LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us

SHH / Sonic Hedgehog

sonic hedgehog

SHH / Sonic Hedgehog is a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. It is also thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities. Additionally, mutations in a long range enhancer located approximately 1 megabase upstream of this gene disrupt limb patterning and can result in preaxial polydactyly.

Gene Name: sonic hedgehog
Family/Subfamily: Protease , Cysteine C46
Synonyms: SHH, HHG-1, HLP3, Sonic hedgehog, Sonic hedgehog homolog, MCOPCB5, Sonic hedgehog protein, HHG1, HPE3, SMMCI, TPT, TPTPS
Target Sequences: NM_000193 NP_000184.1 Q15465

Filtered By: None
Target
SHH / Sonic Hedgehog (75)
Reactivity
Human (42)
Mouse (36)
Rat (31)
Bat (4)
Chicken (4)
Dog (5)
Gibbon (3)
Guinea pig (4)
Hamster (4)
Horse (4)
Monkey (1)
Pig (4)
Primate (2)
Xenopus (4)
Zebrafish (4)
Application
IHC (48)
IHC-P (11)
WB (72)
Flo (3)
ELISA (10)
ICC (4)
IF (5)
Peptide-ELISA (3)
Host
rabbit (57)
mouse (16)
rat (2)
Isotype
IgG (49)
IgG1 (6)
IgG1,k (8)
IgG2a (3)
IgG2a,k (1)
Clonality
monoclonal mc (19)
polyclonal pc (56)
Clone
10H6 (1)
1B11 (1)
3A2 (1)
5H4 (2)
67.8 (1)
8G3 (2)
Format
APC (5)
APC Conjugated (5)
Biotin Conjugated (7)
Cy3 Conjugated (5)
Cy7 Conjugated (5)
FITC Conjugated (9)
HRP Conjugated (6)
PE Conjugated (5)
Unconjugated (33)
Epitope
aa36-193 (10)
aa28-192 (9)
aa28-193 (9)
aa237-399 (8)
N-Terminus (6)
aa397-431 (2)
Glu53 (1)
aa1-75 (1)
aa199-437 (1)
aa203-218 (1)
aa26-170 (1)
aa368-401 (1)
aa58-91 (1)
Publications
No (75)
Showing 1-25 of 75 results

SHH / Sonic Hedgehog Antibody (clone 8G3) IHC-plus™ LS-B8934
Mouse Monoclonal [clone 8G3] (IgG1) to Human SHH / Sonic Hedgehog
Human
ELISA IHC IHC-P WB
Unconjugated
50µl  $425.00
Anti-Sonic Hedgehog / SHH antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Western blot using SHH monoclonal antibody against SHH(AA: 26-161)-hIgGFc transfected HEK293 cell lysate.
Anti-Sonic Hedgehog / SHH antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Western blot using SHH monoclonal antibody against SHH(AA: 26-161)-hIgGFc transfected HEK293 cell lysate.

SHH / Sonic Hedgehog Antibody (N-Terminus) LS-C40460
Rabbit Polyclonal (IgG) to Human SHH / Sonic Hedgehog
Xenopus, Chicken, Zebrafish, Pig, Horse, Rat, Hamster, Mouse, Dog, Guinea pig, Bat, Human
IHC IHC-P WB
Unconjugated
50µg  $365.00
SHH antibody ARP44235_P050-NP_000184-SHH(sonic hedgehog homolog (Drosophila)) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human intestine.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western analysis of SHH in HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SHH antibody ARP44235_P050-NP_000184-SHH(sonic hedgehog homolog (Drosophila)) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human intestine.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western analysis of SHH in HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

SHH / Sonic Hedgehog Antibody (N-Terminus) LS-C122424
Rabbit Polyclonal (IgG) to Human SHH / Sonic Hedgehog
Mouse, Rat, Human
ICC IHC IHC-P WB
Unconjugated
100µg  $530.00

SHH / Sonic Hedgehog Antibody (aa203-218) LS-C343862
Rabbit Polyclonal to Human SHH / Sonic Hedgehog
Mouse, Rat, Human
IHC IHC-P WB
Unconjugated
100µg 200µl 0.5mg/ml  $355.00
Sonic Hedgehog antibody IHC-paraffin: Rat Brain Tissue.
Sonic Hedgehog antibody IHC-paraffin: Human Mammary Tissue.
Sonic Hedgehog antibody IHC-paraffin: Rat Brain Tissue.
Sonic Hedgehog antibody IHC-paraffin: Human Mammary Tissue.

SHH / Sonic Hedgehog Antibody (clone 3A2) LS-C114335
Mouse Monoclonal [clone 3A2] (IgG2a) to Human SHH / Sonic Hedgehog
Human
IF WB
Unconjugated
100µl 0.12mg/ml  $335.00
Anti-SHH mouse monoclonal antibody  immunofluorescent staining  (Red) of COS7 cells transiently transfected by pCMV6-ENTRY SHH.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SHH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SHH.
Anti-SHH mouse monoclonal antibody  immunofluorescent staining  (Red) of COS7 cells transiently transfected by pCMV6-ENTRY SHH.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SHH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SHH.

