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Catalog Number Size Price
LS-C745291-25 25 µl (1 mg/ml) $275 
SFRP1 Antibody - Affinity Purified anti-Human SFRP1 antibody was used at a 1:800 dilution for 20 min to detect SFRP in human dermal hypertrophic scar tissue. Tissue was formalin-fixed followed by heat mediated antigen retrieval prior to blocking. HRP Gt-a-Rabbit IgG is suitable for secondary antibody detection.
SFRP1 Antibody - Western blot using the Affinity Purified Anti-SFRP1 antibody shows detection of a band ~37 kDa band (arrowhead) corresponding to SFRP1 in lysates from human cultured airway epithelial cells. Lysates were run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:230 dilution of anti-SFRP1 antibody overnight at 4°C. Signal was detected using standard techniques.
SFRP1 Antibody - ELISA results of purified Rabbit anti-SFRP1 Antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1µg of conjugate. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins)
SFRP1 Antibody - Affinity Purified anti-Human SFRP1 antibody was used at a 1:800 dilution for 20 min to detect SFRP in human dermal hypertrophic scar tissue. Tissue was formalin-fixed followed by heat mediated antigen retrieval prior to blocking. HRP Gt-a-Rabbit IgG is suitable for secondary antibody detection.
SFRP1 Antibody - Western blot using the Affinity Purified Anti-SFRP1 antibody shows detection of a band ~37 kDa band (arrowhead) corresponding to SFRP1 in lysates from human cultured airway epithelial cells. Lysates were run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:230 dilution of anti-SFRP1 antibody overnight at 4°C. Signal was detected using standard techniques.
SFRP1 Antibody - ELISA results of purified Rabbit anti-SFRP1 Antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1µg of conjugate. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins)
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Polyclonal Rabbit anti‑Human SFRP1 Antibody (IHC, IF, WB) LS‑C745291

Polyclonal Rabbit anti‑Human SFRP1 Antibody (IHC, IF, WB) LS‑C745291

Antibody:
SFRP1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C745291-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
SFRP1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
SFRP1 antibody LS-C745291 is an unconjugated rabbit polyclonal antibody to SFRP1 from human. It is reactive with human and mouse. Validated for ELISA, IF, IHC and WB.
Target
Human SFRP1
Synonyms
SFRP1 | FrzA | FRP | SARP-2 | SARP2 | SFRP-1 | FRP-1 | FRP1
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Bovine (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
SFRP1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a 12 aa region of human Sfrp1 protein.
Specificity
A BLAST analysis was used to suggest reactivity with this protein from human based on 100% homology for the immunogen sequence. Expect cross reactivity with SFRP1 from mouse, rat and bovine sources as only a single amino acid residue change is found within the immunogen sequence (91% positive by BLAST). Cross reactivity with SFRP1 homologues from other sources has not been determined.
Applications
  • IHC (1:800)
  • Immunofluorescence (5 µg/ml)
  • Western blot (1:200 - 1:2000)
  • ELISA (1:5000 - 1:25000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SFRP1
Q8N474 NM_003012 NP_003003.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/10/2021