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Catalog Number Size Price
LS-C745374-25 25 µl (1 mg/ml) $275 
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Immunofluorescence of rabbit Anti-Cas9 Antibody. Cells: HeLa cells transfected with GFP+Cas9 or GFP+CD-3. Fixation: Formaldehyde fixed, permeabilized Triton X-100. Primary: Anti-Cas9 used at 1µg/mL. Secondary: Donkey anti-Rabbit at 1µg/mL, staining seen in red. Bottom images show GFP stain overlay.
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Western Blot results of rabbit Anti-Cas 9 Antibody. Lane 1: Opal Prestained Molecular Weight Ladder Lane 2: HEK/293 Cas9 Over Expressing WCL. Lane 3: HEK/293 non Transfected WCL. Load: 10ul. Primary Antibody: Rabbit Anti-Cas 9 Antibody at 1µg/mL overnight at 4°C. Secondary Antibody: Goat anti-Rabbit HRP at 1:70,000 for 30min at RT. Blocking: BlockOut for 30 min at RT.
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Western Blot of rabbit Anti-Cas9 Antibody. Lane 1: 293T non transfected cell lysate. Lane 2: 293T Cas9 over expressed cell lysate. Load: 15µg per lane. Primary Antibody cas9 used at 1µg/mL using MB-070 overnight at 4°C. Secondary Antibody: goat anti-rabbit HRP at 1:40,000 for 30 min at room temp. Expect: 158kDa.
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Immunofluorescence of rabbit Anti-Cas9 Antibody. Cells: HeLa cells transfected with GFP+Cas9 or GFP+CD-3. Fixation: Formaldehyde fixed, permeabilized Triton X-100. Primary: Anti-Cas9 used at 1µg/mL. Secondary: Donkey anti-Rabbit at 1µg/mL, staining seen in red. Bottom images show GFP stain overlay.
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Western Blot results of rabbit Anti-Cas 9 Antibody. Lane 1: Opal Prestained Molecular Weight Ladder Lane 2: HEK/293 Cas9 Over Expressing WCL. Lane 3: HEK/293 non Transfected WCL. Load: 10ul. Primary Antibody: Rabbit Anti-Cas 9 Antibody at 1µg/mL overnight at 4°C. Secondary Antibody: Goat anti-Rabbit HRP at 1:70,000 for 30min at RT. Blocking: BlockOut for 30 min at RT.
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Western Blot of rabbit Anti-Cas9 Antibody. Lane 1: 293T non transfected cell lysate. Lane 2: 293T Cas9 over expressed cell lysate. Load: 15µg per lane. Primary Antibody cas9 used at 1µg/mL using MB-070 overnight at 4°C. Secondary Antibody: goat anti-rabbit HRP at 1:40,000 for 30 min at room temp. Expect: 158kDa.
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Polyclonal Rabbit anti‑Streptococcus pyogenes Streptococcus pyogenes CRISPR‑associated endonuclease Cas9/Csn1 Antibody (C‑Terminus, IF, WB) LS‑C745374

Polyclonal Rabbit anti‑Streptococcus pyogenes Streptococcus pyogenes CRISPR‑associated endonuclease Cas9/Csn1 Antibody (C‑Terminus, IF, WB) LS‑C745374

Antibody:
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Rabbit anti-Streptococcus pyogenes Polyclonal (C-Terminus) Antibody
Application:
IF, WB, ELISA
Reactivity:
Streptococcus pyogenes
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C745374-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Rabbit anti-Streptococcus pyogenes Polyclonal (C-Terminus) Antibody
Application:
IF, WB, ELISA
Reactivity:
Streptococcus pyogenes
Format:
Unconjugated, Unmodified

Specifications

Description
Csn1 antibody LS-C745374 is an unconjugated rabbit polyclonal antibody to streptococcus pyogenes Csn1 (Streptococcus pyogenes CRISPR-associated endonuclease Cas9) (C-Terminus). Validated for ELISA, IF and WB.
Target
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1
Host
Rabbit
Reactivity
Streptococcus pyogenes (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Cas-9 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal of Streptococcus pyogenes Cas-9 protein.
Epitope
C-Terminus
Applications
  • Immunofluorescence (1 µg/ml)
  • Western blot (1 µg/ml)
  • ELISA (1:25000 - 1:30000)
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/17/2021