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Antibodies > Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 > LS-C772951
 
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Catalog Number Size Price
LS-C772951-100 100 µl $469 
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Immunofluorescence of HEK293 cells transfected with a fusion construct of GFP with the C-terminal region of S. aureas Cas9 protein (green) that is labeled with Anti-CRISPR Cas9 (1:1000, red)and the DNA stained blue with DAPI. The yellow label is the overlapping of the Anti-Cas9 antibody and the Cas9-GFP construct.m Download the protocol used for this image:
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Immunofluorescence of HEK293 cells transfected with S. aureas Cas9 and labeled with Anti-CRISPR Cas9 (1:500, red).
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Western blot of GFP-Cas9 transfected HEK293 cell lysate (+) showing specific immunolabeling of the ~53 kDa GFP-Cas9 fusion protein. Labeling is absent in the untransfected lysate (-). Click here to view our Western blotting and lysate preparation protocols.
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Immunofluorescence of HEK293 cells transfected with a fusion construct of GFP with the C-terminal region of S. aureas Cas9 protein (green) that is labeled with Anti-CRISPR Cas9 (1:1000, red)and the DNA stained blue with DAPI. The yellow label is the overlapping of the Anti-Cas9 antibody and the Cas9-GFP construct.m Download the protocol used for this image:
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Immunofluorescence of HEK293 cells transfected with S. aureas Cas9 and labeled with Anti-CRISPR Cas9 (1:500, red).
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Western blot of GFP-Cas9 transfected HEK293 cell lysate (+) showing specific immunolabeling of the ~53 kDa GFP-Cas9 fusion protein. Labeling is absent in the untransfected lysate (-). Click here to view our Western blotting and lysate preparation protocols.
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Monoclonal Mouse anti‑Staphylococcus aureus Streptococcus pyogenes CRISPR‑associated endonuclease Cas9/Csn1 Antibody (clone 6F7, C‑term, IHC, IF, WB) LS‑C772951

Monoclonal Mouse anti‑Staphylococcus aureus Streptococcus pyogenes CRISPR‑associated endonuclease Cas9/Csn1 Antibody (clone 6F7, C‑term, IHC, IF, WB) LS‑C772951

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Antibody:
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Mouse anti-Staphylococcus aureus Monoclonal (C-term) (6F7) Antibody
Application:
IHC, IF, WB
Reactivity:
Staphylococcus aureus
Format:
Unconjugated, Unmodified
Price
Catalog Number
$469
LS-C772951-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Mouse anti-Staphylococcus aureus Monoclonal (C-term) (6F7) Antibody
Application:
IHC, IF, WB
Reactivity:
Staphylococcus aureus
Format:
Unconjugated, Unmodified

Specifications

Description
Csn1 antibody LS-C772951 is an unconjugated mouse monoclonal antibody to staphylococcus aureus Csn1 (Streptococcus pyogenes CRISPR-associated endonuclease Cas9) (C-term). Validated for IF, IHC and WB.
Target
Staphylococcus aureus Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1
Host
Mouse
Reactivity
Staphylococcus aureus (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
6F7
Conjugations
Unconjugated
Purification
Protein G purified from tissue culture supernatant
Modifications
Unmodified
Immunogen
Recombinant protein from C-Terminus of Cas9 from Staphylococcus aureus.
Epitope
C-term
Applications
  • IHC (1:1000 - 1:5000)
  • Immunofluorescence (1:500 - 1:5000)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Western blot reference weight: 53 kDa
Presentation
PBS, 5 mM Sodium Azide, 50% Glycerol
Storage
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Featured Products

Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCAS-Guide vector (GE100002, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CAS9.
Streptococcus pyogenes CRISPR‑associated endonuclease Cas9/Csn1 Antibody (aa1150‑1200) LS‑C340585
Species: Streptococcus pyogenes
Applications: Western blot
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Immunofluorescence of rabbit Anti-Cas9 Antibody. Cells: HeLa cells transfected with GFP+Cas9 or GFP+CD-3. Fixation: Formaldehyde fixed, permeabilized Triton X-100. Primary: Anti-Cas9 used at 1µg/mL. Secondary: Donkey anti-Rabbit at 1µg/mL, staining seen in red. Bottom images show GFP stain overlay.
Streptococcus pyogenes CRISPR‑associated endonuclease Cas9/Csn1 Antibody (C‑Terminus) LS‑C745371
Species: Streptococcus pyogenes
Applications: Immunofluorescence, Western blot, ELISA
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Immunofluorescence of rabbit Anti-Cas9 Antibody. Cells: HeLa cells transfected with GFP+Cas9 or GFP+CD-3. Fixation: Formaldehyde fixed, permeabilized Triton X-100. Primary: Anti-Cas9 used at 1µg/mL. Secondary: Donkey anti-Rabbit at 1µg/mL, staining seen in red. Bottom images show GFP stain overlay.
Streptococcus pyogenes CRISPR‑associated endonuclease Cas9/Csn1 Antibody (C‑Terminus, Lyophilized, Biotin) LS‑C745548
Species: Streptococcus pyogenes
Applications: Immunofluorescence, Western blot, ELISA
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Western blot analysis of extracts of wild HeLa cells and Hela transfected with cas9, using CAS9 antibody at 1:5000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 1s.
Streptococcus pyogenes CRISPR‑associated endonuclease Cas9/Csn1 Antibody LS‑C749974
Species: Streptococcus pyogenes
Applications: Western blot
SIRT6 / Sirtuin 6 ELISA Kit
Human SIRT6 / Sirtuin 6 (Sandwich ELISA) ELISA Kit - LS-F52611
Reactivity: Human
Range: 3.12-200 pg/ml
TUBA1B / Tubulin Alpha 1B ELISA Kit
Human TUBA1B / Tubulin Alpha 1B (Sandwich ELISA) ELISA Kit - LS-F32018
Reactivity: Human
Range: 0.156-10 ng/ml
See all Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/28/2024
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