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Catalog Number Size Price
LS-B57-50 50 µg (1 mg/ml) $485 
SFRP1 Antibody - Anti-SFRP1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
SFRP1 Antibody - Affinity Purified anti-Human SFRP1 antibody was used at a 1:800 dilution for 20 min to detect SFRP in human dermal hypertrophic scar tissue. Tissue was formalin-fixed followed by heat mediated antigen retrieval prior to blocking. HRP Gt-a-Rabbit IgG (p/n 611-1302) is suitable for secondary antibody detection.
SFRP1 Antibody - Western Blot - SFRP1 Antibody. Western blot of Affinity Purified anti-SFRP1 antibody shows detection of a band ~37 kD (arrowhead) corresponding to SFRP1 in lysates from human cultured airway epithelial cells. Lysates were run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:230 dilution of anti-SFRP1 antibody overnight at 4C. Signal was detected using standard techniques. Personnel communication Becky Mercer, University of Columbia.
SFRP1 Antibody - Anti-SFRP1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
SFRP1 Antibody - Affinity Purified anti-Human SFRP1 antibody was used at a 1:800 dilution for 20 min to detect SFRP in human dermal hypertrophic scar tissue. Tissue was formalin-fixed followed by heat mediated antigen retrieval prior to blocking. HRP Gt-a-Rabbit IgG (p/n 611-1302) is suitable for secondary antibody detection.
SFRP1 Antibody - Western Blot - SFRP1 Antibody. Western blot of Affinity Purified anti-SFRP1 antibody shows detection of a band ~37 kD (arrowhead) corresponding to SFRP1 in lysates from human cultured airway epithelial cells. Lysates were run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:230 dilution of anti-SFRP1 antibody overnight at 4C. Signal was detected using standard techniques. Personnel communication Becky Mercer, University of Columbia.
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IHC‑plus™ Polyclonal Rabbit anti‑Human SFRP1 Antibody (IHC, IF, WB) LS‑B57

IHC‑plus™ Polyclonal Rabbit anti‑Human SFRP1 Antibody (IHC, IF, WB) LS‑B57

Note: This antibody replaces LS-C18925
Antibody:
SFRP1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-B57-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
SFRP1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
SFRP1 antibody LS-B57 is an unconjugated rabbit polyclonal antibody to SFRP1 from human. It is reactive with human and mouse. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human SFRP1
Synonyms
SFRP1 | FrzA | FRP | SARP-2 | SARP2 | SFRP-1 | FRP-1 | FRP1
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Bovine (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
SFRP1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a 12 aa region of human Sfrp1 protein.
Specificity
A BLAST analysis was used to suggest reactivity with this protein from human based on 100% homology for the immunogen sequence. Expect cross reactivity with SFRP1 from mouse, rat and bovine sources as only a single amino acid residue change is found within the immunogen sequence (91% positive by BLAST). Cross reactivity with SFRP1 homologues from other sources has not been determined.
Applications
  • IHC
  • IHC - Paraffin (20 µg/ml)
  • Immunofluorescence (5 µg/ml)
  • Western blot (1:200 - 1:2000)
  • ELISA (1:5000 - 1:15000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B57 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B57 was determined to be 20 ug/ml.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SFRP1
Q8N474 NM_003012 NP_003003.3

Validation

SFRP1 Antibody - Anti-SFRP1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
Anti-SFRP1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
Anti-SFRP1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.

LSBio Ratings

IHC-plus™ SFRP1 Antibody for IHC, IF/Immunofluorescence, WB/Western, ELISA LS-B57 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/18/2022