SGK3 antibody LS-A106916 is an FITC-conjugated rabbit polyclonal antibody to human SGK3. Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.
(tested or 100% immunogen sequence identity)
SGK3 antibody was raised against synthetic 20 amino acid peptide from N-terminus of human SGK3. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Bat, Horse, Rabbit (95%); Elephant, Panda, Opossum, Turkey, Chicken (90%); Platypus, Lizard, Pufferfish, Zebrafish (85%); Xenopus (80%).
Human SGK3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A7230
was determined to be 5 ug/ml.
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.