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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SEPT7 / Septin 7

septin 7

SEPT7 / Septin 7 is a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19.

Gene Name: septin 7
Synonyms: SEPT7, CDC10, CDC10 protein homolog, Hcdc10, NBLA02942, Sep7, SEPT7A, Septin 7, Septin-7, CDC3
Target Sequences: NM_001788 NP_001779.3 Q16181

Filtered by: None
Target
SEPT7 / Septin 7 (21)
Reactivity
Human (20)
Mouse (17)
Rat (15)
Bovine (4)
Chicken (2)
Dog (2)
Guinea pig (1)
Hamster (1)
Horse (2)
Monkey (2)
Pig (2)
Rabbit (1)
Xenopus (1)
Yeast (1)
Application
IHC (16)
IHC-P (6)
WB (21)
ELISA (5)
ICC (2)
IF (4)
IP (3)
Peptide-ELISA (3)
Host
rabbit (20)
goat (1)
Isotype
IgG (14)
Clonality
polyclonal pc (21)
Format
Unconjugated (21)
Epitope
320-400 aa, C-terminal (1)
C-Terminus (1)
Met368 (1)
N-Terminus (1)
aa1-278 (1)
aa192-437 (1)
aa308-437 (1)
aa319-368 (1)
aa322-334 (1)
aa350-399 (1)
aa387-437 (1)
aa421-436 (1)
aa75-125 (1)
Publications
No (21)
Showing 1-21 of 21 results

IHC-plus™ SEPT7 / Septin 7 Antibody (aa350-399) LS-B9870
Rabbit Polyclonal (IgG) to Human SEPT7 / Septin 7
Mouse, Rat, Human
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50µl 1mg/ml  $425.00
Anti-SEPT7 / Septin 7 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-SEPT7 / Septin 7 antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

SEPT7 / Septin 7 Antibody (N-Terminus) LS-C144763
Rabbit Polyclonal to Yeast SEPT7 / Septin 7
Yeast
IHC, WB
Unconjugated
100µl  $275.00

SEPT7 / Septin 7 Antibody (C-Terminus) LS-C351955
Rabbit Polyclonal to Human SEPT7 / Septin 7
Mouse, Bovine, Rat, Human
ICC, IF, IHC, IHC-P, IP, WB
Unconjugated
100µl 1mg/ml  $245.00
Immunohistochemical analysis of Septin 7 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of Septin 7 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

SEPT7 / Septin 7 Antibody (320-400 aa, C-terminal) LS-C386019
Rabbit Polyclonal (IgG) to Human SEPT7 / Septin 7
Mouse, Rat, Human
ELISA, ICC, IHC, WB
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $335.00
Western blot of Septin 7 antibody

IHC-plus™ SEPT7 / Septin 7 Antibody (aa319-368) LS-B14700
Rabbit Polyclonal (IgG) to Human SEPT7 / Septin 7
Mouse, Rat, Human
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50µl 1mg/ml  $425.00
Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)

IHC-plus™ SEPT7 / Septin 7 Antibody (aa75-125) LS-B11580
Rabbit Polyclonal (IgG) to Human SEPT7 / Septin 7
Mouse, Human
IHC, IHC-P, IP, WB
Unconjugated
50µl 1mg/ml  $425.00
Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)

SEPT7 / Septin 7 Antibody (aa308-437) LS-C377493
Rabbit Polyclonal (IgG) to Human SEPT7 / Septin 7
Human
ELISA, IHC, IHC-P, WB
Unconjugated
50µl  $285.00; 100µl  $385.00
Immunohistochemistry of paraffin-embedded human brain using antibody at 1:100 dilution.
Immunohistochemistry of paraffin-embedded human brain tissue using SEPT7 Antibody at dilution of 1:100

SEPT7 / Septin 7 Antibody LS-C346129
Rabbit Polyclonal (IgG) to Human SEPT7 / Septin 7
Mouse, Rat, Human
IF, IHC, WB
Unconjugated
50µl  $235.00; 100µl  $315.00; 200µl  $425.00
Immunohistochemistry of paraffin-embedded rat Intestine.
Immunohistochemistry of paraffin-embedded human lung cancer tissue.

