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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C154242-100 100 µg (1.39 mg/ml) $425 
RBL2 / RB2 p130 Antibody - Anti-Spa310 Antibody - Western Blot. Western blot of affinity purified anti-Spa310 antibody shows detection of endogenous pRb2/p130 protein in whole LNCaP cell extracts. The band at ~130 kD, indicated by the arrowhead, corresponds to the expected molecular weight of pRb2/p130. The membrane was blocked overnight with a milk buffer at 4C. Primary antibody was diluted 1:500 and reacted with the membrane overnight at 4C. ECL was used for detection. Personal communication, Ang Sun, Sbarro Institute for Cancer Research and Molecular Medicine, Temple University, Philadelphia, PA.

RBL2 / RB2 p130 Antibody LS‑C154242

RBL2 / RB2 p130 Antibody LS‑C154242

Antibody:
Rabbit Polyclonal to Human RBL2 / RB2 p130
Application:
WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C154242-100
100 µg
1.39 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human RBL2 / RB2 p130
Application:
WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
RB2 p130 antibody LS-C154242 is an unconjugated rabbit polyclonal antibody to human RB2 p130 (RBL2 ). Validated for ELISA, IP and WB.
Target
Human RBL2 / RB2 p130
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Applications
  • Western blot (1:500 - 1:5000)
  • Immunoprecipitation (1:100)
  • ELISA
Immunogen
This affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to the Spa310 sequence of pRb2/p130 protein. A residue of cysteine was added to facilitate coupling.
Specificity
This antiserum is directed against Spa310 and reacts with the Spa3120 domain of pRb2/p130 from human tissues. Based on the sequence we expect this antibody to react as well Spa310 from chimpanzee and orangutan, and with lesser affinity, Spa310 from horse, dog, bovine, rat and opossum.
Usage
This affinity purified antibody is suitable for ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 130 kD in size corresponding to pRb2/p130, and a band approximately 4.2kD in size corresponding to Spa310 peptide (latter not shown), by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.1% sodium azide, sterile filtered
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RBL2 / RB2 p130
Q08999 NM_005611 NP_005602.3

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Requested From: United States
Date Requested: 11/15/2019