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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SPECC1 / CYTSB Antibody (aa705‑733) LS‑C161441
CYTSB antibody LS-C161441 is an unconjugated rabbit polyclonal antibody to CYTSB (SPECC1) from human and mouse. Validated for Flow, IF, IHC and WB.
Catalog
Size
Price
LS-C161441-400
400 µl
$305
Description
CYTSB antibody LS-C161441 is an unconjugated rabbit polyclonal antibody to CYTSB (SPECC1) from human and mouse. Validated for Flow, IF, IHC and WB.
Target
Human SPECC1 / CYTSB
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Predicted
Chicken, Zebrafish (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:10 - 1:50)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E1445 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C161441. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa705-733
Specificity
This SPECC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 705-733 amino acids from the Central region of human SPECC1.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
About SPECC1 / CYTSB

Popular SPECC1 / CYTSB Products

Anti-SPECC1 / CYTSB antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Anti-SPECC1 / CYTSB antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Immunohistochemistry - Paraffin Image
Species: Mouse, Human
Applications: IHC, Western blot
Anti-SPECC1 / CYTSB antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot

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Images

Immunohistochemistry

SPECC1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
SPECC1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Immunofluorescence

Confocal immunofluorescence of SPECC1 Antibody with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of SPECC1 Antibody with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Western blot

SPECC1 Antibody western blot of HL-60 cell line lysates (35 ug/lane). The SPECC1 antibody detected the SPECC1 protein (arrow).
SPECC1 Antibody western blot of HL-60 cell line lysates (35 ug/lane). The SPECC1 antibody detected the SPECC1 protein (arrow).

Western blot

SPECC1 Antibody western blot of mouse NIH-3T3 cell line lysates (35 ug/lane). The SPECC1 antibody detected the SPECC1 protein (arrow).
SPECC1 Antibody western blot of mouse NIH-3T3 cell line lysates (35 ug/lane). The SPECC1 antibody detected the SPECC1 protein (arrow).

Flow Cytometry

SPECC1 Antibody flow cytometry of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
SPECC1 Antibody flow cytometry of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

SPECC1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
SPECC1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Immunofluorescence

Confocal immunofluorescence of SPECC1 Antibody with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of SPECC1 Antibody with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Western blot

SPECC1 Antibody western blot of HL-60 cell line lysates (35 ug/lane). The SPECC1 antibody detected the SPECC1 protein (arrow).
SPECC1 Antibody western blot of HL-60 cell line lysates (35 ug/lane). The SPECC1 antibody detected the SPECC1 protein (arrow).

Western blot

SPECC1 Antibody western blot of mouse NIH-3T3 cell line lysates (35 ug/lane). The SPECC1 antibody detected the SPECC1 protein (arrow).
SPECC1 Antibody western blot of mouse NIH-3T3 cell line lysates (35 ug/lane). The SPECC1 antibody detected the SPECC1 protein (arrow).

Flow Cytometry

SPECC1 Antibody flow cytometry of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
SPECC1 Antibody flow cytometry of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

SPECC1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
SPECC1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Immunofluorescence

Confocal immunofluorescence of SPECC1 Antibody with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of SPECC1 Antibody with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Western blot

SPECC1 Antibody western blot of HL-60 cell line lysates (35 ug/lane). The SPECC1 antibody detected the SPECC1 protein (arrow).
SPECC1 Antibody western blot of HL-60 cell line lysates (35 ug/lane). The SPECC1 antibody detected the SPECC1 protein (arrow).

Western blot

SPECC1 Antibody western blot of mouse NIH-3T3 cell line lysates (35 ug/lane). The SPECC1 antibody detected the SPECC1 protein (arrow).
SPECC1 Antibody western blot of mouse NIH-3T3 cell line lysates (35 ug/lane). The SPECC1 antibody detected the SPECC1 protein (arrow).

Flow Cytometry

SPECC1 Antibody flow cytometry of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
SPECC1 Antibody flow cytometry of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

SPECC1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
SPECC1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Immunofluorescence

Confocal immunofluorescence of SPECC1 Antibody with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of SPECC1 Antibody with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Western blot

SPECC1 Antibody western blot of HL-60 cell line lysates (35 ug/lane). The SPECC1 antibody detected the SPECC1 protein (arrow).
SPECC1 Antibody western blot of HL-60 cell line lysates (35 ug/lane). The SPECC1 antibody detected the SPECC1 protein (arrow).

Western blot

SPECC1 Antibody western blot of mouse NIH-3T3 cell line lysates (35 ug/lane). The SPECC1 antibody detected the SPECC1 protein (arrow).
SPECC1 Antibody western blot of mouse NIH-3T3 cell line lysates (35 ug/lane). The SPECC1 antibody detected the SPECC1 protein (arrow).

Flow Cytometry

SPECC1 Antibody flow cytometry of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
SPECC1 Antibody flow cytometry of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: United States
Date Requested: 1/17/2019
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