SEPT7 / Septin 7 Antibody LS-C772551
Rabbit Polyclonal (IgG) to Human SEPT7 / Septin 7
Mouse, Rat, Human
IF, IHC, WB
Unconjugated
60µl 1mg/ml  $235.00; 120µl 1mg/ml  $295.00; 200µl 1mg/ml  $395.00

SEPT7 / Septin 7 Antibody LS-C805374
Rabbit Polyclonal (IgG) to Human SEPT7 / Septin 7
Mouse, Rat, Human
ELISA, IF, WB
Unconjugated
100µl 1mg/ml  $325.00; 200µl 1mg/ml  $375.00
Immunofluorescence Image
Western blot Image

SEPT7 / Septin 7 Antibody LS-C771962
Rabbit Polyclonal (IgG) to Human SEPT7 / Septin 7
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
20µl 0.5mg/ml  $205.00; 60µl 0.5mg/ml  $235.00; 120µl 0.5mg/ml  $295.00; 200µl 0.5mg/ml  $395.00

SEPT7 / Septin 7 Antibody LS-C770522
Rabbit Polyclonal (IgG) to Human SEPT7 / Septin 7
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
20µl 0.6mg/ml  $205.00; 60µl 0.6mg/ml  $235.00; 120µl 0.6mg/ml  $295.00; 200µl 0.6mg/ml  $395.00

SEPT7 / Septin 7 Antibody (aa387-437) LS-C290854
Rabbit Polyclonal (IgG) to Human SEPT7 / Septin 7
Mouse, Human
IHC, IP, WB
Unconjugated
100µl 1mg/ml  $395.00
Detection of Human and Mouse Septin 7 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-Septin 7 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.

SEPT7 / Septin 7 Antibody (aa192-437) LS-C185413
Rabbit Polyclonal to Human SEPT7 / Septin 7
Bovine, Human
IHC, IHC-P, WB
Unconjugated
100µl 1.02mg/ml  $355.00
IHC of paraffin-embedded GASTRIC CA using 39331 antibody at 1:100 dilution.
Sample (30 ug of whole cell lysate). A: HeLa S3. 7.5% SDS PAGE. Septin 7 / SEPT7 antibody diluted at 1:1000

SEPT7 / Septin 7 Antibody LS-C770523
Rabbit Polyclonal (IgG) to Human SEPT7 / Septin 7
Mouse, Rat, Human
IHC, WB
Unconjugated
60µl 1mg/ml  $235.00; 120µl 1mg/ml  $295.00; 200µl 1mg/ml  $395.00

SEPT7 / Septin 7 Antibody (Met368) LS-C192138
Rabbit Polyclonal to Human SEPT7 / Septin 7
Mouse, Rat, Human
IHC, WB
Unconjugated
100µl  $345.00

SEPT7 / Septin 7 Antibody (aa421-436) LS-C194049
Rabbit Polyclonal (IgG) to Human SEPT7 / Septin 7
Chicken, Bovine, Rabbit, Mouse, Dog, Guinea pig, Rat, Pig, Horse, Human, Monkey
IHC, WB
Unconjugated
100µg 1ml  $860.00

SEPT7 / Septin 7 Antibody (aa322-334) LS-C87364
Goat Polyclonal to Human SEPT7 / Septin 7
Xenopus, Chicken, Bovine, Rat, Hamster, Pig, Horse, Mouse, Dog, Human, Monkey
Peptide-ELISA, WB
Unconjugated
100µg 0.5mg/ml  $405.00
Antibody (0.5 ug/ml) staining of nuclear HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence

SEPT7 / Septin 7 Antibody LS-C356989
Rabbit Polyclonal to Human SEPT7 / Septin 7
Mouse, Rat, Human
WB
Unconjugated
100µl  $325.00
All lanes : Anti-Septin 7 Antibody at 1:1000 dilution Lane 1: Jurkat whole cell lysates Lane 2: MCF-7 whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 51 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

SEPT7 / Septin 7 Antibody LS-C335403
Rabbit Polyclonal (IgG) to Human SEPT7 / Septin 7
Mouse, Rat, Human
WB
Unconjugated
50µl  $235.00; 100µl  $295.00; 200µl  $385.00
Western blot analysis of extracts of various cell lines, using SEPT7 antibody.

SEPT7 / Septin 7 Antibody (aa1-278) LS-C185414
Rabbit Polyclonal to Human SEPT7 / Septin 7
Human
WB
Unconjugated
100µl 0.31mg/ml  $355.00
Sample (30 ug of whole cell lysate). A: MOLT4 . 7.5% SDS PAGE. Septin 7 / SEPT7 antibody diluted at 1:500
Showing 1-21 of 21 results

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If you do not find the reagent or information you require, please contact customer.support@lsbio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).