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Catalog Number Size Price
LS-C815711-100 100 µl $412 
SMAD5 Antibody - Immunofluorescence staining of SMAD5 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human SMAD5 polyclonal antibody (dilution ratio 1:5000) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.
SMAD5 Antibody - Anti-SMAD5 rabbit polyclonal antibody at 1:500 dilution. Lane A: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 52 kDa. Observed band size: 50 kDa.
SMAD5 Antibody - Immunofluorescence staining of SMAD5 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human SMAD5 polyclonal antibody (dilution ratio 1:5000) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.
SMAD5 Antibody - Anti-SMAD5 rabbit polyclonal antibody at 1:500 dilution. Lane A: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 52 kDa. Observed band size: 50 kDa.
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Polyclonal Rabbit anti‑Human SMAD5 Antibody (Preservative Free, IHC, IF, WB) LS‑C815711

Polyclonal Rabbit anti‑Human SMAD5 Antibody (Preservative Free, IHC, IF, WB) LS‑C815711

Antibody:
SMAD5 Rabbit anti-Human Polyclonal (Preservative Free) Antibody
Application:
IHC-P, ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Preservative Free
Price
Catalog Number
$412
LS-C815711-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
SMAD5 Rabbit anti-Human Polyclonal (Preservative Free) Antibody
Application:
IHC-P, ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Preservative Free

Specifications

Description
SMAD5 antibody LS-C815711 is an unconjugated rabbit polyclonal antibody to human SMAD5. Validated for ICC, IF, IHC and WB.
Target
Human SMAD5
Synonyms
SMAD5 | JV5-1 | MADH5 | SMAD 5 | MAD homolog 5 | Mothers against DPP homolog 5 | SMA- and MAD-related protein 5 | SMAD family member 5 | DWFC | HSmad5
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Preservative Free
Immunogen
A synthetic peptide corresponding to the N-Terminus of Human Smad5
Specificity
Human SMAD5
Applications
  • IHC - Paraffin (1:2500 - 1:10000)
  • ICC (1:1500 - 1:50000)
  • Immunofluorescence (1:1500 - 1:50000)
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.2 µm filtered solution in PBS
Storage
Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SMAD5
Q99717 NM_005903 NP_005894.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/27/2024