLa cells transfected with GFP+Cas9 or GFP+CD-3. Fixation: Formaldehyde fixed, permeabilized Triton X-100. Primary: Anti-Cas9 used at 1µg/mL. Secondary: Donkey anti-Rabbit at 1µg/mL, staining seen in red. Bottom images show GFP stain overlay.
Western Blot of rabbit Anti-Cas9 Antibody. Lane 1: 293T non transfected cell lysate. Lane 2: 293T Cas9 over expressed cell lysate. Load: 15µg per lane. Primary Antibody cas9 used at 1µg/mL using MB-070 overnight at 4°C. Secondary Antibody: goat anti-rabbit HRP at 1:40,000 for 30 min at room temp. Expect: 158kDa.
Immunofluorescence of rabbit Anti-Cas9 Antibody. Cells: HeLa cells transfected with GFP+Cas9 or GFP+CD-3. Fixation: Formaldehyde fixed, permeabilized Triton X-100. Primary: Anti-Cas9 used at 1µg/mL. Secondary: Donkey anti-Rabbit at 1µg/mL, staining seen in red. Bottom images show GFP stain overlay.
Western Blot of rabbit Anti-Cas9 Antibody. Lane 1: 293T non transfected cell lysate. Lane 2: 293T Cas9 over expressed cell lysate. Load: 15µg per lane. Primary Antibody cas9 used at 1µg/mL using MB-070 overnight at 4°C. Secondary Antibody: goat anti-rabbit HRP at 1:40,000 for 30 min at room temp. Expect: 158kDa.

Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody (HRP) LS-C745527
Rabbit Polyclonal (IgG)
Streptococcus pyogenes
ELISA IF WB
HRP Conjugated
100µg  $425.00
Immunofluorescence of rabbit Anti-Cas9 Antibody. Cells: HeLa cells transfected with GFP+Cas9 or GFP+CD-3. Fixation: Formaldehyde fixed, permeabilized Triton X-100. Primary: Anti-Cas9 used at 1µg/mL. Secondary: Donkey anti-Rabbit at 1µg/mL, staining seen in red. Bottom images show GFP stain overlay.
Western Blot of rabbit Anti-Cas9 Antibody. Lane 1: 293T non transfected cell lysate. Lane 2: 293T Cas9 over expressed cell lysate. Load: 15µg per lane. Primary Antibody cas9 used at 1µg/mL using MB-070 overnight at 4°C. Secondary Antibody: goat anti-rabbit HRP at 1:40,000 for 30 min at room temp. Expect: 158kDa.
Immunofluorescence of rabbit Anti-Cas9 Antibody. Cells: HeLa cells transfected with GFP+Cas9 or GFP+CD-3. Fixation: Formaldehyde fixed, permeabilized Triton X-100. Primary: Anti-Cas9 used at 1µg/mL. Secondary: Donkey anti-Rabbit at 1µg/mL, staining seen in red. Bottom images show GFP stain overlay.
Western Blot of rabbit Anti-Cas9 Antibody. Lane 1: 293T non transfected cell lysate. Lane 2: 293T Cas9 over expressed cell lysate. Load: 15µg per lane. Primary Antibody cas9 used at 1µg/mL using MB-070 overnight at 4°C. Secondary Antibody: goat anti-rabbit HRP at 1:40,000 for 30 min at room temp. Expect: 158kDa.

Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody LS-C742126
Rabbit Polyclonal to All species Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1
All species
WB
Unconjugated
40µl  $225.00; 100µl  $295.00
High Sensitivity: GenCrispr Cas9 antibody can detect as low as 0.5 ng GenCrispr Cas9 nuclease. GenCrispr Cas9 protein was loaded on a gel as indicated and then detected by Western blot using GenCrispr Cas9 antibody (1:3000 dilution) and Goat Anti-Rabbit IgG (H&L) [HRP],pAb (1: 10000 dilution).
High Sensitivity: GenCrispr Cas9 antibody can detect as low as 0.5 ng GenCrispr Cas9 nuclease. GenCrispr Cas9 protein was loaded on a gel as indicated and then detected by Western blot using GenCrispr Cas9 antibody (1:3000 dilution) and Goat Anti-Rabbit IgG (H&L) [HRP],pAb (1: 10000 dilution).

Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody LS-C749974
Rabbit Polyclonal (IgG) to Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1
Streptococcus pyogenes
WB
Unconjugated
50µl  $235.00
Western blot analysis of extracts of wild HeLa cells and Hela transfected with cas9, using CAS9 antibody at 1:5000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 1s.
Western blot analysis of extracts of wild HeLa cells and Hela transfected with cas9, using CAS9 antibody at 1:5000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 1s.
Showing 1-10 of 10 results

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PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).


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