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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SEPT7 / Septin 7 Antibody (aa1‑278) LS‑C185414
Septin 7 antibody LS-C185414 is an unconjugated rabbit polyclonal antibody to human Septin 7 (SEPT7). Validated for WB.
Catalog
Size
Price
LS-C185414-100
100 µl (0.31 mg/ml)
$335

Popular SEPT7 / Septin 7 Products

Antibody
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Western blot of Septin 7 antibody
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Immunohistochemical analysis of Septin 7 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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Species: Human, Mouse, Rat, Bovine, Dog, Horse, Rabbit, Chicken
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation

Product Description

SEPT7 / Septin 7 Antibody (aa1-278) for WB/Western LS-C185414

Specifications

Target
Human SEPT7 / Septin 7
Synonyms
SEPT7, CDC10, CDC10 protein homolog, Hcdc10, NBLA02942, Sep7, SEPT7A, Septin 7, Septin-7, CDC3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:500 - 1:3000)
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 278 of SEPT7 (SwissProt Q16181). Percent identity by BLAST analysis: Human (100%); Rat (99%); Mouse (98%).
Epitope
aa1-278
Specificity
Human Septin 7 / SEPT7
Presentation
0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Restrictions
For research use only.
About SEPT7 / Septin 7
SEPT7 / Septin 7 is a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. Q16181 NM_001788 NP_001779.3


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Images

Western blot

Sample (30 ug of whole cell lysate). A: MOLT4 . 7.5% SDS PAGE. Septin 7 / SEPT7 antibody diluted at 1:500
Sample (30 ug of whole cell lysate). A: MOLT4 . 7.5% SDS PAGE. Septin 7 / SEPT7 antibody diluted at 1:500

Western blot

Sample (30 ug of whole cell lysate). A: MOLT4 . 7.5% SDS PAGE. Septin 7 / SEPT7 antibody diluted at 1:500
Sample (30 ug of whole cell lysate). A: MOLT4 . 7.5% SDS PAGE. Septin 7 / SEPT7 antibody diluted at 1:500

Western blot

Sample (30 ug of whole cell lysate). A: MOLT4 . 7.5% SDS PAGE. Septin 7 / SEPT7 antibody diluted at 1:500
Sample (30 ug of whole cell lysate). A: MOLT4 . 7.5% SDS PAGE. Septin 7 / SEPT7 antibody diluted at 1:500

Western blot

Sample (30 ug of whole cell lysate). A: MOLT4 . 7.5% SDS PAGE. Septin 7 / SEPT7 antibody diluted at 1:500
Sample (30 ug of whole cell lysate). A: MOLT4 . 7.5% SDS PAGE. Septin 7 / SEPT7 antibody diluted at 1:500

Requested From: United States
Date Requested: 10/17/2018

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