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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SPRING / TRIM9 Antibody (clone 2A1) LS‑C175108
TRIM9 antibody LS-C175108 is an unconjugated mouse monoclonal antibody to human TRIM9 (SPRING). Validated for Flow and WB.
Catalog
Size
Price
LS-C175108-100
100 µl (1 mg/ml)
$335

Popular SPRING / TRIM9 Products

Anti-TRIM9 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
A: Marker, B: HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: Western blot
Anti-TRIM9 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TRIM9.
Species: Human
Applications: Immunofluorescence, Western blot, Flow Cytometry
Anti-TRIM9 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TRIM9.
Species: Human
Applications: Immunofluorescence, Western blot, Flow Cytometry
Anti-TRIM9 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TRIM9.
Species: Human
Applications: Immunofluorescence, Western blot, Flow Cytometry

Product Description

TRIM9 antibody LS-C175108 is an unconjugated mouse monoclonal antibody to human TRIM9 (SPRING). Validated for Flow and WB.

Specifications

Target
Human SPRING / TRIM9
Synonyms
TRIM9, KIAA0282, RING finger protein 91, SPRING, Tripartite motif containing 9, RNF91, Tripartite motif-containing 9
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [2A1]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • Western blot (1:2000)
  • Flow Cytometry (1:100)
Immunogen
SPRING / TRIM9 antibody was raised against human recombinant protein fragment corresponding to amino acids 284-669 of human TRIM9 (NP_443210) produced in E. coli.
Specificity
Human SPRING / TRIM9
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About SPRING / TRIM9
E3 ubiquitin-protein ligase which ubiquitinates itself in cooperation with an E2 enzyme UBE2D2/UBC4 and serves as a targeting signal for proteasomal degradation. May play a role in regulation of neuronal functions and may also participate in the formation or breakdown of abnormal inclusions in neurodegenerative disorders. May act as a regulator of synaptic vesicle exocytosis by controlling the availability of SNAP25 for the SNARE complex formation. Q9C026 NM_015163 NP_055978.4


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Images

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM9.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM9.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-TRIM9 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-TRIM9 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

Flow cytometry of HeLa cells, using anti-TRIM9 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of HeLa cells, using anti-TRIM9 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TRIM9 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TRIM9 antibody, and then analyzed by flow cytometry.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM9.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM9.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-TRIM9 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-TRIM9 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

Flow cytometry of HeLa cells, using anti-TRIM9 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of HeLa cells, using anti-TRIM9 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TRIM9 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TRIM9 antibody, and then analyzed by flow cytometry.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM9.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM9.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-TRIM9 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-TRIM9 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

Flow cytometry of HeLa cells, using anti-TRIM9 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of HeLa cells, using anti-TRIM9 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TRIM9 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TRIM9 antibody, and then analyzed by flow cytometry.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM9.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM9.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-TRIM9 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-TRIM9 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

Flow cytometry of HeLa cells, using anti-TRIM9 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of HeLa cells, using anti-TRIM9 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TRIM9 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TRIM9 antibody, and then analyzed by flow cytometry.

Requested From: United States
Date Requested: 11/16/2018

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