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Rabbit Anti-Rat IgG Fab'2 Secondary Antibody (Biotin) LS-C70065

Ordering

Wt. Vol. Conc. Price
2000 µg 1000 µl - $475
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
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Rabbit Polyclonal (IgG) to Rat IgG Fab'2
Rat
IHC, Immunofluorescence, Western blot, ELISA
Biotin Conjugated

Details

Rat IgG Fab'2
Rabbit
Rat (tested or 100% immunogen sequence identity)
IgG Polyclonal
Biotin
Affinity purified
Unmodified

Applications

  • IHC
  • Immunofluorescence
  • Western blot
  • ELISA (1:6000)

Specificity and Use

Rat IgG Fab'2 antibody was raised against rat IgG F (ab')2 fragment.
Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-rabbit serum, rat IgG and rat serum and rat IgG F (ab')2. No reaction was observed against rat IgG F(c).
Western Blot, ELISA, Immunohistochemistry, Immunomicroscopy and antibody based assays using streptavidin or avidin conjugates. Assayed against 1 ug of Rat IgG in a standard capture ELISA using peroxidase conjugated streptavidin. A working dilution of 1:6000 to 1:25000 of the reconstitution concentration is suggested. Storage Conditions: Store vial at 4? C prior to restoration. Restore with 1 ml of deionized water (or equivalent). For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at-20? C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4? C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one year from date of restoration. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

Lyophilized from PBS, pH 7.2, 10 mg/ml BSA, 0.01% Thimerosal.
1,000 µl deionized water
For research use only.

About Rat IgG Fab'2

The fragment antigen-binding (Fab fragment) is a region on an antibody that binds to antigens. It is composed of one constant and one variable domain of each of the heavy and the light chain. These domains shape the paratope — the antigen-binding site — at the amino terminal end of the monomer. The two variable domains bind the epitope on their specific antigens. In an experimental setting, Fc and Fab fragments can be generated in the laboratory.

Requested From: United States
Date Requested: 12/9/2016

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