NHE3 antibody LS-C16766 is an unconjugated rabbit polyclonal antibody to rat NHE3 (SLC9A3). Validated for ELISA and WB.
(tested or 100% immunogen sequence identity)
- Western blot (1 - 10 µg/ml)
- ELISA (1:100000)
SLC9A3 / NHE3 antibody was raised against synthetic peptide consisting of 22aa within the cytoplasmic, C-terminal domain of rat NHE3.
Recognizes rat NHE3. No significant sequence homology exist with other NHE isoforms (NHE1, 2, 4 and 5). Species sequence Homology: Mouse: 90%; human: 76%.
Suitable for use in ELISA and Western Blot. Western Blot: 1-10 ug/ml using ECL; A band of ~90kD has been detected in brush border membrane. ELISA: 1:100000 using 50-100 ng control peptide/well.
100 mM Tris, pH 7.5, 0.05% Sodium Azide, 40% Glycerol, 0.2% BSA
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.