CYTSB antibody LS-B1735 is an unconjugated rabbit polyclonal antibody to human CYTSB (SPECC1). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Human SPECC1 / CYTSB
(tested or 100% immunogen sequence identity)
Also available conjugated with Biotin
- IHC - Paraffin (20 µg/ml)
- Western blot (1:500 - 1:2000)
- (applications tested for the base form of this product only)
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SPECC1 / CYTSB antibody was raised against an internal synthetic peptide made to the human NSP5a3a protein sequence (between residues 1-100) Percent identity by BLAST analysis: Mouse, Rat (83%).
Immunohistochemistry: LS-B1735 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1735 was determined to be 20 ug/ml.
Tris-citrate/phosphate buffer, pH 7-8.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.