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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SPECC1 / CYTSB Antibody (aa1-100) IHC-plus™ LS-B1735

Note: This antibody replaces LS-C2821
CYTSB antibody LS-B1735 is an unconjugated rabbit polyclonal antibody to human CYTSB (SPECC1). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B1735-50
50 µl
$425
Description
CYTSB antibody LS-B1735 is an unconjugated rabbit polyclonal antibody to human CYTSB (SPECC1). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human SPECC1 / CYTSB
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, DY650, DY488, DY550.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (20 µg/ml)
  • Western blot (1:500 - 1:2000)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
SPECC1 / CYTSB antibody was raised against an internal synthetic peptide made to the human NSP5a3a protein sequence (between residues 1-100) Percent identity by BLAST analysis: Mouse, Rat (83%).
Epitope
aa1-100
Specificity
NSP5a3a.
Usage
Immunohistochemistry: LS-B1735 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1735 was determined to be 20 ug/ml.
Presentation
Tris-citrate/phosphate buffer, pH 7-8.
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About SPECC1 / CYTSB

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Images

Immunohistochemistry

Anti-SPECC1 / CYTSB antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-SPECC1 / CYTSB antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-SPECC1 / CYTSB antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-SPECC1 / CYTSB antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-SPECC1 / CYTSB antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-SPECC1 / CYTSB antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-SPECC1 / CYTSB antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-SPECC1 / CYTSB antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 3/18/2019
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