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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SGK3 Antibody (aa1‑30) LS‑C99745
SGK3 antibody LS-C99745 is an unconjugated rabbit polyclonal antibody to human SGK3. Validated for IHC and WB.
Catalog
Size
Price
LS-C99745-400
400 µl
$305
Description
SGK3 antibody LS-C99745 is an unconjugated rabbit polyclonal antibody to human SGK3. Validated for IHC and WB.
Target
Human SGK3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E9387 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C99745. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa1-30
Specificity
This SGK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human SGK3.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
About SGK3
Q96BR1 NM_013257 NP_037389.4

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Images

Immunohistochemistry

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with SGK3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with SGK3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunohistochemistry

IHC detection of SGK3 protein on the paraffin sections of the WT (left) and YPC (right) mice at P0 (B and C), P3 (D and E), and P5 (F and G) skin. Positive signals were observed in the cytoplasm of the hair follicle keratinocytes, especially in hair bulb, ORS, IRS, cuticle/cortex and bulge, or sebaceous glands. Some differences between the WT and YPC, for example, the expression in bulb keratinocytes were observed at P3 and P5. Scale bar, 50.
IHC detection of SGK3 protein on the paraffin sections of the WT (left) and YPC (right) mice at P0 (B and C), P3 (D and E), and P5 (F and G) skin. Positive signals were observed in the cytoplasm of the hair follicle keratinocytes, especially in hair bulb, ORS, IRS, cuticle/cortex and bulge, or sebaceous glands. Some differences between the WT and YPC, for example, the expression in bulb keratinocytes were observed at P3 and P5. Scale bar, 50.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot

Western blot of anti-SKG3 antibody in A375 cell lysate. SGK3 (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Western blot of anti-SKG3 antibody in A375 cell lysate. SGK3 (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with SGK3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with SGK3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunohistochemistry

IHC detection of SGK3 protein on the paraffin sections of the WT (left) and YPC (right) mice at P0 (B and C), P3 (D and E), and P5 (F and G) skin. Positive signals were observed in the cytoplasm of the hair follicle keratinocytes, especially in hair bulb, ORS, IRS, cuticle/cortex and bulge, or sebaceous glands. Some differences between the WT and YPC, for example, the expression in bulb keratinocytes were observed at P3 and P5. Scale bar, 50.
IHC detection of SGK3 protein on the paraffin sections of the WT (left) and YPC (right) mice at P0 (B and C), P3 (D and E), and P5 (F and G) skin. Positive signals were observed in the cytoplasm of the hair follicle keratinocytes, especially in hair bulb, ORS, IRS, cuticle/cortex and bulge, or sebaceous glands. Some differences between the WT and YPC, for example, the expression in bulb keratinocytes were observed at P3 and P5. Scale bar, 50.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot

Western blot of anti-SKG3 antibody in A375 cell lysate. SGK3 (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Western blot of anti-SKG3 antibody in A375 cell lysate. SGK3 (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with SGK3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with SGK3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunohistochemistry

IHC detection of SGK3 protein on the paraffin sections of the WT (left) and YPC (right) mice at P0 (B and C), P3 (D and E), and P5 (F and G) skin. Positive signals were observed in the cytoplasm of the hair follicle keratinocytes, especially in hair bulb, ORS, IRS, cuticle/cortex and bulge, or sebaceous glands. Some differences between the WT and YPC, for example, the expression in bulb keratinocytes were observed at P3 and P5. Scale bar, 50.
IHC detection of SGK3 protein on the paraffin sections of the WT (left) and YPC (right) mice at P0 (B and C), P3 (D and E), and P5 (F and G) skin. Positive signals were observed in the cytoplasm of the hair follicle keratinocytes, especially in hair bulb, ORS, IRS, cuticle/cortex and bulge, or sebaceous glands. Some differences between the WT and YPC, for example, the expression in bulb keratinocytes were observed at P3 and P5. Scale bar, 50.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot

Western blot of anti-SKG3 antibody in A375 cell lysate. SGK3 (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Western blot of anti-SKG3 antibody in A375 cell lysate. SGK3 (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with SGK3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with SGK3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunohistochemistry

IHC detection of SGK3 protein on the paraffin sections of the WT (left) and YPC (right) mice at P0 (B and C), P3 (D and E), and P5 (F and G) skin. Positive signals were observed in the cytoplasm of the hair follicle keratinocytes, especially in hair bulb, ORS, IRS, cuticle/cortex and bulge, or sebaceous glands. Some differences between the WT and YPC, for example, the expression in bulb keratinocytes were observed at P3 and P5. Scale bar, 50.
IHC detection of SGK3 protein on the paraffin sections of the WT (left) and YPC (right) mice at P0 (B and C), P3 (D and E), and P5 (F and G) skin. Positive signals were observed in the cytoplasm of the hair follicle keratinocytes, especially in hair bulb, ORS, IRS, cuticle/cortex and bulge, or sebaceous glands. Some differences between the WT and YPC, for example, the expression in bulb keratinocytes were observed at P3 and P5. Scale bar, 50.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot

Western blot of anti-SKG3 antibody in A375 cell lysate. SGK3 (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Western blot of anti-SKG3 antibody in A375 cell lysate. SGK3 (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Requested From: United States
Date Requested: 12/14/2018
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