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Antibodies > Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 > LS-C829085
 
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Catalog Number Size Price
LS-C829085-40 40 µg (0.5 mg/ml) $295 
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Western Blot of Hela transfected with PX458 (SaCas9(BB)-2A-GFP) or untransfected cell lysates with SaCas9 Antibody (11C12). The different concentration of cell lysates indicates the high affinity and sensitivity of the antibody. Predicted band size: 124 kDa. Predicted band size of recombinant protein: 47.5 kDa. Loading: Lane 1: 50 µg Hela transfected with SaCas9(BB)-2A-GFP cell Lysate. Lane 2: 25 µg Hela transfected with SaCas9(BB)-2A-GFP cell Lysate. Lane 3: 10 µg Hela transfected with SaCas9(BB)-2A-GFP cell Lysate. Lane 4: 50 µg Untransfected Hela cell Lysate. Lane 5: 40 ng SaCas9 recombinant protein. Primary Antibody: SaCas9 Antibody (11C12) 1 µg/ml. Secondary Antibody: Goat anti-Mouse IgG (H&L) [IRDye800] 0.125 µg/ml.
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Western Blot of Hela transfected with PX458 (SaCas9(BB)-2A-GFP) or untransfected cell lysates with two independent antibodies: SaCas9 Antibody (11C12) and SaCas9 Antibody (26H10). The correlated pattern indicates the high specificity of these two antibodies. Predicted band size: 124 kDa. Predicted band size of recombinant protein: 47.5 kDa. Loading: Lane 1: 50 µg Hela transfected with SaCas9(BB)-2A-GFP cell Lysate. Lane 2: 50 µg Untransfected Hela cell Lysate. Lane 3: 40 ng SaCas9 recombinant protein. Lane 4: 50 µg Hela transfected with SaCas9(BB)-2A-GFP cell Lysate. Lane 5: 50 µg Untransfected Hela cell Lysate. Lane 6: 40 ng SaCas9 recombinant protein. Primary Antibody: Lane 1~3: SaCas9 Antibody (11C12) 1 µg/ml. Lane 4~6: SaCas9 Antibody (26H10) 1 µg/ml. Secondary Antibody: Goat anti-Mouse IgG (H&L) [IRDye800] 0.125 µg/ml.
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Immunoprecipitation of SaCas9 from Hela cell transfected with PX458 (SaCas9(BB)-2A-GFP) by using SaCas9 Antibody (11C12). Predicted band size: 124 kDa. Loading: Lane 1: Hela cell lysate transfected with PX458 (SaCas9(BB)-2A-GFP) 50µg (Input). Lane 2: Protein A MagBeads (20µl) + Purified antibody (10µg) + Hela cell lysate transfected with PX458 (SaCas9(BB)-2A-GFP) (200µg). Lane 3: Protein A MagBeads (20µl) + Purified antibody (10µg) + Hela cell lysate (200µg). Lane 4: Protein A MagBeads (20µl) + Mouse IgG (10µg) + Hela cell lysate transfected with PX458 (SaCas9(BB)-2A-GFP) (200µg). Lane 5: Protein A MagBeads (20µl) + Mouse IgG (10µg) + Hela Cell lysate (200µg). Lane 6: Hela Cell lysate (50µg). Primary Antibody: SaCas9 Antibody (11C12) 1 µg/ml. Secondary antibody: Goat anti-Mouse IgG (H&L) [IRDye800] 0.125 µg/ml.
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Western Blot analysis of Hela transfected with various plasmids with SaCas9 Antibody (11C12). The different Hela transfected with various plasmids indicate the minimum cross reaction of the antibody. Predicted band size: 124 kDa. Loading: Lane 1: 50µg Hela cell lysate transfected with StCas9(BB)-2A-GFP (G3ECR1, Streptococcus thermophiles). Lane 2: 50µg Hela cell lysate transfected with pSpCas9(BB)-2A-GFP (PX458, Q99ZW2, Streptococcus pyogenes serotype M1). Lane 3: 50µg Hela cell lysate transfected with SaCas9(BB)-2A-GFP (J7RUA5, Staphylococcus aureus). Lane 4: 50µg Hela cell lysate transfected with AsCpf1(BB)-2A-GFP (U2UMQ6, Acidaminococcus sp. (strain BV3L6)). Lane 5: 50µg Hela cell lysate transfected with FnCpf1(BB)-2A-GFP (Francisella tularensis subsp. novicida (strain U112)). Lane 6: 50µg Hela cell lysate transfected with LbCpf1(BB)-2A-GFP (Lachnospiraceae bacterium ND2006). Lane 7: 50µg Hela cell lysate (Non-transfected). Primary Antibody: SaCas9 Antibody (11C12) 1 µg/ml. Secondary antibody: Goat anti-Mouse IgG (H&L) [IRDye800] 0.125 µg/ml.
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Standard curve of SaCas9 Sandwich ELISA. The SaCas9 Sandwich ELISA assay is developed by using SaCas9 Antibody (11C12) and SaCas9 Antibody (26H10) as capture and detection antibody, respectively. These two antibodies recognize different epitopes. In this ELISA assay, SaCas9 Antibody (26H10) was labeled with Biotin. The sensitivity is < 1 ng/ml and the detection range is 0-30 ng/ml.
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - ELISA binding of SaCas9 Antibody (11C12) with recombinant Staphylococcus aureus CRISPR/Cas9 protein. Coating antigen: SaCas9, 1 ug/ml. SaCas9 antibody dilution start from 1000 ng/ml,EC50= 3.75 ng/ml.
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Western Blot of Hela transfected with PX458 (SaCas9(BB)-2A-GFP) or untransfected cell lysates with SaCas9 Antibody (11C12). The different concentration of cell lysates indicates the high affinity and sensitivity of the antibody. Predicted band size: 124 kDa. Predicted band size of recombinant protein: 47.5 kDa. Loading: Lane 1: 50 µg Hela transfected with SaCas9(BB)-2A-GFP cell Lysate. Lane 2: 25 µg Hela transfected with SaCas9(BB)-2A-GFP cell Lysate. Lane 3: 10 µg Hela transfected with SaCas9(BB)-2A-GFP cell Lysate. Lane 4: 50 µg Untransfected Hela cell Lysate. Lane 5: 40 ng SaCas9 recombinant protein. Primary Antibody: SaCas9 Antibody (11C12) 1 µg/ml. Secondary Antibody: Goat anti-Mouse IgG (H&L) [IRDye800] 0.125 µg/ml.
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Western Blot of Hela transfected with PX458 (SaCas9(BB)-2A-GFP) or untransfected cell lysates with two independent antibodies: SaCas9 Antibody (11C12) and SaCas9 Antibody (26H10). The correlated pattern indicates the high specificity of these two antibodies. Predicted band size: 124 kDa. Predicted band size of recombinant protein: 47.5 kDa. Loading: Lane 1: 50 µg Hela transfected with SaCas9(BB)-2A-GFP cell Lysate. Lane 2: 50 µg Untransfected Hela cell Lysate. Lane 3: 40 ng SaCas9 recombinant protein. Lane 4: 50 µg Hela transfected with SaCas9(BB)-2A-GFP cell Lysate. Lane 5: 50 µg Untransfected Hela cell Lysate. Lane 6: 40 ng SaCas9 recombinant protein. Primary Antibody: Lane 1~3: SaCas9 Antibody (11C12) 1 µg/ml. Lane 4~6: SaCas9 Antibody (26H10) 1 µg/ml. Secondary Antibody: Goat anti-Mouse IgG (H&L) [IRDye800] 0.125 µg/ml.
