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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-B8808-0.05 0.05 mg (1 mg/ml) $545 
SIGLEC15 Antibody - Anti-SIGLEC15 / SIGLEC-15 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
SIGLEC15 Antibody - Western blot analysis of SIGLEC15 in K562 cell lysate with SIGLEC15 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.
SIGLEC15 Antibody - Anti-SIGLEC15 / SIGLEC-15 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
SIGLEC15 Antibody - Western blot analysis of SIGLEC15 in K562 cell lysate with SIGLEC15 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.
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IHCPlus™ SIGLEC15 Antibody Polyclonal ELISA,IHC,WB LS‑B8808

IHCPlus™ SIGLEC15 Antibody Polyclonal ELISA,IHC,WB LS‑B8808

Note: This antibody replaces LS-C153758
Antibody:
Rabbit Polyclonal to Human SIGLEC15
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$545 
LS-B8808-0.05
0.05 mg
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human SIGLEC15
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
SIGLEC15 antibody LS-B8808 is an unconjugated rabbit polyclonal antibody to SIGLEC15 from human, mouse and rat. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human SIGLEC15
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide Column Affinity Chromatography
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1 - 2 µg/ml)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
A 15 amino acid synthetic peptide near the N-Terminus of human SIGLEC15. The immunogen is located within amino acids 50 - 100 of SIGLEC15.
Blocking Peptide
LS-E31246 - Liquid - 0.05 mg - $145.00
Immunizing peptide used to generate LS-B8808. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Specificity
SIGLEC15 antibody is predicted to not cross-react with other SIGLEC family members.
Usage
SIGLEC15 antibody can be used for detection of SIGLEC15 by Western blot at 1 - 2 ug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ug/mL. Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Western Blot: Predicted: 36 kDa Observed: 42 kDa
Presentation
PBS containing 0.02% Sodium Azide
Storage
Store at 4°C for up to 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SIGLEC15
Q6ZMC9 NP_998767.1

Validation

SIGLEC15 Antibody - Anti-SIGLEC15 / SIGLEC-15 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-SIGLEC15 / SIGLEC-15 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-SIGLEC15 / SIGLEC-15 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

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Requested From: United States
Date Requested: 12/12/2019