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SIGLEC6 Antibody (aa126-136) LS-C139616

SIGLEC6 antibody (0.5 ug/ml) staining of Human Placenta lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

SIGLEC6 Antibody (aa126-136) LS-C139616

Antibody:
Goat Polyclonal to Human SIGLEC6
Application:
WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$405 
LS-C139616-100
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Human SIGLEC6
Application:
WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
SIGLEC6 antibody LS-C139616 is an unconjugated goat polyclonal antibody to human SIGLEC6. Validated for Peptide-ELISA and WB.

Target
Human SIGLEC6
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Applications
  • Western blot (0.5 - 1.5 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Immunogen
SIGLEC6 antibody was raised against peptide with sequence C-KWMKYGYTSSK, from the internal region of the protein sequence according to NP_001236.4NP_942142.3NP_942143.3.
Blocking Peptide
LS-E27438 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C139616. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa126-136
Specificity
Human SIGLEC6. This antibody is expected to recognize all reported isoforms (NP_001236.4; NP_942142.3; NP_942143.3).
Usage
Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Experiments gave bands at approx 60kD and 35kD in Human Placenta lysates. These bands correspond to earlier findings with different antibodies from other commercial sources. This protein has a calculated MW of 49.9kD according to NP_001236.4. Recommended concentration: 0.5-1.5 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SIGLEC6
O43699 NM_001245 NP_001236.4

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Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Reactivity: Mouse
Range: 15.6-1000 pg/ml
Reactivity: Human
Range: 0.312-20 ng/ml

Requested From: United States
Date Requested: 7/19/2019
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