(tested or 100% immunogen sequence identity)
- IHC (1:500 - 1:2500)
- Western blot (1:2000 - 1:10000)
- ELISA (1:75000)
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Mouse IgG2a antibody was raised against mouse IgG2a (g2a chain).
Recognizes Mouse IgG2a. Assay by IEP resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum. Specificity was confirmed by ELISA at less than 1% cross-reactivity against other mouse or human heavy or light chain isotypes.
Suitable for use in Western Blot (Western or Dot Blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry. ELISA: 1:75000. Western Blot: 1:2000-1:10000. Immunohistochemistry: 1:500-1:2500. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Lyophilized from PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% gentamicin sulfate
1,000 µl Sterile ddH2O
Lyophilized powder may be stored at -20°C. Reconstituted product is stable for 1 year at -20°C. Labeled with horseradish peroxidase (HRP). Protect from light.
For research use only.
This antibody carries the LSBio 100% Guarantee.