Target
Rat SLC18A1 / VMAT1
Synonyms
SLC18A1 | CGAT | Vesicular amine transporter 1 | VMAT1
Reactivity
Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Immunogen
A 12 AA peptide sequence near the C-Terminus of rat VMAT1 (1). This peptide is predicted to be cytoplasmic. The peptide was synthesized with N-Terminus Cysteine, coupled to KLH.
Specificity
Recognizes rat VMAT1. Does not recognize VMAT2 in transfected cells. The C-terminal rat VMAT1 sequence does not show any appreciable homology with hVMAT1. Antibodies to rVMAT1 are not expected to cross-react with hVMAT1.
Applications
- Western blot (1:1000 - 1:5000)
- ELISA (1:10000 - 1:50000)
Usage
Suitable for use in ELISA, Western Blot. Western Blot: 1:1000-1:5000 for neat serum and 1-10 ug/ml for affinity pure using ECL. A band of 95-110kD has been detected in brush border membrane. ELISA:1:10000-1:50000 for neat serum and 0.5-1 ug/ml for affinity purified. Control peptide can be used to coat ELISA plates at 1 ug/ml.
Presentation
Lyophilized from PBS, 0.05% Sodium Azide
Reconstitution
Reconstitute in 100 ul sterile PBS
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee