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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Rabbit Anti‑Rat IgM Secondary Antibody (HRP) LS‑C70377
Antibody
Rat IgM antibody LS-C70377 is an HRP-conjugated rabbit polyclonal antibody. Validated for ELISA, IHC and WB.
Catalog
Size
Price
LS-C70377-500
500 µg (1.5 mg/ml)
Unavailable

Popular Rat IgM Products

Antibody
Species: Rat
Applications: IHC, Western blot, ELISA
Antibody
Species: Rat
Applications: Immunofluorescence
Antibody
Species: Rat
Applications: IHC, Western blot, ELISA

Product Description

Rat IgM antibody LS-C70377 is an HRP-conjugated rabbit polyclonal antibody. Validated for ELISA, IHC and WB.
About Rat IgM
Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen. The spleen where plasmablasts reside is the major site of specific IgM production. IgM forms polymers where multiple immunoglobulins are covalently linked together with disulfide bonds, mostly as a pentamer but also as a hexamer.

Specifications

Target
Rat IgM
Host
Rabbit
Reactivity
Rat (tested or 100% immunogen sequence identity)
Clonality
IgM Polyclonal
Conjugations
Horseradish Peroxidase
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • Western blot
  • ELISA (1:7500)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Rat IgM antibody was raised against rat IgM mu heavy chain.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Rat IgM and Rat Serum. No reaction was observed against Rat IgA or Rat IgG. ELISA was used to confirm specificity at less than 1% cross reactivity against other rat heavy or light chain isotypes.
Usage
Suitable for use in immunoblot (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry. ELISA: 1:7500 to 1:30000, This product has been assayed against 1.0 ug of Rat IgG in a standard capture using ABTS as a substrate for 30 minutes at room temperature. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, pH 7.2, 10 mg/ml BSA (IgG and Protease free), 0.01% Gentamicin sulfate.
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/22/2018

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