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Catalog Number Size Price
LS-C45080-0.1 0.1 mg (0.5 mg/ml) $368 
Antibody

Monoclonal Mouse anti‑Rat SIGLEC1 / CD169 / Sialoadhesin Antibody (clone ED3, IHC, IF) LS‑C45080

Monoclonal Mouse anti‑Rat SIGLEC1 / CD169 / Sialoadhesin Antibody (clone ED3, IHC, IF) LS‑C45080

Note: This antibody replaces LS-C45081
Antibody:
SIGLEC1 / CD169 / Sialoadhesin Mouse anti-Rat Monoclonal (ED3) Antibody
Application:
IHC, IHC-Fr, IF, IP, Flo
Reactivity:
Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$368
LS-C45080-0.1
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
SIGLEC1 / CD169 / Sialoadhesin Mouse anti-Rat Monoclonal (ED3) Antibody
Application:
IHC, IHC-Fr, IF, IP, Flo
Reactivity:
Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Sialoadhesin antibody LS-C45080 is an unconjugated mouse monoclonal antibody to rat Sialoadhesin (SIGLEC1 / CD169). Validated for Flow, IF, IHC and IP.
Target
Rat SIGLEC1 / CD169 / Sialoadhesin
Synonyms
SIGLEC1 | CD169 | CD169 antigen | SIGLEC-1 | Sialoadhesin | SN | DJ1009E24.1
Host
Mouse
Reactivity
Rat (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Clone
ED3
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Rat spleen cell homogenate.
Specificity
Recognizes the rat CD169 cell surface antigen, a 185kD molecule expressed by macrophages, predominately confined to lymphoid organs only. Monocytes and granulocytes are negative. No other cell types are positive. The most conspicuous property of ED3 is it stains marginal zone macrophages and marginal metallophils in the spleen very strongly. Furthermore, macrophages in (auto-immune) diseased tissues express the ED3 antigen. In healthy tissue no expression occurs. CD169 is a receptor for glycoconjugates containing sialic acid.
Applications
  • IHC
  • IHC - Frozen (1:250)
  • Immunofluorescence
  • Immunoprecipitation
  • Flow Cytometry (1:500)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request.
Presentation
PBS, 0.09% Sodium Azide
Storage
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SIGLEC1 / CD169 / Sialoadhesin
Q9BZZ2 NM_023068 NP_075556.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/14/2022