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Catalog Number Size Price
LS-C796676-100 100 µg $539 
SQSTM1 Antibody - Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-SQSTM1mouse monoclonal antibody. (Clone UMAB12, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
SQSTM1 Antibody - Immunohistochemical staining of paraffin-embedded human brain using anti-SQSTM1 clone UMAB12 mouse monoclonal antibody  at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH 8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Strong cytoplasmic staining is seen in the nueral cells.
SQSTM1 Antibody - Immunohistochemical staining of paraffin-embedded human colon using anti-SQSTM1 clone UMAB12 mouse monoclonal antibody  at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH 8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Strong cytoplasmic staining is seen in the colon epithelial cells.
SQSTM1 Antibody - Immunohistochemical staining of paraffin-embedded liver tissue using anti-SQSTM1mouse monoclonal antibody. (Clone UMAB12, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
SQSTM1 Antibody - Immunohistochemical staining of paraffin-embedded colon tissue using anti-SQSTM1mouse monoclonal antibody. (Clone UMAB12, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
SQSTM1 Antibody - Immunohistochemical staining of paraffin-embedded prostate tissue using anti-SQSTM1mouse monoclonal antibody. (Clone UMAB12, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
SQSTM1 Antibody - Immunohistochemical staining of paraffin-embedded human pancreatic cancer using anti-SQSTM1 clone UMAB12 mouse monoclonal antibody  at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH 8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Reduced cytoplasmic staining is seen in the tumor cells.
SQSTM1 Antibody - Immunohistochemical staining of paraffin-embedded human kidney using anti-SQSTM1 clone UMAB12 mouse monoclonal antibody  at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH 8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Strong cytoplasmic staining is seen in the tubule epithelial cells.
SQSTM1 Antibody - Immunohistochemical staining of paraffin-embedded human colon cancer using anti-SQSTM1 clone UMAB12 mouse monoclonal antibody  at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH 8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Strong cytoplasmic staining is seen in the tumor cells.
SQSTM1 Antibody - Immunohistochemical staining of paraffin-embedded human glioma using anti-SQSTM1 clone UMAB12 mouse monoclonal antibody  at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH 8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Strong cytoplasmic staining is seen in the tumor cells.
SQSTM1 Antibody - Immunohistochemical staining of paraffin-embedded human breast cancer using anti-SQSTM1 clone UMAB12 mouse monoclonal antibody  at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH 8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Strong cytoplasmic staining is seen in the tumor cells.
SQSTM1 Antibody - Immunohistochemical staining of paraffin-embedded human ovarian cancer using anti-SQSTM1 clone UMAB12 mouse monoclonal antibody  at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH 8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Strong cytoplasmic staining is seen in the tumor cells.
SQSTM1 Antibody - Immunohistochemical staining of paraffin-embedded human liver cancer using anti-SQSTM1 clone UMAB12 mouse monoclonal antibody  at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH 8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Strong cytoplasmic staining is seen in the tumor cells.
SQSTM1 Antibody - Immunofluorescent staining of A549 cells using SQSTM1 mouse monoclonal antibody  green). Actin filaments were labeled with TRITC-phalloidin. (red), and nuclear with DAPI. (blue). The three-color overlay image is located at the bottom-right corner.
SQSTM1 Antibody - Immunofluorescent staining of HeLa cells using SQSTM1 mouse monoclonal antibody  green). Actin filaments were labeled with TRITC-phalloidin. (red), and nuclear with DAPI. (blue). The three-color overlay image is located at the bottom-right corner.
SQSTM1 Antibody - Western blot analysis of extracts. (35ug) from 9 different cell lines by using anti-SQSTM1 monoclonal antibody. (Clone UMAB12) at 1:500.
SQSTM1 Antibody - Equivalent amounts of cell lysates  and SQSTM1-Knockout 293T cells  were separated by SDS-PAGE and immunoblotted with anti-SQSTM1 monoclonal antibody(1:500). Then the blotted membrane was stripped and reprobed with anti-b-actin antibody  as a loading control.
SQSTM1 Antibody - Western blot of human tissue lysates. (15ug) from 10 different tissues. (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid 9: Colon, 10: Spleen). Diluation: 1:500.
SQSTM1 Antibody - Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-SQSTM1mouse monoclonal antibody. (Clone UMAB12, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
SQSTM1 Antibody - Immunohistochemical staining of paraffin-embedded human brain using anti-SQSTM1 clone UMAB12 mouse monoclonal antibody  at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH 8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Strong cytoplasmic staining is seen in the nueral cells.
SQSTM1 Antibody - Immunohistochemical staining of paraffin-embedded human colon using anti-SQSTM1 clone UMAB12 mouse monoclonal antibody  at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH 8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Strong cytoplasmic staining is seen in the colon epithelial cells.
SQSTM1 Antibody - Immunohistochemical staining of paraffin-embedded liver tissue using anti-SQSTM1mouse monoclonal antibody. (Clone UMAB12, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
SQSTM1 Antibody - Immunohistochemical staining of paraffin-embedded colon tissue using anti-SQSTM1mouse monoclonal antibody. (Clone UMAB12, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
SQSTM1 Antibody - Immunohistochemical staining of paraffin-embedded prostate tissue using anti-SQSTM1mouse monoclonal antibody. (Clone UMAB12, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
SQSTM1 Antibody - Immunohistochemical staining of paraffin-embedded human pancreatic cancer using anti-SQSTM1 clone UMAB12 mouse monoclonal antibody  at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH 8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Reduced cytoplasmic staining is seen in the tumor cells.
SQSTM1 Antibody - Immunohistochemical staining of paraffin-embedded human kidney using anti-SQSTM1 clone UMAB12 mouse monoclonal antibody  at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH 8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Strong cytoplasmic staining is seen in the tubule epithelial cells.
SQSTM1 Antibody - Immunohistochemical staining of paraffin-embedded human colon cancer using anti-SQSTM1 clone UMAB12 mouse monoclonal antibody  at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH 8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Strong cytoplasmic staining is seen in the tumor cells.
SQSTM1 Antibody - Immunohistochemical staining of paraffin-embedded human glioma using anti-SQSTM1 clone UMAB12 mouse monoclonal antibody  at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH 8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Strong cytoplasmic staining is seen in the tumor cells.
SQSTM1 Antibody - Immunohistochemical staining of paraffin-embedded human breast cancer using anti-SQSTM1 clone UMAB12 mouse monoclonal antibody  at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH 8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Strong cytoplasmic staining is seen in the tumor cells.
SQSTM1 Antibody - Immunohistochemical staining of paraffin-embedded human ovarian cancer using anti-SQSTM1 clone UMAB12 mouse monoclonal antibody  at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH 8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Strong cytoplasmic staining is seen in the tumor cells.
SQSTM1 Antibody - Immunohistochemical staining of paraffin-embedded human liver cancer using anti-SQSTM1 clone UMAB12 mouse monoclonal antibody  at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH 8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Strong cytoplasmic staining is seen in the tumor cells.
SQSTM1 Antibody - Immunofluorescent staining of A549 cells using SQSTM1 mouse monoclonal antibody  green). Actin filaments were labeled with TRITC-phalloidin. (red), and nuclear with DAPI. (blue). The three-color overlay image is located at the bottom-right corner.
SQSTM1 Antibody - Immunofluorescent staining of HeLa cells using SQSTM1 mouse monoclonal antibody  green). Actin filaments were labeled with TRITC-phalloidin. (red), and nuclear with DAPI. (blue). The three-color overlay image is located at the bottom-right corner.
SQSTM1 Antibody - Western blot analysis of extracts. (35ug) from 9 different cell lines by using anti-SQSTM1 monoclonal antibody. (Clone UMAB12) at 1:500.
SQSTM1 Antibody - Equivalent amounts of cell lysates  and SQSTM1-Knockout 293T cells  were separated by SDS-PAGE and immunoblotted with anti-SQSTM1 monoclonal antibody(1:500). Then the blotted membrane was stripped and reprobed with anti-b-actin antibody  as a loading control.
SQSTM1 Antibody - Western blot of human tissue lysates. (15ug) from 10 different tissues. (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid 9: Colon, 10: Spleen). Diluation: 1:500.
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Monoclonal Mouse anti‑Human SQSTM1 Antibody (clone UMAB12, Carrier‑free, IHC, IF, WB) LS‑C796676