SHH / Sonic Hedgehog Antibody (clone 5H4) LS-C169089
Mouse Monoclonal [clone 5H4] (IgG1) to Human SHH / Sonic Hedgehog
Human
ELISA Flo IHC IHC-P WB
Unconjugated
100µg 1mg/ml  $365.00
IHC of paraffin-embedded liver cancer tissues using SHH mouse monoclonal antibody with DAB staining.
Western blot using SHH monoclonal antibody against human SHH recombinant protein. (Expected MW is 41 kDa)
IHC of paraffin-embedded liver cancer tissues using SHH mouse monoclonal antibody with DAB staining.
Western blot using SHH monoclonal antibody against human SHH recombinant protein. (Expected MW is 41 kDa)

SHH / Sonic Hedgehog Antibody (aa26-170, clone 5H4) LS-C359325
Mouse Monoclonal [clone 5H4] (IgG1) to Human SHH / Sonic Hedgehog
Mouse, Human, Primate
ELISA Flo IHC IHC-P WB
Unconjugated
100µl  $335.00
Immunohistochemistry-Paraffin: Sonic Hedgehog Antibody (5H4) - Immunohistochemical analysis of paraffin-embedded liver cancer tissues using Sonic Hedgehog mouse mAb with DAB staining.
Western Blot: Sonic Hedgehog Antibody (5H4) - Western blot analysis using Sonic Hedgehog mouse mAb against LNCaP (1), HepG2 (2), PANC-1 (3),HeLa (4), SK-N-SH (5), F9 (6), NIH3T3 (7), and COS7 (8) cell lysate.
Immunohistochemistry-Paraffin: Sonic Hedgehog Antibody (5H4) - Immunohistochemical analysis of paraffin-embedded liver cancer tissues using Sonic Hedgehog mouse mAb with DAB staining.
Western Blot: Sonic Hedgehog Antibody (5H4) - Western blot analysis using Sonic Hedgehog mouse mAb against LNCaP (1), HepG2 (2), PANC-1 (3),HeLa (4), SK-N-SH (5), F9 (6), NIH3T3 (7), and COS7 (8) cell lysate.

SHH / Sonic Hedgehog Antibody (aa1-75) LS-C359327
Rabbit Polyclonal to Human SHH / Sonic Hedgehog
Mouse, Rat, Primate, Human
ICC IF IHC IHC-P WB
Unconjugated
100µl 1mg/ml  $335.00
Immunohistochemistry: Sonic Hedgehog Antibody - IHC analysis of Sonic Hedgehog in mouse kidney.
Immunocytochemistry/Immunofluorescence: Sonic Hedgehog Antibody - Sonic Hedgehog antibody was tested in SH-SY5Y cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry: Sonic Hedgehog Antibody - IHC analysis of Sonic Hedgehog in mouse kidney.
Immunocytochemistry/Immunofluorescence: Sonic Hedgehog Antibody - Sonic Hedgehog antibody was tested in SH-SY5Y cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

SHH / Sonic Hedgehog Antibody (Glu53) LS-C75801
Rabbit Monoclonal (IgG) to Human SHH / Sonic Hedgehog
Human
WB
Unconjugated
100µl  $530.00

SHH / Sonic Hedgehog Antibody LS-C393398
Rabbit Polyclonal to Human SHH / Sonic Hedgehog
Mouse, Rat, Human
IF IHC IHC-P WB
Unconjugated
100µl  $305.00

SHH / Sonic Hedgehog Antibody (clone 10H6) LS-C114336
Mouse Monoclonal [clone 10H6] (IgG1) to Human SHH / Sonic Hedgehog
Dog, Rat, Human, Monkey
IF WB
Unconjugated
100µl  $345.00
Anti-SHH mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SHH.
Anti-SHH mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SHH.
Anti-SHH mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SHH.
Anti-SHH mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SHH.

SHH / Sonic Hedgehog Antibody (N-Terminus, clone 67.8) LS-C354945
Mouse Monoclonal [clone 67.8] (IgG1) to Mouse SHH / Sonic Hedgehog
Rat, Human
IF WB
Unconjugated
100µg  $395.00

SHH / Sonic Hedgehog Antibody LS-C751799
Rabbit Polyclonal (IgG) to Human SHH / Sonic Hedgehog
Human
ELISA IHC WB
Unconjugated
100µg  $355.00

SHH / Sonic Hedgehog Antibody (aa397-431) LS-C356383
Rabbit Polyclonal to Mouse SHH / Sonic Hedgehog
Mouse
Flo Peptide-ELISA WB
Unconjugated
400µl  $305.00
Western blot of lysate from mouse liver tissue lysate, using Shh antibody diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20 ug.
Flow cytometric of HeLa cells using (Mouse) Shh Antibody (green) compared to an isotype control of rabbit IgG (blue) diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Western blot of lysate from mouse liver tissue lysate, using Shh antibody diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20 ug.
Flow cytometric of HeLa cells using (Mouse) Shh Antibody (green) compared to an isotype control of rabbit IgG (blue) diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.

