VMAT1 antibody LS-C18502 is an unconjugated rabbit polyclonal antibody to rat VMAT1 (SLC18A1). Validated for ELISA and WB.
Rat SLC18A1 / VMAT1
(tested or 100% immunogen sequence identity)
- Western blot (1:1000 - 1:5000)
- ELISA (1:10000 - 1:50000)
SLC18A1 / VMAT1 antibody was raised against a 12 AA peptide sequence near the C-terminus of rat VMAT1 (1). This peptide is predicted to be cytoplasmic. The peptide was synthesized with amino terminal Cysteine, coupled to KLH.
Recognizes rat VMAT1. Does not recognize VMAT2 in transfected cells. The C-terminal rat VMAT1 sequence does not show any appreciable homology with hVMAT1. Antibodies to rVMAT1 are not expected to cross-react with hVMAT1.
Suitable for use in ELISA, Western Blot. Western Blot: 1:1000-1:5000 for neat serum and 1-10 ug/ml for affinity pure using ECL. A band of 95-110kD has been detected in brush border membrane. ELISA:1:10000-1:50000 for neat serum and 0.5-1 ug/ml for affinity purified. Control peptide can be used to coat ELISA plates at 1 ug/ml.
Lyophilized from PBS, 0.05% Sodium Azide
100 µl Reconstitute in 100 ul of Sterile PBS.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.