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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SIGLEC6 Antibody LS‑C376620
SIGLEC6 antibody LS-C376620 is an unconjugated rabbit polyclonal antibody to human SIGLEC6. Validated for ELISA, IHC and WB.
Catalog
Size
Price
LS-C376620-50
50 µg
$285
LS-C376620-100
100 µg
$385
Description
SIGLEC6 antibody LS-C376620 is an unconjugated rabbit polyclonal antibody to human SIGLEC6. Validated for ELISA, IHC and WB.
Target
Human SIGLEC6
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with FITC, HRP, Biotin.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:20 - 1:200)
  • Western blot (1:500 - 1:2000)
  • ELISA
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
SIGLEC6 antibody was raised against recombinant human Sialic acid-binding Ig-like lectin 6 protein.
Specificity
Human SIGLEC6
Presentation
PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Storage
Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.
Restrictions
For research use only.
About SIGLEC6
O43699 NM_001245 NP_001236.4

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Immunohistochemistry

Immunohistochemistry of paraffin-embedded human placenta using antibody at dilution of 1:100.
Immunohistochemistry of paraffin-embedded human placenta using antibody at dilution of 1:100.

Western blot

Western blot. All lanes: SIGLEC6 antibody at 6 ug/ml. Lane 1: Human high value serum. Lane 2: A549 whole cell lysate. Lane 3: Caco-2 whole cell lysate. Secondary antibody: Goat polyclonal to Rabbit IgG at 1:10000 dilution. Predicted band size: 50 kDa. Observed band size: 50 kDa.
Western blot. All lanes: SIGLEC6 antibody at 6 ug/ml. Lane 1: Human high value serum. Lane 2: A549 whole cell lysate. Lane 3: Caco-2 whole cell lysate. Secondary antibody: Goat polyclonal to Rabbit IgG at 1:10000 dilution. Predicted band size: 50 kDa. Observed band size: 50 kDa.

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human placenta using antibody at dilution of 1:100.
Immunohistochemistry of paraffin-embedded human placenta using antibody at dilution of 1:100.

Western blot

Western blot. All lanes: SIGLEC6 antibody at 6 ug/ml. Lane 1: Human high value serum. Lane 2: A549 whole cell lysate. Lane 3: Caco-2 whole cell lysate. Secondary antibody: Goat polyclonal to Rabbit IgG at 1:10000 dilution. Predicted band size: 50 kDa. Observed band size: 50 kDa.
Western blot. All lanes: SIGLEC6 antibody at 6 ug/ml. Lane 1: Human high value serum. Lane 2: A549 whole cell lysate. Lane 3: Caco-2 whole cell lysate. Secondary antibody: Goat polyclonal to Rabbit IgG at 1:10000 dilution. Predicted band size: 50 kDa. Observed band size: 50 kDa.

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human placenta using antibody at dilution of 1:100.
Immunohistochemistry of paraffin-embedded human placenta using antibody at dilution of 1:100.

Western blot

Western blot. All lanes: SIGLEC6 antibody at 6 ug/ml. Lane 1: Human high value serum. Lane 2: A549 whole cell lysate. Lane 3: Caco-2 whole cell lysate. Secondary antibody: Goat polyclonal to Rabbit IgG at 1:10000 dilution. Predicted band size: 50 kDa. Observed band size: 50 kDa.
Western blot. All lanes: SIGLEC6 antibody at 6 ug/ml. Lane 1: Human high value serum. Lane 2: A549 whole cell lysate. Lane 3: Caco-2 whole cell lysate. Secondary antibody: Goat polyclonal to Rabbit IgG at 1:10000 dilution. Predicted band size: 50 kDa. Observed band size: 50 kDa.

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human placenta using antibody at dilution of 1:100.
Immunohistochemistry of paraffin-embedded human placenta using antibody at dilution of 1:100.

Western blot

Western blot. All lanes: SIGLEC6 antibody at 6 ug/ml. Lane 1: Human high value serum. Lane 2: A549 whole cell lysate. Lane 3: Caco-2 whole cell lysate. Secondary antibody: Goat polyclonal to Rabbit IgG at 1:10000 dilution. Predicted band size: 50 kDa. Observed band size: 50 kDa.
Western blot. All lanes: SIGLEC6 antibody at 6 ug/ml. Lane 1: Human high value serum. Lane 2: A549 whole cell lysate. Lane 3: Caco-2 whole cell lysate. Secondary antibody: Goat polyclonal to Rabbit IgG at 1:10000 dilution. Predicted band size: 50 kDa. Observed band size: 50 kDa.

Requested From: United States
Date Requested: 11/17/2018
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