SHH / Sonic Hedgehog Antibody (aa28-193) LS-C294433
Rabbit Polyclonal (IgG) to Mouse SHH / Sonic Hedgehog
Mouse
IHC WB
Unconjugated
20µl 0.5mg/ml  $205.00; 100µl 0.5mg/ml  $255.00; 200µl 0.5mg/ml  $295.00; 1ml 0.5mg/ml  $515.00; 10ml 0.5mg/ml  $2,040.00

SHH / Sonic Hedgehog Antibody (aa28-193, Biotin) LS-C300789
Rabbit Polyclonal (IgG) to Mouse SHH / Sonic Hedgehog
Mouse
IHC WB
Biotin Conjugated
100µl 0.5mg/ml  $425.00

SHH / Sonic Hedgehog Antibody (aa28-193, APC) LS-C740035
Rabbit Polyclonal (IgG) to Mouse SHH / Sonic Hedgehog
Mouse
IHC WB
APC Conjugated
20µl  $225.00; 100µl  $305.00; 200µl  $365.00; 1ml  $770.00

SHH / Sonic Hedgehog Antibody LS-C747600
Rabbit Polyclonal (IgG) to Human SHH / Sonic Hedgehog
Human
IHC WB
Unconjugated
50µl  $235.00
Immunohistochemistry of paraffin-embedded human stomach using SHH antibody (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using SHH antibody (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using SHH antibody (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using SHH antibody (40x lens).

SHH / Sonic Hedgehog Antibody LS-C132078
Rat Monoclonal (IgG2a) to Mouse SHH / Sonic Hedgehog
Mouse
ELISA WB
Unconjugated
500µg  $770.00

SHH / Sonic Hedgehog Antibody (aa199-437) LS-C10144
Rat Monoclonal (IgG2a) to Mouse SHH / Sonic Hedgehog
Mouse
ELISA WB
Unconjugated
100µg  $345.00; 500µg  $770.00

SHH / Sonic Hedgehog Antibody (aa36-193) LS-C314837
Rabbit Polyclonal (IgG) to Human SHH / Sonic Hedgehog
Mouse, Human
IHC WB
Unconjugated
20µl 0.5mg/ml  $205.00; 100µl 0.5mg/ml  $255.00; 200µl 0.5mg/ml  $295.00; 1ml 0.5mg/ml  $495.00; 10ml 0.5mg/ml  $1,980.00
Western blot of recombinant Sonic Hedgehog / SHH.
Western blot of recombinant Sonic Hedgehog / SHH.

SHH / Sonic Hedgehog Antibody LS-C402536
Rabbit Polyclonal (IgG) to Human SHH / Sonic Hedgehog
Mouse, Rat, Human
ELISA IHC
Unconjugated
20µl  $205.00; 60µl  $235.00; 120µl  $295.00; 200µl  $395.00
Immunohistochemistry of Human colon cancer using SHH Polyclonal Antibody at dilution of 1:70.
Immunohistochemistry of Human colon cancer using SHH Polyclonal Antibody at dilution of 1:70.
Immunohistochemistry of Human colon cancer using SHH Polyclonal Antibody at dilution of 1:70.
Immunohistochemistry of Human colon cancer using SHH Polyclonal Antibody at dilution of 1:70.

SHH / Sonic Hedgehog Antibody LS-C405795
Rabbit Polyclonal (IgG) to Human SHH / Sonic Hedgehog
Mouse, Rat, Human
ELISA IHC
Unconjugated
20µl  $205.00; 60µl  $235.00; 120µl  $295.00; 200µl  $395.00
Immunohistochemistry of Human ovarian cancer using SHH Polyclonal Antibody at dilution of 1:40.
Immunohistochemistry of Human ovarian cancer using SHH Polyclonal Antibody at dilution of 1:40.
Immunohistochemistry of Human ovarian cancer using SHH Polyclonal Antibody at dilution of 1:40.
Immunohistochemistry of Human ovarian cancer using SHH Polyclonal Antibody at dilution of 1:40.

SHH / Sonic Hedgehog Antibody (clone 8G3) LS-C171502
Mouse Monoclonal [clone 8G3] (IgG1) to Human SHH / Sonic Hedgehog
Human
ELISA WB
Unconjugated
100µl  $345.00
Western blot of SHH mAb against SHH-hIgGFc transfected HEK293 cell lysate.
Western blot of SHH mAb against SHH-hIgGFc transfected HEK293 cell lysate.

SHH / Sonic Hedgehog Antibody LS-C409276
Rabbit Polyclonal (IgG) to Human SHH / Sonic Hedgehog
Rat, Human
IHC WB
Unconjugated
50µl  $255.00; 100µl  $315.00; 200µl  $425.00
Immunohistochemistry of paraffin-embedded rat kidney tissue.
Immunohistochemistry of paraffin-embedded rat lung tissue.
Immunohistochemistry of paraffin-embedded rat kidney tissue.
Immunohistochemistry of paraffin-embedded rat lung tissue.
Showing 1-25 of 75 results

Selected:

If you do not find the reagent or information you require, please contact customer.support@lsbio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

Get Social With Us!
Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy