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Catalog Number Size Price
LS-C25941-200 200 µg $702 
Antibody

Polyclonal Rabbit anti‑Rat RPS6KB1 / P70S6K / S6K Antibody (aa26‑43, WB) LS‑C25941

Polyclonal Rabbit anti‑Rat RPS6KB1 / P70S6K / S6K Antibody (aa26‑43, WB) LS‑C25941

Antibody:
RPS6KB1 / P70S6K / S6K Rabbit anti-Rat Polyclonal (aa26-43) Antibody
Application:
WB, IP, Func
Reactivity:
Rat, Human, Mouse
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$702
LS-C25941-200
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
RPS6KB1 / P70S6K / S6K Rabbit anti-Rat Polyclonal (aa26-43) Antibody
Application:
WB, IP, Func
Reactivity:
Rat, Human, Mouse
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
S6K antibody LS-C25941 is an unconjugated rabbit polyclonal antibody to S6K (RPS6KB1 / P70S6K) (aa26-43) from rat. It is reactive with human, mouse and rat. Validated for Func, IP and WB.
Target
Rat RPS6KB1 / P70S6K / S6K
Synonyms
RPS6KB1 | 70 kDa S6 kinase | p70 S6KA | p70-alpha | p70-S6K | p70 S6 kinase alpha | p70s6 kinase | S6K-beta-1 | STK14A | p70 ribosomal S6 kinase | p70 S6 kinase, alpha 1 | p70 S6 kinase, alpha 2 | p70 S6K-alpha | p70-S6K 1 | p70S6K | p70S6K1 | PS6K | Ribosomal protein S6 kinase I | S6K | S6K1 | p70 ribosomal S6 kinase alpha | p70(S6K)-alpha
Host
Rabbit
Reactivity
Rat, Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Synthetic peptide corresponding to residues 26-43 of rat S6 Kinase. The immunizing sequence is identical in mouse and human.
Epitope
aa26-43
Specificity
Recognizes p70 S6 kinase Mr ~64kD. In some cell lysates, an additional protein may be detected at a higher molecular weight. Cross-reacts with rat, human and mouse.
Applications
  • Western blot
  • Immunoprecipitation
  • Functional Assay
Usage
Suitable for use in Western Blot, Immunoprecipitation. Western Blot Analysis: 0.5-2.0 ug/ml detects p70 S6 kinase in RIPA lysates from HEK293, PC-12, and A431 cells. HEK293 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-p70 S6 kinase (2 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4 ug immunoprecipitates p70 S6 kinase from 500 ug of HEK293 RIPA lysate. Immunoprecipitation Kinase Assay: 5 ug immunoprecipitates active p70 S6 kinase from IGF-1 stimulated (15nM) human HEK293 cell lysate containing 1 uM microcystin. The assay was performed using a S6 Kinase Assay Kit, which uses specific substrate peptide as a kinase substrate and contains a separate inhibitor cocktail which blocks the activity of other serine/threonine kinases such as protein kinase A, protein kinase C, and calmodulin dependent kinases.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RPS6KB1 / P70S6K / S6K
P23443 NM_003161 NP_003152.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/18/2022