(tested or 100% immunogen sequence identity)
- IHC (1:200 - 1:1000)
- Western blot (1:500 - 1:2500)
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This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with highly purified R-Phycoerythrin from the seaweed gracilaria.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and R-Phycoerythrin conjugated IgG. This antibody will cross react with B-Phycoerythrin. Reactivity with other phycobiliproteins is unknown.
Suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. R-Phycoerythrin (240 kD) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents. Reaction with low molecular fragments is typically noted by western blot. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
0.05 M Tris chloride, 0.15 M sodium chloride, 1 mM magnesiuim chloride, 0.1 mM zinc chloride, pH 8.0, 50% glycerol, 1% BSA, 0.01% sodium azide, sterile filtered
Store at 4°C. Do not freeze.
For research use only.
This antibody carries the LSBio 100% Guarantee.