Reactivity
Rat
(tested or 100% immunogen sequence identity)
Conjugations
Phycoerythrin
Purification
Immunoaffinity purified
Immunogen
Rat IgG F (ab')2 fragment.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Rat IgG, Rat IgG F (ab')2 and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(c) or Bovine, Equine and Human Serum Proteins.
Applications
- Immunofluorescence
- Flow Cytometry (1:100)
- ELISA
Usage
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Applications: Suitable for use in Flow Cytometry, ELISA and Immunofluorescence. Flow Cytometry: 1:100 to 1:500 (~1 ug will stain 1x1E6 cells). The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, pH 7.6, 15 mg/ml BSA (IgG, protease free), 0.05% sodium azide.
Storage
Store at 4°C. Do not freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee