Septin 7 antibody LS-C87364 is an unconjugated goat polyclonal antibody to Septin 7 (SEPT7) from human, mouse, rat and other species. Validated for Peptide-ELISA and WB.
Human SEPT7 / Septin 7
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig, Chicken, Xenopus
(tested or 100% immunogen sequence identity)
Zebrafish (at least 90% immunogen sequence identity)
- Western blot (0.3 - 1 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:32000) & (0.3 - 1 µg/ml)
Synthetic peptide C-DNNKNKGQLTKSP from an internal region of human SEPT7 / Septin 7 (NP_001779.3; NP_001011553.2; NP_001229885.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Bovine, Horse, Pig, Chicken, Xenopus, Stickleback (100%); Zebrafish (92%); Salmon (85%).
- Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C87364. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Human SEPT7 / Septin 7. This antibody is expected to recognize all three reported isoforms (NP_001779.3; NP_001011553.2; NP_001229885.1).
Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx 45kD band observed in nuclear lysates of cell line HeLa (calculated MW of 50.7kD according to NP_001779.3 and of 55.6kD according to NP_001011553.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1 ug/ml.
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.