(tested or 100% immunogen sequence identity)
- Western blot
- ELISA (1:7500)
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Rat IgM antibody was raised against rat IgM mu heavy chain.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Rat IgM and Rat Serum. No reaction was observed against Rat IgA or Rat IgG. ELISA was used to confirm specificity at less than 1% cross reactivity against other rat heavy or light chain isotypes.
Suitable for use in immunoblot (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry. ELISA: 1:7500 to 1:30000, This product has been assayed against 1.0 ug of Rat IgG in a standard capture using ABTS as a substrate for 30 minutes at room temperature. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
PBS, pH 7.2, 10 mg/ml BSA (IgG and Protease free), 0.01% Gentamicin sulfate.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
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