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Catalog Number Size Price
LS-C801037-100 100 µl (1 mg/ml) $379 
LS-C801037-200 200 µl (1 mg/ml) $421 
RPA2 / RFA2 / RPA34 Antibody - 1:200 staining human brain tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
RPA2 / RFA2 / RPA34 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
RPA2 / RFA2 / RPA34 Antibody - Western blot analysis of RFA2 phosphorylation expression in Adriamycin treated HeLa whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
RPA2 / RFA2 / RPA34 Antibody - Western blot analysis of Phospho-RFA2 (Thr21) expression in various lysates
RPA2 / RFA2 / RPA34 Antibody - 1:200 staining human brain tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
RPA2 / RFA2 / RPA34 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
RPA2 / RFA2 / RPA34 Antibody - Western blot analysis of RFA2 phosphorylation expression in Adriamycin treated HeLa whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
RPA2 / RFA2 / RPA34 Antibody - Western blot analysis of Phospho-RFA2 (Thr21) expression in various lysates
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Polyclonal Rabbit anti‑Human RPA2 / RFA2 / RPA34 Antibody (phospho‑Thr21, IHC, IF, WB) LS‑C801037

Polyclonal Rabbit anti‑Human RPA2 / RFA2 / RPA34 Antibody (phospho‑Thr21, IHC, IF, WB) LS‑C801037

Antibody:
RPA2 / RFA2 / RPA34 Rabbit anti-Human Polyclonal (pThr21) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C801037-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
RPA2 / RFA2 / RPA34 Rabbit anti-Human Polyclonal (pThr21) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
RPA34 antibody LS-C801037 is an unconjugated rabbit polyclonal antibody to RPA34 (RPA2 / RFA2) (pThr21) from human. It is reactive with human and mouse. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human RPA2 / RFA2 / RPA34
Synonyms
RPA2 | RF-A protein 2 | RP-A p34 | RPA34 | Replication protein A2, 32kDa | RP-A p32 | REPA2 | Replication factor A protein 2 | Replication protein A2 (32kD) | RPA32
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human RFA2 around the phosphorylation site of Threonine 21.
Epitope
pThr21
Specificity
Phospho-RFA2 (Thr21) Antibody detects endogenous levels of RFA2 only when phosphorylated at Threonine 21.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RPA2 / RFA2 / RPA34
P15927 NM_002946 NP_002937.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 2/24/2024