Detects a major band at ~55kD in postnuclear supernatants of CHO transfected with VMAT1 and not in wild type cells. Some additional bands, both higher and lower MW, were also detected and remain unaffected by the inclusion of protease inhibitors. Species Reactivity: Rat. Not expected to cross-react with human VMAT1.
Western blot: 1-10 ug/ml (Chemiluminescence technique) Immunohistochemistry: 1-20 ug/ml on paraformaldehyde fixed sections. ELISA: 0.5-1.0 ug/ml (1 ug/ml VMAT1 peptide/well).
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
This antibody carries the LSBio 100% Guarantee.