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Immunoprecipitation of SaCas9 from Hela cell transfected with PX458 (SaCas9(BB)-2A-GFP) by using SaCas9 Antibody (11C12). Predicted band size: 124 kDa. Loading: Lane 1: Hela cell lysate transfected with PX458 (SaCas9(BB)-2A-GFP) 50µg (Input). Lane 2: Protein A MagBeads (20µl) + Purified antibody (10µg) + Hela cell lysate transfected with PX458 (SaCas9(BB)-2A-GFP) (200µg). Lane 3: Protein A MagBeads (20µl) + Purified antibody (10µg) + Hela cell lysate (200µg). Lane 4: Protein A MagBeads (20µl) + Mouse IgG (10µg) + Hela cell lysate transfected with PX458 (SaCas9(BB)-2A-GFP) (200µg). Lane 5: Protein A MagBeads (20µl) + Mouse IgG (10µg) + Hela Cell lysate (200µg). Lane 6: Hela Cell lysate (50µg). Primary Antibody: SaCas9 Antibody (11C12) 1 µg/ml. Secondary antibody: Goat anti-Mouse IgG (H&L) [IRDye800] 0.125 µg/ml.
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Western Blot analysis of Hela transfected with various plasmids with SaCas9 Antibody (11C12). The different Hela transfected with various plasmids indicate the minimum cross reaction of the antibody. Predicted band size: 124 kDa. Loading: Lane 1: 50µg Hela cell lysate transfected with StCas9(BB)-2A-GFP (G3ECR1, Streptococcus thermophiles). Lane 2: 50µg Hela cell lysate transfected with pSpCas9(BB)-2A-GFP (PX458, Q99ZW2, Streptococcus pyogenes serotype M1). Lane 3: 50µg Hela cell lysate transfected with SaCas9(BB)-2A-GFP (J7RUA5, Staphylococcus aureus). Lane 4: 50µg Hela cell lysate transfected with AsCpf1(BB)-2A-GFP (U2UMQ6, Acidaminococcus sp. (strain BV3L6)). Lane 5: 50µg Hela cell lysate transfected with FnCpf1(BB)-2A-GFP (Francisella tularensis subsp. novicida (strain U112)). Lane 6: 50µg Hela cell lysate transfected with LbCpf1(BB)-2A-GFP (Lachnospiraceae bacterium ND2006). Lane 7: 50µg Hela cell lysate (Non-transfected). Primary Antibody: SaCas9 Antibody (11C12) 1 µg/ml. Secondary antibody: Goat anti-Mouse IgG (H&L) [IRDye800] 0.125 µg/ml.
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Standard curve of SaCas9 Sandwich ELISA. The SaCas9 Sandwich ELISA assay is developed by using SaCas9 Antibody (11C12) and SaCas9 Antibody (26H10) as capture and detection antibody, respectively. These two antibodies recognize different epitopes. In this ELISA assay, SaCas9 Antibody (26H10) was labeled with Biotin. The sensitivity is < 1 ng/ml and the detection range is 0-30 ng/ml.
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - ELISA binding of SaCas9 Antibody (11C12) with recombinant Staphylococcus aureus CRISPR/Cas9 protein. Coating antigen: SaCas9, 1 ug/ml. SaCas9 antibody dilution start from 1000 ng/ml,EC50= 3.75 ng/ml.
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Monoclonal Mouse anti‑Staphylococcus aureus Streptococcus pyogenes CRISPR‑associated endonuclease Cas9/Csn1 Antibody (clone 11C12, IF, WB) LS‑C829085

Monoclonal Mouse anti‑Staphylococcus aureus Streptococcus pyogenes CRISPR‑associated endonuclease Cas9/Csn1 Antibody (clone 11C12, IF, WB) LS‑C829085

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Antibody:
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Mouse anti-Staphylococcus aureus Monoclonal (11C12) Antibody
Application:
IF, WB, ELISA
Reactivity:
Staphylococcus aureus
Format:
Unconjugated, Unmodified
Price
Catalog Number
$295
LS-C829085-40
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Mouse anti-Staphylococcus aureus Monoclonal (11C12) Antibody
Application:
IF, WB, ELISA
Reactivity:
Staphylococcus aureus
Format:
Unconjugated, Unmodified

Specifications

Description
Csn1 antibody LS-C829085 is an unconjugated mouse monoclonal antibody to staphylococcus aureus Csn1 (Streptococcus pyogenes CRISPR-associated endonuclease Cas9). Validated for ELISA, IF and WB.
Target
Staphylococcus aureus Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1
Host
Mouse
Reactivity
Staphylococcus aureus (tested or 100% immunogen sequence identity)
Clonality
IgG2b,k Monoclonal
Clone
11C12
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Recombinant Staphylococcus aureus CRISPR/Cas9
Specificity
The product is specific for Staphylococcus aureus CRISPR/Cas9. This antibody binds with recombinant Staphylococcus aureus CRISPR/Cas9 protein in ELISA and Endogenous overexpressed Staphylococcus aureus CRISPR/Cas9 in immunofluorescence, western blot, immunoprecipitation.
Applications
  • Immunofluorescence (0.2 - 1 µg/ml)
  • Western blot (1 µg/ml)
  • ELISA (0.01 - 1 µg/ml)
Usage
Applications should be user optimized.
Presentation
Lyophilized from PBS, pH 7.4, 0.02% Sodium Azide
Reconstitution
Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Storage
Store lyophilized at -20°C for up to 1 year. Store reconstituted at 2°C to 8°C for up to 3 weeks. Aliquot and store at -20°C or below for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Featured Products

Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCAS-Guide vector (GE100002, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CAS9.
Streptococcus pyogenes CRISPR‑associated endonuclease Cas9/Csn1 Antibody (aa1150‑1200) LS‑C340585
Species: Streptococcus pyogenes
Applications: Western blot
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Immunofluorescence of rabbit Anti-Cas9 Antibody. Cells: HeLa cells transfected with GFP+Cas9 or GFP+CD-3. Fixation: Formaldehyde fixed, permeabilized Triton X-100. Primary: Anti-Cas9 used at 1µg/mL. Secondary: Donkey anti-Rabbit at 1µg/mL, staining seen in red. Bottom images show GFP stain overlay.
Streptococcus pyogenes CRISPR‑associated endonuclease Cas9/Csn1 Antibody (C‑Terminus) LS‑C745371
Species: Streptococcus pyogenes
Applications: Immunofluorescence, Western blot, ELISA
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Immunofluorescence of rabbit Anti-Cas9 Antibody. Cells: HeLa cells transfected with GFP+Cas9 or GFP+CD-3. Fixation: Formaldehyde fixed, permeabilized Triton X-100. Primary: Anti-Cas9 used at 1µg/mL. Secondary: Donkey anti-Rabbit at 1µg/mL, staining seen in red. Bottom images show GFP stain overlay.
Streptococcus pyogenes CRISPR‑associated endonuclease Cas9/Csn1 Antibody (C‑Terminus, Lyophilized, Biotin) LS‑C745548
Species: Streptococcus pyogenes
Applications: Immunofluorescence, Western blot, ELISA
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Western blot analysis of extracts of wild HeLa cells and Hela transfected with cas9, using CAS9 antibody at 1:5000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 1s.
Streptococcus pyogenes CRISPR‑associated endonuclease Cas9/Csn1 Antibody LS‑C749974
Species: Streptococcus pyogenes
Applications: Western blot
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Species: Human
Applications: IHC, IHC - Paraffin, IHC - Frozen
c-Met ELISA Kit
Mouse/Human/Rat Phospho-c-Met ELISA Kit - LS-F340
Reactivity: Human, Mouse, Rat
Range:
See all Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibodies

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Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/28/2024
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