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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SIGLEC6 Antibody (aa126‑136) LS‑C139616
SIGLEC6 antibody LS-C139616 is an unconjugated goat polyclonal antibody to human SIGLEC6. Validated for Peptide-ELISA and WB.
Catalog
Size
Price
LS-C139616-100
100 µg
$405

Popular SIGLEC6 Products

Antibody
Species: Human
Applications: Western blot, ELISA
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
Western Blot analysis of SIGLEC6 expression in transfected 293T cell line by SIGLEC6 monoclonal antibody (M02), clone 2G6.Lane 1: SIGLEC6 transfected lysate(48.3 KDa).Lane 2: Non-transfected lysate.
Species: Human
Applications: Western blot, Immunoprecipitation, ELISA
Immunohistochemistry of paraffin-embedded human placenta using antibody at dilution of 1:100.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Immunohistochemistry of Human colon cancer using SIGLEC6 Polyclonal Antibody at dilution of 1:70.
Species: Human
Applications: IHC, Western blot, ELISA

Product Description

SIGLEC6 antibody LS-C139616 is an unconjugated goat polyclonal antibody to human SIGLEC6. Validated for Peptide-ELISA and WB.
About SIGLEC6
Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. O43699 NM_001245 NP_001236.4

SIGLEC6 Antibody, CDW327 Antibody, CD33L1 Antibody, CD327 Antibody, CD327 antigen Antibody, CD33L Antibody, OB-BP1 Antibody, OBBP1 Antibody, Obesity-binding protein 1 Antibody, SIGLEC-6 Antibody, CD33 antigen-like 1 Antibody, CD33L2 Antibody


Specifications

Target
Human SIGLEC6
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.5 - 1.5 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Immunogen
SIGLEC6 antibody was raised against synthetic peptide C-KWMKYGYTSSK from an internal region of human SIGLEC6 (NP_001236.4; NP_942142.3; NP_942143.3). Percent identity by BLAST analysis: Human, Gorilla (100%); Gibbon (82%).
Blocking Peptide
LS-E27438 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C139616. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa126-136
Specificity
Human SIGLEC6. This antibody is expected to recognize all reported isoforms (NP_001236.4; NP_942142.3; NP_942143.3).
Usage
Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Experiments gave bands at approx 60kD and 35kD in Human Placenta lysates. These bands correspond to earlier findings with different antibodies from other commercial sources. This protein has a calculated MW of 49.9kD according to NP_001236.4. Recommended concentration: 0.5-1.5 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.

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Images

Western blot

SIGLEC6 antibody (0.5 ug/ml) staining of Human Placenta lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
SIGLEC6 antibody (0.5 ug/ml) staining of Human Placenta lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

SIGLEC6 antibody (0.5 ug/ml) staining of Human Placenta lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
SIGLEC6 antibody (0.5 ug/ml) staining of Human Placenta lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

SIGLEC6 antibody (0.5 ug/ml) staining of Human Placenta lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
SIGLEC6 antibody (0.5 ug/ml) staining of Human Placenta lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

SIGLEC6 antibody (0.5 ug/ml) staining of Human Placenta lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
SIGLEC6 antibody (0.5 ug/ml) staining of Human Placenta lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: United States
Date Requested: 9/23/2018

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