Monoclonal Mouse anti‑Human SQSTM1 Antibody (clone UMAB12, Carrier‑free, IHC, IF, WB) LS‑C796676

Antibody:
SQSTM1 Mouse anti-Human Monoclonal (Carrier-free) (UMAB12) Antibody
Application:
IHC, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Carrier-free
Other formats:
Price
Catalog Number
$539
LS-C796676-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
SQSTM1 Mouse anti-Human Monoclonal (Carrier-free) (UMAB12) Antibody
Application:
IHC, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Carrier-free
Other formats:

Specifications

Description
SQSTM1 antibody LS-C796676 is an unconjugated mouse monoclonal antibody to human SQSTM1. Validated for Flow, IF, IHC and WB.
Target
Human SQSTM1
Synonyms
SQSTM1 | A170 | ORCA | OSIL | Paget disease of bone 3 | p60 | p62 | PDB3 | Sequestosome-1 | Ubiquitin-binding protein p62 | Oxidative stress induced like | p62B | ZIP3 | Sequestosome 1 | EBI3-associated protein p60 | EBIAP
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2b Monoclonal
Clone
UMAB12
Conjugations
Unconjugated
Purification
Purified from ascites.
Modifications
Carrier-free. Also available Unmodified.
Immunogen
Full length human recombinant protein of human SQSTM1(NP_003891) produced in HEK293T cell.
Specificity
The specificity of this antibody has been validated by testing against a high-density Protein Microarray containing more than 17,000 recombinant proteins.
Applications
  • IHC (1:150)
  • Immunofluorescence (1:100)
  • Western blot (1:500 - 1:2000)
  • Flow Cytometry
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.3, 8% Trehalose
Reconstitution
Reconstitute with PBS pH 7.3. To use this carrier-free antibody for conjugation experiment, we strongly recommend you to perform another round of desalting process.
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SQSTM1
Q13501 NM_003900 NP_003891.1

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Requested From: United States
Date Requested: 4/28/2024