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Catalog Number Size Price
LS-C773060-100 100 µl (0.5 mg/ml) $531 
SNCA / Alpha-Synuclein Antibody - Immunohistochemistry analysis using Rabbit Anti-Alpha Synuclein pSer129 Polyclonal Antibody. Tissue: Brain. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-Alpha Synuclein pSer129 Polyclonal Antibody  at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.
SNCA / Alpha-Synuclein Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Alpha Synuclein pSer129 Polyclonal Antibody. Tissue: Primary hippocampal neurons treated with active Alpha Synuclein Protein Aggregate (SPR-322) at 4 µg/ml to induce fibrils. Species: Rat. Fixation: 4% paraformaldehyde. Primary Antibody: Rabbit Anti-Alpha Synuclein pSer129 Polyclonal Antibody  at 1:500 for 24 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit Alexa Fluor 488 at 1:700 for 1 hour at RT. Counterstain: Guinea Pig Anti-NeuN (red) neuronal marker (Donkey Anti-Guinea Pig Alexa Fluor 647 1:700); Hoechst (blue) nuclear stain at 1:6000, 1:3000 for 60 min at RT, 5 min at RT. Magnification: 20X.
SNCA / Alpha-Synuclein Antibody - Western blot analysis of Human, Mouse brain lysate showing detection of ~16 kDa Alpha Synuclein pSer129 protein using Rabbit Anti-Alpha Synuclein pSer129 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Human brain lysate. Lane 3: Mouse brain lysate. Lane 4: Alpha Synuclein Monomer (0.5 µg). Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Alpha Synuclein pSer129 Polyclonal Antibody  at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~16 kDa. Other Band(s): 100, 75, 45, 30,16 kDa.
SNCA / Alpha-Synuclein Antibody - Immunohistochemistry analysis using Rabbit Anti-Alpha Synuclein pSer129 Polyclonal Antibody. Tissue: Brain. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-Alpha Synuclein pSer129 Polyclonal Antibody  at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.
SNCA / Alpha-Synuclein Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Alpha Synuclein pSer129 Polyclonal Antibody. Tissue: Primary hippocampal neurons treated with active Alpha Synuclein Protein Aggregate (SPR-322) at 4 µg/ml to induce fibrils. Species: Rat. Fixation: 4% paraformaldehyde. Primary Antibody: Rabbit Anti-Alpha Synuclein pSer129 Polyclonal Antibody  at 1:500 for 24 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit Alexa Fluor 488 at 1:700 for 1 hour at RT. Counterstain: Guinea Pig Anti-NeuN (red) neuronal marker (Donkey Anti-Guinea Pig Alexa Fluor 647 1:700); Hoechst (blue) nuclear stain at 1:6000, 1:3000 for 60 min at RT, 5 min at RT. Magnification: 20X.
SNCA / Alpha-Synuclein Antibody - Western blot analysis of Human, Mouse brain lysate showing detection of ~16 kDa Alpha Synuclein pSer129 protein using Rabbit Anti-Alpha Synuclein pSer129 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Human brain lysate. Lane 3: Mouse brain lysate. Lane 4: Alpha Synuclein Monomer (0.5 µg). Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Alpha Synuclein pSer129 Polyclonal Antibody  at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~16 kDa. Other Band(s): 100, 75, 45, 30,16 kDa.
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Polyclonal Rabbit anti‑Human SNCA / Alpha‑Synuclein Antibody (Biotin, phospho‑Ser129, IHC, IF, WB) LS‑C773060

Polyclonal Rabbit anti‑Human SNCA / Alpha‑Synuclein Antibody (Biotin, phospho‑Ser129, IHC, IF, WB) LS‑C773060

Antibody:
SNCA / Alpha-Synuclein Rabbit anti-Human Polyclonal (pSer129) (Biotin) Antibody
Application:
IHC, IF, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Biotin, Unmodified
Other formats:
Price
Catalog Number
$531
LS-C773060-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
SNCA / Alpha-Synuclein Rabbit anti-Human Polyclonal (pSer129) (Biotin) Antibody
Application:
IHC, IF, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Biotin, Unmodified
Other formats:

Specifications

Description
Alpha-Synuclein antibody LS-C773060 is a biotin-conjugated rabbit polyclonal antibody to Alpha-Synuclein (SNCA) (pSer129) from human. It is reactive with human, mouse and rat. Validated for ELISA, IF, IHC and WB.
Target
Human SNCA / Alpha-Synuclein
Synonyms
SNCA | Alpha-synuclein | PARK4 | PD1 | Synuclein alpha-140 | NACP | PARK1 | aSynuclein | a-Synuclein
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Biotin. Also available conjugated with APC, Atto 390, Atto 488, Atto 594, FITC, PerCP, RPE.
Purification
Peptide Affinity Purified
Modifications
Unmodified
Immunogen
Synthetic peptide of Human Alpha Synuclein pSer129 (40-140 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).
Epitope
pSer129
Specificity
Binds to phosphorylated serine 129 on alpha synuclein. Does not detect unphosphorylated serine 129 alpha synuclein. Detects bands at 100, 75, 45, 15 kDa. Bands above 15 kDa are oligomers.
Applications
  • IHC
  • Immunofluorescence (1:500)
  • Western blot (1:1000)
  • ELISA
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SNCA / Alpha-Synuclein
P37840 NM_000345 NP_000336.1

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Requested From: United States
Date Requested: 5/3/2024